cDNA Clone DCAF19 (BRWD1)

• Synonyms: C21orf107; DCAF19; N143; WDR9; WRD9; BRWD1
• Plasmid: pcDNA5 FRT/TO GFP NLS DCAF19 tv3 (isoform C)
• Parent Plasmid: pcDNA5 FRT TO GFP
• Expressed: GFP-NLS-DCAF19 tv3 (isoform C)
• Genbank: NM_001007246.2 with residue change Q83E (seen in other published sequences)
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGGCGGAGCCGTCGTCCGCCCGACGCCCGGTGCCTCTCATCGAGTCGGAGCTGTACTTCCTTATCGCCCGGTACCTATCGGCGGGCCCGTGTCGGAGAGCGGCCCAGGTGCTGGTGCAGGAGCTGGAGCAGTACCAGTTGTTGCCGAAGAGATTGGACTGGGAGGGCAACGAGCACAACAGGAGCTACGAGGAGTTGGTCTTGTCCAATAAGCATGTGGCTCCTGATCATCTTTTGCAAATCTGCGAGCGCATCGGTCCTATGTTGGATAAAGAAATTCCACCCAGTATTTCAAGAGTCACTTCTTTACTTGGTGCAGGAAGGCAGTCTTTGCTACGTACAGCAAAAGGTACCTTAATTTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLPPKKKRKVGSMAEPSSARRPVPLIESELYFLIARYLSAGPCRRAAQVLVQELEQYQLL
PKRLDWEGNEHNRSYEELVLSNKHVAPDHLLQICERIGPMLDKEIPPSISRVTSLLGAGRQSLLRTAKGTLI*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU63650
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Isoform c is a short isoform of this protein. Attempts to amplify longer isoforms from RNA sources were unsuccessful.
• Low Temp: No