cDNA Clone TRPC4AP

• Synonyms: TRUSS; TRRP4AP; PPP1R158; C20orf188
• Plasmid: pcDNA5 FRT/TO GFP NLS TRPC4AP
• Parent Plasmid: pcDNA5 FRT/TO GFP NLS
• Expressed: GFP NLS TRPC4AP
• Genbank: NM_015638.2
• Tag: GFP
• Tag: NLS
• Species: Human

Sequence of Insert

GGATCCATGGCGGCGGCGCCGGTAGCGGCTGGGTCTGGAGCCGGCCGAGGGAGACGGTCGGCAGCCACAGTGGCGGCTTGGGGCGGATGGGGCGGCCGGCCGCGGCCTGGTAACATTCTGCTGCAGCTGCGGCAGGGCCAGCTGACCGGCCGGGGCCTGGTCCGGGCGGTGCAGTTCACTGAGACTTTTTTGACGGAGAGGGACAAACAATCCAAGTGGAGTGGAATTCCTCAGCTGCTCCTCAAGCTGCACACCACCAGCCACCTCCACAGTGACTTTGTTGAGTGTCAAAACATCCTCAAGGAAATTTCTCCTCTTCTCTCCATGGAGGCTATGGCATTTGTTACTGAAGAGAGGAAACTTACCCAAGAAACCACTTATCCAAATACTTATATTTTTGACTTGTTTGGAGGTGTTGATCTTCTTGTAGAAATTCTTATGAGGCCTACGATCTCTATCCGGGGACAGAAACTGAAAATAAGTGATGAAATGTCCAAGGACTGCTTGAGTATCCTGTATAATACCTGTGTCTGTACAGAGGGAGTTACAAAGCGTTTGGCAGAAAAGAATGACTTTGTGATCTTCCTGTTTACATTGATGACAAGTAAGAAGACATTCTTACAAACAGCAACCCTCATTGAAGATATTTTGGGTGTTAAAAAGGAAATGATCCGACTAGATGAAGTCCCCAATCTGAGTTCCTTAGTATCCAATTTCGATCAGCAGCAGCTCGCTAATTTCTGCCGGATTCTGGCTGTCACCATTTCAGAGATGGATACAGGGAATGATGACAAGCACACGCTTCTTGCCAAAAATGCTCAACAGAAGAAGAGCTTGAGTTTGGGGCCTTCTGCAGCTGAAATCAATCAAGCGGCCCTTCTCAGCATTCCTGGCTTTGTTGAGCGGCTTTGCAAACTGGCGACTCGAAAGGTGTCAGAGTCAACGGGCACAGCCAGCTTCCTTCAGGAGTTGGAAGAGTGGTACACATGGCTAGACAATGCTTTGGTGCTAGATGCCCTGATGCGAGTGGCCAATGAGGAGTCAGAGCACAATCAAGCCTCCATTGTGTTCCCTCCTCCAGGGGCTTCTGAGGAGAATGGCCTGCCTCACACGTCAGCCAGAACCCAGCTGCCCCAGTCAATGAAGATTATGCATGAGATCATGTACAAACTGGAAGTGCTCTATGTCCTCTGCGTGCTGCTGATGGGGCGTCAGCGAAACCAGGTTCACAGAATGATTGCAGAGTTCAAGCTGATCCCTGGACTTAATAATTTGTTTGACAAACTGATTTGGAGGAAGCATTCAGCATCTGCCCTTGTCCTCCATGGTCACAACCAGAACTGTGACTGTAGCCCGGACATCACCTTGAAGATACAGTTTTTGAGGCTTCTTCAGAGCTTCAGTGACCACCACGAGAACAAGTACTTGTTACTCAACAACCAGGAGCTGAATGAACTCAGTGCCATCTCTCTCAAGGCCAACATCCCTGAGGTGGAAGCTGTCCTCAACACCGACAGGAGTTTGGTGTGTGATGGGAAGAGGGGCTTATTAACTCGTCTGCTGCAGGTCATGAAGAAGGAGCCAGCAGAGTCGTCTTTCAGGTTTTGGCAAGCTCGGGCTGTGGAGAGTTTCCTCCGAGGGACCACCTCCTATGCAGACCAGATGTTCCTGCTGAAGCGAGGCCTCTTGGAGCACATCCTTTACTGCATTGTGGACAGCGAGTGTAAGTCAAGGGATGTGCTCCAGAGTTACTTTGACCTCCTGGGGGAGCTGATGAAGTTCAACGTTGATGCATTCAAGAGATTCAATAAATATATCAACACCGATGCAAAGTTCCAGGTATTCCTGAAGCAGATCAACAGCTCCCTGGTGGACTCCAACATGCTGGTGCGCTGTGTCACTCTGTCCCTGGACCGATTTGAAAACCAGGTGGATATGAAAGTTGCCGAGGTACTGTCTGAATGCCGCCTGCTCGCCTACATATCCCAGGTGCCCACGCAGATGTCCTTCCTCTTCCGCCTCATCAACATCATCCACGTGCAGACGCTGACCCAGGAGAACGTCAGCTGCCTCAACACCAGCCTGGTGATCCTGATGCTGGCCCGACGGAAAGAGCGGCTGCCCCTGTACCTGCGGCTGCTGCAGCGGATGGAGCACAGCAAGAAGTACCCCGGCTTCCTGCTCAACAACTTCCACAACCTGCTGCGCTTCTGGCAGCAGCACTACCTGCACAAGGACAAGGACAGCACCTGCCTAGAGAACAGCTCCTGCATCAGCTTCTCATACTGGAAGGAGACAGTGTCCATCCTGTTGAACCCGGACCGGCAGTCACCCTCTGCTCTCGTTAGCTACATTGAGGAGCCCTACATGGACATAGACAGGGACTTCACTGAGGAGTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLPPKKKRKVGSMAAAPVAAGSGAGRGRRSAATVAAWGGWGGRPRPGNILLQLRQGQLTG
RGLVRAVQFTETFLTERDKQSKWSGIPQLLLKLHTTSHLHSDFVECQNILKEISPLLSMEAMAFVTEERKLTQETTYPNTYIFDLFGGVDLLVEILMRPT
ISIRGQKLKISDEMSKDCLSILYNTCVCTEGVTKRLAEKNDFVIFLFTLMTSKKTFLQTATLIEDILGVKKEMIRLDEVPNLSSLVSNFDQQQLANFCRI
LAVTISEMDTGNDDKHTLLAKNAQQKKSLSLGPSAAEINQAALLSIPGFVERLCKLATRKVSESTGTASFLQELEEWYTWLDNALVLDALMRVANEESEH
NQASIVFPPPGASEENGLPHTSARTQLPQSMKIMHEIMYKLEVLYVLCVLLMGRQRNQVHRMIAEFKLIPGLNNLFDKLIWRKHSASALVLHGHNQNCDC
SPDITLKIQFLRLLQSFSDHHENKYLLLNNQELNELSAISLKANIPEVEAVLNTDRSLVCDGKRGLLTRLLQVMKKEPAESSFRFWQARAVESFLRGTTS
YADQMFLLKRGLLEHILYCIVDSECKSRDVLQSYFDLLGELMKFNVDAFKRFNKYINTDAKFQVFLKQINSSLVDSNMLVRCVTLSLDRFENQVDMKVAE
VLSECRLLAYISQVPTQMSFLFRLINIIHVQTLTQENVSCLNTSLVILMLARRKERLPLYLRLLQRMEHSKKYPGFLLNNFHNLLRFWQQHYLHKDKDST
CLENSSCISFSYWKETVSILLNPDRQSPSALVSYIEEPYMDIDRDFTEE*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pcDNA5 FRT/TO GFP NLS TRPC4AP192.01 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU61064
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Compared to reference sequence there is a silent change at S129 (c387t).
• Low Temp: No