cDNA Clone Ubiquilin

• Synonyms: -
• Plasmid: pET28a-6HiS-TEVb-HALO-Ubiquilin M557K L584K tetramer
• Parent Plasmid: pET28a-6HiS-TEVb-HALO
• Expressed: 6HiS-TEVb-HALO-Ubiquilin M557K L584K tetramer
• Genbank: NM_053067.2
• Tag: 6His
• Tag: HALO
• Species: Human

Sequence of Insert

AGATCTGGAGGTGGAGTAAATCCTCAGCTACAGAATCCAGAAGTCAGATT
TCAGCAACAACTGGAACAACTCAGTGCAAAGGGATTTTTGAACCGTGAAG
CAAACTTGCAAGCTCTAATAGCAACAGGAGGTGATATCAATGCAGCTATT
GAAAGGTTAAAGGGCTCCCAGCCATCATAGGGAGGTGGAGGATCC

Amino Acid Sequence

MGSSHHHHHHENLYFQMAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTP
VLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDH
VRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRP
IPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEV
EMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPV
PKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGS
EIARWLSTLEISGGSGGGVNPQLQNPEVRFQQQLEQLSAKGFLNREANLQ
ALIATGGDINAAIERLKGSQPSGGGGSGGGVNPQLQNPEVRFQQQLEQLS
AKGFLNREANLQALIATGGDINAAIERLKGSQPSGGGGSGGGVNPQLQNP
EVRFQQQLEQLSAKGFLNREANLQALIATGGDINAAIERLKGSQPSGGGG
SGGGVNPQLQNPEVRFQQQLEQLSAKGFLNREANLQALIATGGDINAAIE
RLKGSQPS*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pET28a-6HiS-TEVb-HALO-Ubiquilin M557K L584K tetramer 158.25 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU29835
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Ubiquilin UBA domain M557K L584K tetramer (flanked by Gly linkers).; Parental 23713.
• Low Temp: No