cDNA Clone DET1

• Plasmid: pcDNA5 FRT/TO GFP NLS DET1
• Parent Plasmid: pcDNA5 FRT/TO GFP NLS
• Expressed: GFP NLS DET1
• Genbank: NM_017996.4
• Tag: GFP
• Species: Human

Sequence of Insert

AGATCTATGGTGGGACAGATACTAAAAAGAGATGTGATAATGGATCATCATGTTTCTACCATCAAGCCTCGAAGAATCCAAAACCAAAATGTCATTCACCGCTTGGAACGCCGGCGGATCAGTTCAGGCAAGGCAGGTACCCACTGGCACCAAGTCCGAGTGTTCCATCAGAATGTCTTCCCCAACTTCACAGTTGTCAACGTTGAAAAGCCTCCTTGTTTCTTGCGTAAATTCTCACCTGATGGACGCTACTTTATTGCTTTTTCTTCAGACCAGACATCTCTTGAAATCTATGAGTACCAGGGCTGCCAGGCAGCAGAGGACCTACTGCAGGGATACGAAGGAGAAATCCTGTCCAATGGCAATGACCAGCGGTCAGTGAATATCCGGGGCCGGCTCTTTGAACGCTTTTTTGTCCTGCTGCACATTACCAATGTTGCGGCCAATGGTGAGCACCTGAACCGGGAGTGTAGTCTCTTCACTGATGACTGCCGCTGTGTCATCGTGGGCTCAGCTGCCTACCTCCCAGATGAGCCTCACCCTCCATTTTTTGAGGTATATCGGAACAGTGAATCAGTGACCCCCAACCCACGGTCCCCTCTAGAAGACTATTCCCTCCATATCATTGACCTTCACACCGGCCGCTTATGTGATACACGCACGTTCAAGTGTGACAAGGTGGTCTTGTCACACAACCAAGGGCTGTACTTGTACAAAAACATCCTGGCCATCTTGTCTGTGCAACAACAGACCATCCATGTCTTCCAGGTGACTCCTGAAGGCACTTTCATTGATGTGCGGACCATTGGCCGCTTTTGCTATGAGGATGACCTGCTCACTGTGTCAGCTGTTTTCCCTGAGGTACAGCGGGACAGTCAGACAGGCATGGCCAATCCCTTTAGGGATCCTTTCATCAATTCCCTCAAACACCGGTTGCTGGTATATTTGTGGCGCCGGGCAGAACAGGATGGTAGTGCAATGGCCAAGAGGCGCTTCTTCCAGTATTTTGACCAACTGCGGCAGCTGCGAATGTGGAAAATGCAGCTTCTGGATGAAAACCACCTGTTTATCAAGTACACTAGTGAGGATGTAGTAACACTGCGAGTCACAGATCCATCACAGGCATCTTTCTTTGTGGTGTACAATATGGTGACGACAGAGGTGATTGCTGTGTTTGAGAATACATCAGATGAGCTTTTGGAGCTCTTTGAGAACTTCTGTGACCTTTTTCGTAATGCTACCCTGCACAGTGAAGTTCAGTTTCCCTGCTCAGCTTCTAGCAACAATTTTGCAAGGCAGATCCAGCGCCGGTTCAAAGACACTATTATAAATGCCAAGTATGGAGGGCACACAGAGGCAGTACGCCGGCTGCTGGGTCAGCTCCCCATCAGTGCTCAGTCTTACAGCGGTAGCCCCTATCTGGATTTGTCTCTCTTCAGTTATGATGACAAGTGGGTATCTGTCATGGAGCGGCCCAAGACTTGTGGAGATCACCCAATCAGGTTCTATGCCCGGGACTCGGGCCTGCTCAAGTTTGAGATCCAGGCGGGGTTATTGGGCCGCCCCATCAACCACACAGTGCGACGCCTTGTTGCCTTCACCTTTCACCCTTTTGAGCCTTTCGCTATTTCTGTGCAGAGGACTAATGCTGAGTATGTTGTCAACTTCCATATGCGACACTGCTGCACGTAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLPPKKKRKVGSMVGQILKRDVIMDHHVSTIKPRRIQNQNVIHRLERRRISSGKAGTHWH
QVRVFHQNVFPNFTVVNVEKPPCFLRKFSPDGRYFIAFSSDQTSLEIYEYQGCQAAEDLLQGYEGEILSNGNDQRSVNIRGRLFERFFVLLHITNVAANG
EHLNRECSLFTDDCRCVIVGSAAYLPDEPHPPFFEVYRNSESVTPNPRSPLEDYSLHIIDLHTGRLCDTRTFKCDKVVLSHNQGLYLYKNILAILSVQQQ
TIHVFQVTPEGTFIDVRTIGRFCYEDDLLTVSAVFPEVQRDSQTGMANPFRDPFINSLKHRLLVYLWRRAEQDGSAMAKRRFFQYFDQLRQLRMWKMQLL
DENHLFIKYTSEDVVTLRVTDPSQASFFVVYNMVTTEVIAVFENTSDELLELFENFCDLFRNATLHSEVQFPCSASSNNFARQIQRRFKDTIINAKYGGH
TEAVRRLLGQLPISAQSYSGSPYLDLSLFSYDDKWVSVMERPKTCGDHPIRFYARDSGLLKFEIQAGLLGRPINHTVRRLVAFTFHPFEPFAISVQRTNA
EYVVNFHMRHCCT*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pcDNA5 FRT/TO GFP NLS DET1315.21 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU63625
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Low Temp: No