cDNA Clone Exo5

• Synonyms: C1orf176; DEM1; Exo V; hExo5
• Plasmid: pGEX6P1 TEV 3C Exo5 R344Afster10
• Parent Plasmid: pGEX6P1
• Expressed: GST TEV 3C Exo5 R344Afster10
• Genbank: NM_022774.1 GI:12232456 with coding change G172V: EST-derived and common variant
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCAGAGACAAGAGAAGAGGAGACAGTGTCAGCAGAAGCCTCAGGGTTCTCAGACTTGAGTGACTCAGAGTTCCTGGAGTTTCTGGACCTAGAAGATGCCCAAGAGTCAAAGGCTTTAGTTAACATGCCTGGCCCATCTTCTGAATCCCTTGGGAAGGATGACAAACCCATAAGCTTACAAAACTGGAAAAGAGGATTGGATATATTATCACCCATGGAGAGATTCCACCTTAAATATTTATATGTCACTGACCTGGCTACTCAGAACTGGTGTGAACTGCAAACAGCATATGGGAAGGAGCTTCCTGGTTTCTTGGCACCTGAGAAGGCAGCTGTGTTGGACACTGGTGCCAGCATACACCTAGCTAGAGAACTAGAACTTCATGATCTTGTGACTGTCCCAGTCACCACTAAAGAAGATGCTTGGGCAATTAAGTTTCTGAACATACTTTTGCTGATTCCTACCCTGCAGTCAGAAGGGCACATCAGAGAGTTTCCAGTGTTTGGGGAAGTGGAGGGTGTACTTCTTGTTGGAGTGATTGATGAGCTGCACTATACAGCCAAGGGGGAACTGGAGCTGGCGGAACTCAAGACACGCAGGCGCCCTATGCTCCCTCTGGAAGCTCAGAAGAAGAAAGACTGTTTTCAAGTCAGCCTATACAAATATATCTTTGATGCCATGGTACAAGGAAAAGTGACCCCTGCTAGCCTAATCCACCACACAAAGTTGTGTCTAGAAAAGCCACTGGGGCCATCAGTGCTGAGGCATGCCCAGCAGGGAGGCTTCTCTGTGAAGTCTTTGGGTGACCTCATGGAACTTGTCTTCTTGTCTCTAACACTGTCAGACCTCCCAGTTATTGATATCTTGAAGATTGAGTATATCCACCAAGAGACTGCCACTGTGCTGGGTACTGAGATTGTAGCCTTCAAAGAGAAGGAGGTGAGAGCCAAGGTGCAGCATTATATGGCCTACTGGATGGGCCACCGAGAGCCCCAGGGAGTTGACGTGGAGGAGGCTTGGAAGTGCGCGGACGTGTACCTATGCAGACATTTGTGAGTGGAGAAAGGGCAGTGGAGTGCTCAGCTCTACACTGGCGCCCCAAGTCAAAAAAGCCAAATGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDENLYFQGGLEVLFQGPLGSMAETREEETVSAEASGFSDLSDSEFLEFLDLEDAQESKALVNMPGPSSESLGKDDKPISLQ
NWKRGLDILSPMERFHLKYLYVTDLATQNWCELQTAYGKELPGFLAPEKAAVLDTGASIHLARELELHDLVTVPVTTKEDAWAIKFLNILLLIPTLQSEG
HIREFPVFGEVEGVLLVGVIDELHYTAKGELELAELKTRRRPMLPLEAQKKKDCFQVSLYKYIFDAMVQGKVTPASLIHHTKLCLEKPLGPSVLRHAQQG
GFSVKSLGDLMELVFLSLTLSDLPVIDILKIEYIHQETATVLGTEIVAFKEKEVRAKVQHYMAYWMGHREPQGVDVEEAWKCADVYLCRHL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU63642
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Insertion of g nucleotide after nucleotide 1029 in Exo5 ORF, introduces R344A and causes frameshift resulting in stop codon 10 residues further downstream.
• Low Temp: No