cDNA Clone EAP1/YKL204W

• Plasmid: pRS413 ADH Pr 400-EAP1/YKL204W-200
• Parent Plasmid: pRS413 ADH Pr
• Expressed: EAP1/YKL204W
• Genbank: (ID:853631)

Sequence of Insert

ggatccGTTTTAAAGCAAGGTCAATAGCTTTCAGGCGCAGAAAACCTGAAAATAAAAAAAAAATGCAAAACGAGAGTAAAAGTTGTAATTATATTTATACTAATATTTAAATTGGTAATATGTAATTAGACTTATCATTCACTTTGGTTTAGCAGCTAGTCATGTAATGGCACTAAAAGTGCTTCTTTATCTATTATTTATACGTGACATACCATTACTTCTATGTATTACGTAAAGAATGACAAACGTTTAGTTTTTTTAATTTCAAAGAAATCGTTGGAAGGTTCAAAAAGGATCAGGTGAGATATTAGAGTATATGAATGTTTCAGTCTCTAAGCTACACAAAGAACTAGCATAGTAGGACTTTAACCCATACAATGGAACTCAACGACCCTTCAATTATATCAAGCAGCCAGTTTTCAGGTGAATTGTCTGACAGCGATACTGCAGCCGCTACTCACAAATCGCAACAAGCCATCTCAAATTTATTCCAAAAATTAGCCAAAAAGGGCAGAGAAGAGAAGCCAATCGGTAGCGTAGAATCTTCAACTGATAGCTCTAACATTTCTGTTGCTACGAGCGGTAACAATAAAGAAAGCAATAAAAAAAAAAATAAGAAAACGGCCATGCTAAATTTCAGCAGTTTGACGGACCCTATAACGAACTACAAACCAATGGATCTGCAATACAAGACATATGCATACTCCATGAATGAACTTTATCATTTGAAACCATCTTTGGCAAGTGCTTCGTACGAAGAAGACCCACTTATTTCGGAACTTGTGAGGAGTTTACCCAAAAGAAAGTTTTGGCGATTGCGCATGGGACCTCCAGATCAAAAGCATGCTAATAACCATCATTTTAATGGGAACAATGGTGGAGGCAGTTGGAAGGCTGGTTACAAGAATGGGAAAAACGATGAGAGAAGAATGAGTAGAACCAAAAACATGCAGGGAGGCAAACGAAGATCTCAACAAGATGATGAAGAAAAAAAGATTGACCAAGAAATGCTGGAGATGGACAAGAATCTTCAATTAGGAGGTGACGTCGGCCATTCAATTGCAGATTTTGAAGATTGGAAGGCAAAGATGAAAGAATTAGAATTGAAAAAACTTTCAAAATCTAAAGGAATCAGTAACTCTACCGCAATTGCTCCCAGGGAAAGCGCCTCTCATGAAACACCCACCGATTTAAGACCGGTCATTCCAAGGGGCCCTAGTTCAATAACAGATTTTTTGAATTTAAAGAGGCAAGATAAGAAGGAAGAATCTTCACAACAAACACCTGGTATTCCTGTTGGACAGCCAAGCCTATCCAAAACCAGTATAGAACAAGTTAACGAATTAGAAACTAATTCAGATTTGGGTAAAAGTTCATCATCTCGGTTTTCATCATTTTTCAATAAATCTGCCACTTCGTTGCCTTCATTAGATAATAATAATCAAGTGCCATCGTCAAACGTGTCTGTAGTAAACAACGATGGCAACAGTACACCTCATCAAAGTGGTTCAAGACTGATGTCGTTTTTCAAAGAATCTAGATCGAGCACTCCAAATGCAGAATCGCAGTTGTTATCAGCCTCAGACAAAGATAATGGAAAGATGCAGACTCTTCCGCAATTCCAGCAGCAACCGCAACAAATGCAACCAATGGCATTTACCCAGCATCCTCCTAATAACAACGCATTTTTCAATGGGCTATTGAATAAAGGTAAAAGTGAAACTAGTACTCCTCCTCCTCCACCCCCAGGACTAATTGCTCATCAGGGTCCACAATTTCCTGTGATGGGTGTACCACCAAATTTTCCACAACGCATGATGCCACCGCCACCAGGTCTCGTTCAATTCCAGAAAGATTCTAAAGATGTAAATAAGAAGGAAGACAGGCAATTAAGGCAAAATAAAAATCCAAACGGAACAAGAAATAGCAAGGGAAAACAAGAGGAAACAGCAACGCCAGATCTGCCTCAACAGCAATACATGCCACCACCCCCACCTCCAGGGTTTTTTCCAATGCATCCTAACTTTCCTAACGGCCCAATGCCACCATTACCACAGGGGTTCCCAATCCCACCAAATGGTATGTTACCGGTAACAGGACAACAGCCACAGCCACCTTATCCAAATATGATGCTACAAGGAAATTTCCCCCCTAATTTCCAGCAAGGTTTTGGTAGTAACTCCCCTATGCCAATTCCATCTATTATTAATGCCAATGGCAAAAATGTTACAAACCAGTTACCACCAGGATTAAACTCTAAAAAGAATATAAAATGAGATGCGTAACGAGCGAGTACTTGACAGGAAACGACAAAAACACAGTGAACATACTATCTAAAAGCATGTTCTTTTCCTTTTCATTTTTCATCTTTATTATCTAGAGTTTGTATATAACATGTATCCTATATTATCTTATCCTATCATCATCACTTAACTTGTTATAATTCTCCCATATACTTATGTTTTCGCCTTTGATAgtcgac

Amino Acid Sequence

MELNDPSIISSSQFSGELSDSDTAAATHKSQQAISNLFQKLAKKGREEKPIGSVESSTDSSNISVATSGNNKESNKKKNKKTAMLNFSSLTDPITNYKPMDLQYKTYAYSMNELYHLKPSLASASYEEDPLISELVRSLPKRKFWRLRMGPPDQKHANNHHFNGNNGGGSWKAGYKNGKNDERRMSRTKNMQGGKRRSQQDDEEKKIDQEMLEMDKNLQLGGDVGHSIADFEDWKAKMKELELKKLSKSKGISNSTAIAPRESASHETPTDLRPVIPRGPSSITDFLNLKRQDKKEESSQQTPGIPVGQPSLSKTSIEQVNELETNSDLGKSSSSRFSSFFNKSATSLPSLDNNNQVPSSNVSVVNNDGNSTPHQSGSRLMSFFKESRSSTPNAESQLLSASDKDNGKMQTLPQFQQQPQQMQPMAFTQHPPNNNAFFNGLLNKGKSETSTPPPPPPGLIAHQGPQFPVMGVPPNFPQRMMPPPPGLVQFQKDSKDVNKKEDRQLRQNKNPNGTRNSKGKQEETATPDLPQQQYMPPPPPPGFFPMHPNFPNGPMPPLPQGFPIPPNGMLPVTGQQPQPPYPNMMLQGNFPPNFQQGFGSNSPMPIPSIINANGKNVTNQLPPGLNSKKNIK*

• Vector Type: Yeast
• Antibiotic: Amp
• DU Number: DU59804
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Cloning of EAP1 including 400bp of the 5'UTR and 200bp of the 3'UTR in the vector pRS413-ADH-Pr
• Low Temp: No