cDNA Clone DOA1/YKL213C

• Plasmid: pRS413 ADH Pr 400-DOA1/YKL213C-200
• Parent Plasmid: pRS413 ADH Pr
• Expressed: DOA1/YKL213C
• Genbank: ID:853667

Sequence of Insert

gtcgacATCGTTACTGTTATAGGGATATGGTTTCTAATCCTTTCTCCTATTTCCTTATCTAAACCTGTGGTATTTTTCCCTTGCCTTTTCAACTTCAATAAGCTGGAATTAAATTTGTAGTGTGGGTAAGCAGTAGTGCAGCAATGCGTGCGTGTGCAAGCTCTTGAATATTGAAGGTCTACAGGTTACTAAAACAATAGGAGCGAGGCTTGGACGTCGAGAAAGTTGCTGACCTTGGCATTTCCTTTGCGAGTTTTTCAAAATTTTTCACACTCGTTCAGGACCACGAAAAATAAATCATATGAGATTTTAGTACGCTGATAATAAGGGAAGGCCACTGTCTTGGCTTATTTTATATATATATATTCATGTGTGATAGTAAGGTGTAGAGCAGCAGATTTGGAGTATGGGATATCAATTGAGTGCAACACTTAAAGGGCACGATCAGGACGTCAGGGATGTGGTAGCTGTGGATGATTCAAAGGTTGCTAGTGTTTCGAGGGATGGAACAGTTCGCTTGTGGTCTAAAGATGACCAATGGCTCGGTACGGTAGTTTATACAGGACAAGGATTTTTAAATAGTGTGTGTTATGATTCCGAGAAGGAGTTGTTGCTTTTTGGAGGTAAGGATACTATGATCAACGGTGTGCCCTTGTTTGCCACCTCAGGAGAGGATCCCTTGTATACCTTGATTGGCCACCAAGGGAACGTTTGTAGTTTATCCTTCCAAGATGGTGTTGTGATAAGCGGTAGTTGGGACAAAACCGCAAAGGTTTGGAAAGAAGGATCGTTGGTTTACAATTTGCAAGCACACAATGCCTCTGTATGGGATGCCAAAGTCGTATCCTTCTCAGAAAATAAATTCTTGACAGCTTCCGCCGATAAGACGATTAAACTGTGGCAAAACGATAAAGTGATCAAAACTTTCAGTGGGATTCACAATGATGTAGTAAGACATTTAGCTGTGGTGGATGATGGCCATTTTATCAGTTGTTCAAACGATGGCCTCATCAAGTTGGTTGATATGCATACAGGTGACGTACTAAGAACATATGAAGGCCATGAAAGCTTTGTTTATTGCATTAAACTGCTGCCCAATGGAGATATTGTAAGCTGCGGCGAAGACCGAACAGTCCGTATATGGTCAAAGGAAAATGGTTCGTTAAAGCAGGTAATTACACTTCCTGCCATCTCTATTTGGTCCGTTGATTGTATGTCTAATGGTGATATTATTGTGGGAAGTAGTGATAATCTTGTGAGAATATTTTCGCAAGAGAAGTCAAGATGGGCTTCCGAGGATGAAATAAATGAACTATCTACGCAAGTGGAAAAATCCACTATAAGTTCCAAGACAATAGAATTTGATGAGTCTAAACTATCACCTTATGAAATTTTACAATCGCCGGGGCGCAAAGAGGGTCAAATTGTGGTAGTCAAATCACCTCAAGGTACTATAGAAGCTCATCAATTTTCAAATTCTTCATGGAAAAAGGTTGGTGATGTTGTCGGTGCAGGTGCTACTGGAAACGACAAAAAAATAGAATTCGAGGGTAAAACGTATGACTACGTATTTGATGTGGATATTGAAGATGGAAAACCTCCCTTGAAATTGCCGATTAATGTCAGCGACAACCCATATACAGCAGCCGACAATTTTTTGGCTCGTTATGAACTGCCAATGAGTTATAGGGATCAAGTAGTACAGTTCATACTGAAGAACACTAATGGCATATCGCTAGACCAGCCCAACGATAATGCATCATCTTCTGCAGTATCACCTTCAAAAACGAGCGTAATGAAGGTTCTTCCTGTCAAGCAGTATCTTATCATGGAAAATTATAACCCAGATACTATTTTCAATGGAATAGTAAAAATAAATTCAAATGAGAAGACCTTTGATGATGAAATTCTGGCGCAAATTGGTGGCGCACTACATGACATAGATGAAAGCTGGGAGTTGCTACTTTCATTTGCTAACACTATAAGATCTAACTGGGAAATTAAGACTCCTGCATACGACATAGTACGATTGATTGTAAAAAAATTACCTTATTCTTCAGATATAAAAGATTACATCGAAGAAGGCTTGGGCAATAAGAATATCACTCTAACCATGTTAACTGTGCGTATTTTGGTAAATTGCTTCAATAATGAAAACTGGGGTGTGAAATTACTGGAATCAAATCAAGTTTATAAATCAATTTTTGAGACTATAGATACTGAATTTTCTCAGGCATCTGCTAAACAGTCACAGAATTTGGCTATTGCAGTTTCCACACTAATTTTCAACTATTCAGCTCTCGTCACGAAAGGGAACTCTGATTTAGAACTTTTGCCTATCGTAGCTGACGCCATCAACACCAAGTATGGGCCATTGGAAGAGTATCAAGAATGCGAAGAAGCCGCATATAGGCTAACCGTAGCTTACGGAAATTTGGCTACTGTTGAACCTACATTAAGACAATTTGCCAACTCCGTAACTTGGCTTGCAAACATCAAAAGGAGCTATGGGAACGTGCCAAGGTTTAAGGATATTTTCGACGATCTCTCCTAATACTTTTACAGCAATCATATAAAACACATAATGTCTAGATAGCAAATAATCTTTAAAATTCTTTCTGCCCTATTGAAAATTATGACAAACTATCACGGTCATTTTCTGTCTAACTCGGCAAGAAATTATCATCGTGAAATGAAAAAGGTTACATACAATAAGGTTATTTTGAGTACAGACTGATAAATAGATGGAAAAGActcgag

Amino Acid Sequence

MGYQLSATLKGHDQDVRDVVAVDDSKVASVSRDGTVRLWSKDDQWLGTVVYTGQGFLNSVCYDSEKELLLFGGKDTMINGVPLFATSGEDPLYTLIGHQGNVCSLSFQDGVVISGSWDKTAKVWKEGSLVYNLQAHNASVWDAKVVSFSENKFLTASADKTIKLWQNDKVIKTFSGIHNDVVRHLAVVDDGHFISCSNDGLIKLVDMHTGDVLRTYEGHESFVYCIKLLPNGDIVSCGEDRTVRIWSKENGSLKQVITLPAISIWSVDCMSNGDIIVGSSDNLVRIFSQEKSRWASEDEINELSTQVEKSTISSKTIEFDESKLSPYEILQSPGRKEGQIVVVKSPQGTIEAHQFSNSSWKKVGDVVGAGATGNDKKIEFEGKTYDYVFDVDIEDGKPPLKLPINVSDNPYTAADNFLARYELPMSYRDQVVQFILKNTNGISLDQPNDNASSSAVSPSKTSVMKVLPVKQYLIMENYNPDTIFNGIVKINSNEKTFDDEILAQIGGALHDIDESWELLLSFANTIRSNWEIKTPAYDIVRLIVKKLPYSSDIKDYIEEGLGNKNITLTMLTVRILVNCFNNENWGVKLLESNQVYKSIFETIDTEFSQASAKQSQNLAIAVSTLIFNYSALVTKGNSDLELLPIVADAINTKYGPLEEYQECEEAAYRLTVAYGNLATVEPTLRQFANSVTWLANIKRSYGNVPRFKDIFDDLS*

• Vector Type: Yeast
• Antibiotic: Amp
• DU Number: DU59817
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Cloning of DOA1 including 400bp at the 5'UTR and 200bp at the 3'UTR in the vector pRS413-ADH-Pr
• Low Temp: No