cDNA Clone PKC iota [PRKCI]

• Synonyms: protein kinase C iota
• Plasmid: pFB HTb -PRKCI
• Parent Plasmid: pFB HTb
• Expressed: 6His-PRKCI
• Genbank: NM_002740.5 → NP_002731.4
• Tag: 6His
• Species: Human

Sequence of Insert

ggatccATGCCGACCCAGAGGGACAGCAGCACCATGTCCCACACGGTCGC
AGGCGGCGGCAGCGGGGACCATTCCCACCAGGTCCGGGTGAAAGCCTACT
ACCGCGGGGATATCATGATAACACATTTTGAACCTTCCATCTCCTTTGAG
GGCCTTTGCAATGAGGTTCGAGACATGTGTTCTTTTGACAACGAACAGCT
CTTCACCATGAAATGGATAGATGAGGAAGGAGACCCGTGTACAGTATCAT
CTCAGTTGGAGTTAGAAGAAGCCTTTAGACTTTATGAGCTAAACAAGGAT
TCTGAACTCTTGATTCATGTGTTCCCTTGTGTACCAGAACGTCCTGGGAT
GCCTTGTCCAGGAGAAGATAAATCCATCTACCGTAGAGGTGCACGCCGCT
GGAGAAAGCTTTATTGTGCCAATGGCCACACTTTCCAAGCCAAGCGTTTC
AACAGGCGTGCTCACTGTGCCATCTGCACAGACCGAATATGGGGACTTGG
ACGCCAAGGATATAAGTGCATCAACTGCAAACTCTTGGTTCATAAGAAGT
GCCATAAACTCGTCACAATTGAATGTGGGCGGCATTCTTTGCCACAGGAA
CCAGTGATGCCCATGGATCAGTCATCCATGCATTCTGACCATGCACAGAC
AGTAATTCCATATAATCCTTCAAGTCATGAGAGTTTGGATCAAGTTGGTG
AAGAAAAAGAGGCAATGAACACCAGGGAAAGTGGCAAAGCTTCATCCAGT
CTAGGTCTTCAGGATTTTGATTTGCTCCGGGTAATAGGAAGAGGAAGTTA
TGCCAAAGTACTGTTGGTTCGATTAAAAAAAACAGATCGTATTTATGCAA
TGAAAGTTGTGAAAAAAGAGCTTGTTAATGATGATGAGGATATTGATTGG
GTACAGACAGAGAAGCATGTGTTTGAGCAGGCATCCAATCATCCTTTCCT
TGTTGGGCTGCATTCTTGCTTTCAGACAGAAAGCAGATTGTTCTTTGTTA
TAGAGTATGTAAATGGAGGAGACCTAATGTTTCATATGCAGCGACAAAGA
AAACTTCCTGAAGAACATGCCAGATTTTACTCTGCAGAAATCAGTCTAGC
ATTAAATTATCTTCATGAGCGAGGGATAATTTATAGAGATTTGAAACTGG
ACAATGTATTACTGGACTCTGAAGGCCACATTAAACTCACTGACTACGGC
ATGTGTAAGGAAGGATTACGGCCAGGAGATACAACCAGCACTTTCTGTGG
TACTCCTAATTACATTGCTCCTGAAATTTTAAGAGGAGAAGATTATGGTT
TCAGTGTTGACTGGTGGGCTCTTGGAGTGCTCATGTTTGAGATGATGGCA
GGAAGGTCTCCATTTGATATTGTTGGGAGCTCCGATAACCCTGACCAGAA
CACAGAGGATTATCTCTTCCAAGTTATTTTGGAAAAACAAATTCGCATAC
CACGTTCTCTGTCTGTAAAAGCTGCAAGTGTTCTGAAGAGTTTTCTTAAT
AAGGACCCTAAGGAACGATTGGGTTGTCATCCTCAAACAGGATTTGCTGA
TATTCAGGGACACCCGTTCTTCCGAAATGTTGATTGGGATATGATGGAGC
AAAAACAGGTGGTACCTCCCTTTAAACCAAATATTTCTGGGGAATTTGGT
TTGGACAACTTTGATTCTCAGTTTACTAATGAACCTGTCCAGCTCACTCC
AGATGACGATGACATTGTGAGGAAGATTGATCAGTCTGAATTTGAAGGTT
TTGAGTATATCAATCCTCTTTTGATGTCTGCAGAAGAATGTGTCTGAgcg
gccgc

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMPTQRDSSTMSHTVAGGGSGDH
SHQVRVKAYYRGDIMITHFEPSISFEGLCNEVRDMCSFDNEQLFTMKWID
EEGDPCTVSSQLELEEAFRLYELNKDSELLIHVFPCVPERPGMPCPGEDK
SIYRRGARRWRKLYCANGHTFQAKRFNRRAHCAICTDRIWGLGRQGYKCI
NCKLLVHKKCHKLVTIECGRHSLPQEPVMPMDQSSMHSDHAQTVIPYNPS
SHESLDQVGEEKEAMNTRESGKASSSLGLQDFDLLRVIGRGSYAKVLLVR
LKKTDRIYAMKVVKKELVNDDEDIDWVQTEKHVFEQASNHPFLVGLHSCF
QTESRLFFVIEYVNGGDLMFHMQRQRKLPEEHARFYSAEISLALNYLHER
GIIYRDLKLDNVLLDSEGHIKLTDYGMCKEGLRPGDTTSTFCGTPNYIAP
EILRGEDYGFSVDWWALGVLMFEMMAGRSPFDIVGSSDNPDQNTEDYLFQ
VILEKQIRIPRSLSVKAASVLKSFLNKDPKERLGCHPQTGFADIQGHPFF
RNVDWDMMEQKQVVPPFKPNISGEFGLDNFDSQFTNEPVQLTPDDDDIVR
KIDQSEFEGFEYINPLLMSAEECV*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU29919
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Low Temp: No