cDNA Clone DCAF1

• Synonyms: RIP; VPRBP
• Plasmid: pcDNA5D FRT/TO GFP DCAF1
• Parent Plasmid: pcDNA5D FRT/TO GFP
• Expressed: GFP-DCAF1
• Genbank: NM_014703.2 with delta A87 (seen in isoform 2)
• Tag: GFP
• Species: Human

Sequence of Insert

GCGGCCGCGATGACTACAGTAGTGGTACATGTGGACTCCAAAGCTGAGCTCACTACCCTGCTGGAGCAGTGGGAAAAGGAACATGGCAGTGGGCAGGACATGGTACCTATCCTTACCAGGATGTCTCAATTGATTGAAAAAGAAACTGAAGAGTATCGTAAAGGGGATCCAGACCCATTTGATGATCGACATCCTGGTCGAGCTGATCCAGAGTGTATGCTGGGCCACTTGCTGAGAATACTCTTCAAGAATGATGATTTCATGAATCTGGTGAATGCATATGTGATGACAAGCCGAGAGCCCCCTTTAAACACTGCAGCTTGCAGACTCCTATTAGACATCATGCCAGGGCTGGAAACTGCTGTCGTCTTTCAAGAAAAGGAGGGAATTGTCGAGAATCTTTTCAAATGGGCCCGAGAGGCCGATCAACCATTGAGGACATATTCTACTGGACTGTTAGGAGGTGCTATGGAAAATCAAGACATTGCTGCCAACTATAGAGATGAAAATTCACAGCTGGTGGCAATAGTGCTTCGAAGACTGAGGGAGCTACAGCTACAGGAAGTGGCTTTGCGGCAGGAAAACAAGCGTCCCAGTCCACGGAAGCTCTCTTCTGAACCCCTTTTGCCTCTGGATGAGGAGGCTGTGGATATGGACTATGGTGACATGGCTGTAGATGTAGTGGATGGAGACCAAGAGGAAGCTTCTGGAGACATGGAGATCTCCTTTCATCTTGATTCAGGCCACAAGACTAGTAGCAGAGTGAACTCAACAACCAAACCTGAGGATGGAGGATTAAAGAAAAACAAGTCAGCAAAACAGGGTGACAGAGAGAACTTTAGGAAAGCCAAGCAAAAGTTGGGTTTCTCATCTTCTGATCCAGATCGCATGTTTGTTGAGCTGTCTAATAGCAGTTGGTCAGAAATGTCTCCCTGGGTGATTGGCACCAATTATACCCTTTATCCTATGACTCCTGCTATCGAGCAGCGACTCATTCTCCAATATTTGACCCCTCTAGGAGAATATCAGGAGCTACTTCCCATATTCATGCAACTTGGATCACGGGAGCTGATGATGTTCTATATTGACCTGAAGCAAACTAATGATGTCCTGCTTACATTTGAGGCACTAAAGCACCTAGCATCTCTCCTGCTCCATAACAAATTTGCCACAGAATTTGTTGCGCATGGTGGAGTACAGAAATTACTGGAAATACCTCGTCCTTCTATGGCTGCAACCGGTGTGTCTATGTGTTTGTATTACCTATCCTACAATCAGGATGCCATGGAAAGAGTTTGCATGCATCCCCACAATGTTCTGTCTGATGTGGTGAACTATACCCTGTGGTTAATGGAGTGTTCTCATGCTTCAGGATGCTGCCATGCTACCATGTTTTTTTCAATTTGCTTCTCATTTCGGGCCGTCTTGGAGCTCTTTGACCGCTATGATGGTCTTCGTCGTCTGGTGAACTTGATCAGTACTTTGGAGATTCTAAATTTGGAAGATCAGGGTGCACTTCTGAGTGATGATGAAATATTTGCTAGCCGCCAAACTGGGAAACATACCTGCATGGCCTTGCGCAAATACTTTGAGGCTCACCTGGCCATTAAATTGGAACAAGTGAAGCAGTCACTTCAGAGGACTGAGGGTGGCATTCTTGTCCACCCACAACCCCCGTACAAGGCATGCTCATATACTCATGAACAGATTGTGGAAATGATGGAATTTTTGATAGAATATGGCCCAGCGCAGCTATATTGGGAACCAGCTGAAGTTTTCCTCAAACTTTCTTGTGTGCAACTCTTGTTGCAGCTTATTTCTATTGCCTGCAATTGGAAGACCTATTATGCAAGGAATGACACTGTGCGCTTTGCTTTGGATGTCCTGGCTATTCTTACTGTGGTGCCAAAAATCCAGCTCCAGTTGGCAGAATCAGTGGACGTGTTGGATGAGGCTGGATCTACAGTCTCTACTGTAGGTATCAGCATTATTTTGGGAGTGGCTGAGGGTGAGTTCTTCATCCATGATGCTGAAATTCAGAAGTCAGCACTTCAGATTATCATCAATTGTGTGTGTGGCCCAGATAACCGAATATCCAGTATTGGTAAATTTATCTCTGGTACTCCTCGGAGAAAGCTGCCTCAGAACCCTAAAAGCAGTGAGCACACCCTGGCCAAGATGTGGAATGTGGTTCAGTCCAACAACGGCATCAAGGTGCTCCTGTCCTTACTGTCCATTAAGATGCCCATCACAGATGCAGACCAAATCCGGGCCCTGGCCTGCAAAGCCCTAGTGGGCCTGTCTCGCAGTAGCACTGTCCGGCAGATCATCAGTAAACTGCCCCTTTTCAGCAGCTGCCAGATCCAGCAGCTGATGAAGGAGCCTGTGCTGCAGGACAAGCGCAGTGACCATGTCAAGTTCTGCAAGTATGCTGCTGAACTCATTGAACGGGTGTCAGGAAAACCACTTCTCATTGGCACTGATGTTTCCCTAGCACGACTGCAGAAAGCAGATGTTGTTGCCCAGTCAAGGATCTCCTTCCCTGAGAAAGAGCTGCTTTTGTTGATACGAAACCATCTTATTTCTAAAGGGCTTGGAGAAACAGCAACCGTGCTGACAAAAGAGGCTGACCTGCCCATGACTGCTGCCTCCCATTCTTCTGCCTTTACCCCAGTCACTGCTGCTGCTTCTCCTGTCTCTCTACCCCGAACCCCTCGTATCGCTAATGGCATTGCAACTCGTCTGGGCAGCCATGCTGCTGTGGGTGCCTCTGCGCCTTCTGCCCCTACTGCTCATCCTCAGCCACGGCCCCCCCAGGGTCCGCTAGCTCTGCCCGGCCCATCTTATGCAGGCAACTCCCCTTTGATTGGTAGAATCAGTTTTATCAGAGAGAGGCCATCACCCTGCAATGGCAGGAAAATCAGAGTGTTGCGGCAGAAGTCGGACCATGGTGCCTACAGCCAAAGCCCAGCCATAAAAAAACAGCTGGACAGACATCTTCCTTCCCCACCTACGCTGGACAGTATAATCACAGAGTATCTTAGAGAACAACATGCTCGCTGCAAGAATCCAGTTGCCACCTGCCCACCTTTCTCCCTCTTTACTCCTCACCAATGTCCTGAGCCAAAACAGAGGCGGCAAGCGCCAATAAACTTTACGTCAAGGCTAAACCGCAGGGCATCATTTCCAAAGTATGGAGGGGTGGATGGCGGATGCTTTGATAGGCACCTTATCTTTAGCAGATTCCGTCCTATTTCAGTGTTCCGGGAAGCCAATGAAGATGAGAGTGGCTTCACCTGCTGTGCATTCTCAGCACGGGAGCGGTTCCTGATGCTTGGCACCTGCACAGGGCAGCTGAAGCTCTATAATGTGTTTAGTGGACAGGAGGAGGCCAGCTATAACTGTCACAACTCAGCCATCACACATCTTGAACCTTCCAGGGATGGGTCCTTGCTGCTGACATCTGCTACTTGGAGCCAGCCTTTGTCTGCACTTTGGGGAATGAAGTCAGTATTTGATATGAAGCATTCCTTCACAGAAGATCACTATGTTGAGTTCAGTAAGCACTCCCAGGATCGGGTCATCGGCACAAAAGGAGACATTGCCCACATTTATGATATTCAGACTGGCAACAAGCTGTTGACTCTGTTTAACCCAGATCTTGCCAACAACTACAAGAGGAACTGTGCCACCTTTAATCCTACAGATGATCTTGTCTTAAATGATGGCGTCCTCTGGGATGTCCGCTCTGCACAGGCCATCCACAAGTTTGACAAGTTCAATATGAACATCAGTGGTGTTTTCCATCCAAATGGACTGGAGGTGATCATTAATACTGAGATTTGGGACCTTCGAACTTTTCATCTTTTGCATACTGTTCCCGCTCTGGATCAGTGTCGCGTGGTGTTCAATCACACGGGAACAGTGATGTATGGAGCTATGTTGCAGGCAGATGATGAAGATGACTTAATGGAAGAGAGGATGAAAAGCCCCTTTGGGTCATCCTTCCGAACATTTAATGCAACTGACTACAAACCTATAGCAACCATTGATGTGAAACGGAACATCTTTGACCTGTGTACAGACACCAAAGACTGCTATCTTGCTGTCATTGAGAATCAAGGCAGCATGGATGCCCTGAACATGGACACAGTATGCAGGCTGTATGAAGTGGGCAGGCAGCGTCTGGCAGAGGATGAGGATGAAGAGGAGGACCAGGAAGAGGAAGAACAGGAGGAAGAAGATGATGATGAAGATGATGATGACACCGATGATTTAGATGAGCTTGACACTGACCAGTTGCTGGAGGCGGAGTTGGAGGAGGACGACAATAATGAGAACGCAGGGGAAGATGGGGACAATGACTTCTCTCCCTCTGATGAGGAGCTAGCAAACCTTCTAGAGGAGGGAGAGGACGGGGAGGATGAAGACTCTGATGCAGATGAGGAGGTGGAACTGATCCTGGGGGACACTGACAGCTCTGACAACTCTGATTTGGAAGATGACATCATCTTATCTCTGAATGAGTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSPGEFASLEKLAAAMTTVVVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSQLIE
KETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNLVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLR
TYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVALRQENKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGDQEEASGDMEISFHLD
SGHKTSSRVNSTTKPEDGGLKKNKSAKQGDRENFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELL
PIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNY
TLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQ
RTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESV
DVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQNPKSSEHTLAKMWNVVQSNNGIKVLLSLLSI
KMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRIS
FPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGASAPSAPTAHPQPRPPQGPLALPG
PSYAGNSPLIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRR
QAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPS
RDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLW
DVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLMEERMKSPFGSSFRTFNATDYKP
IATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNE
NAGEDGDNDFSPSDEELANLLEEGEDGEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU63705
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Low Temp: No