cDNA Clone CALCOCO1

• Synonyms: calcium binding and coiled-coil domain 1, calphoglin, PP13275, Cocoa
• Plasmid: pBabe.puro-DU C-Term GFP-CALCOCO1 isoform 1
• Parent Plasmid: pBabe.puro-DU C-Term GFP
• Expressed: CALCOCO1-GFP
• Genbank: NM_020898.2
• Tag: GFP
• Species: Human

Sequence of Insert

ggatccATGGAAGAATCACCACTAAGCCGGGCACCATCCCGTGGTGGAGT
CAACTTTCTCAATGTAGCCCGGACCTACATCCCCAACACCAAGGTGGAAT
GTCACTACACCCTTCCCCCAGGCACCATGCCCAGTGCCAGTGACTGGATT
GGCATCTTCAAGGTGGAGGCTGCCTGTGTTCGGGATTACCACACATTTGT
GTGGTCTTCCGTGCCTGAAAGTACAACTGATGGTTCCCCCATTCACACCA
GTGTCCAGTTCCAAGCCAGCTACCTGCCCAAACCAGGAGCTCAGCTCTAC
CAGTTCCGATATGTGAACCGCCAGGGCCAGGTGTGTGGGCAGAGCCCCCC
TTTCCAGTTCCGAGAGCCAAGGCCCATGGATGAACTGGTGACCCTGGAGG
AGGCTGATGGGGGCTCTGACATCCTGCTGGTTGTCCCCAAGGCAACTGTG
TTACAGAACCAGCTCGATGAGAGCCAGCAAGAACGGAATGACCTGATGCA
GCTGAAGCTACAGCTGGAGGGACAGGTGACAGAGCTGAGGAGCCGAGTAC
AGGAGCTCGAGAGGGCTCTGGCAACTGCCAGGCAGGAGCACACGGAGCTG
ATGGAACAGTACAAGGGGATTTCCCGGTCCCATGGGGAGATCACAGAAGA
GAGGGACATCCTGAGCCGGCAACAGGGAGACCATGTGGCACGCATCCTGG
AGCTAGAGGATGACATCCAGACCATCAGTGAGAAAGTGCTGACGAAGGAA
GTGGAGCTGGACAGGCTTAGAGACACAGTGAAGGCCCTGACTCGGGAACA
AGAGAAGCTCCTTGGGCAACTGAAAGAAGTACAAGCAGACAAGGAGCAAA
GTGAGGCTGAGCTCCAAGTGGCACAACAGGAGAACCATCACTTAAATTTG
GACCTGAAGGAGGCGAAGAGCTGGCAAGAGGAGCAGAGTGCTCAGGCTCA
GCGACTGAAAGACAAGGTGGCCCAGATGAAGGACACCCTAGGCCAGGCCC
AGCAGCGGGTGGCCGAGCTGGAGCCCTTGAAGGAGCAGCTTCGAGGGGCC
CAGGAGCTTGCAGCCTCAAGCCAGCAGAAAGCCACCCTTCTTGGGGAGGA
GTTGGCCAGCGCAGCAGCAGCCAGGGACCGCACCATAGCCGAACTACACC
GCAGCCGCCTGGAAGTGGCTGAAGTTAACGGCAGGCTGGCTGAGCTCGGT
TTGCACTTGAAGGAAGAAAAATGCCAATGGAGCAAGGAGCGGGCAGGGCT
GCTGCAGAGTGTGGAGGCAGAGAAGGACAAGATCCTGAAGCTGAGTGCAG
AGATACTTCGATTGGAGAAGGCAGTTCAGGAGGAGAGGACCCAAAACCAA
GTGTTCAAGACTGAGCTGGCCCGGGAGAAGGATTCTAGCCTGGTACAGTT
GTCAGAAAGTAAGCGGGAGCTGACAGAGCTGCGGTCAGCCCTGCGTGTGC
TCCAGAAGGAAAAGGAGCAGTTACAGGAGGAGAAACAGGAATTGCTAGAG
TACATGAGAAAGCTAGAGGCCCGCCTGGAGAAGGTGGCAGATGAGAAGTG
GAATGAGGATGCCACCACAGAGGATGAGGAGGCCGCTGTGGGGCTGAGCT
GCCCGGCAGCTCTGACAGACTCAGAGGACGAGTCCCCAGAAGACATGAGG
CTCCCACCCTATGGCCTTTGTGAGCGTGGAGACCCAGGCTCCTCTCCTGC
TGGGCCTCGAGAGGCTTCTCCCCTTGTTGTCATCAGCCAGCCGGCTCCCA
TTTCTCCTCACCTCTCTGGGCCAGCTGAGGACAGTAGCTCTGACTCGGAG
GCTGAAGATGAGAAGTCAGTCCTGATGGCAGCTGTGCAGAGTGGGGGTGA
GGAGGCCAACTTACTGCTTCCTGAACTGGGCAGTGCCTTCTATGACATGG
CCAGTGGCTTTACAGTGGGTACCCTGTCAGAAACCAGCACTGGGGGCCCT
GCCACCCCCACATGGAAGGAGTGTCCTATCTGTAAGGAGCGCTTTCCTGC
TGAGAGTGACAAGGATGCCCTGGAGGACCACATGGATGGACACTTCTTTT
TCAGCACCCAGGACCCCTTCACCTTTGAGgcggccgc

Amino Acid Sequence

MEESPLSRAPSRGGVNFLNVARTYIPNTKVECHYTLPPGTMPSASDWIGI
FKVEAACVRDYHTFVWSSVPESTTDGSPIHTSVQFQASYLPKPGAQLYQF
RYVNRQGQVCGQSPPFQFREPRPMDELVTLEEADGGSDILLVVPKATVLQ
NQLDESQQERNDLMQLKLQLEGQVTELRSRVQELERALATARQEHTELME
QYKGISRSHGEITEERDILSRQQGDHVARILELEDDIQTISEKVLTKEVE
LDRLRDTVKALTREQEKLLGQLKEVQADKEQSEAELQVAQQENHHLNLDL
KEAKSWQEEQSAQAQRLKDKVAQMKDTLGQAQQRVAELEPLKEQLRGAQE
LAASSQQKATLLGEELASAAAARDRTIAELHRSRLEVAEVNGRLAELGLH
LKEEKCQWSKERAGLLQSVEAEKDKILKLSAEILRLEKAVQEERTQNQVF
KTELAREKDSSLVQLSESKRELTELRSALRVLQKEKEQLQEEKQELLEYM
RKLEARLEKVADEKWNEDATTEDEEAAVGLSCPAALTDSEDESPEDMRLP
PYGLCERGDPGSSPAGPREASPLVVISQPAPISPHLSGPAEDSSSDSEAE
DEKSVLMAAVQSGGEEANLLLPELGSAFYDMASGFTVGTLSETSTGGPAT
PTWKECPICKERFPAESDKDALEDHMDGHFFFSTQDPFTFEAAAMVSKGE
ELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPV
PWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMAD
KQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQS
ALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU29941
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 40432; Silent mutations at V181 (GTG>GTA) and S368 (AGT>AGC); Isoform 1
• Low Temp: No