cDNA Clone FAM83D (short variant)

• Synonyms: C20orf129, CHICA, dJ616B8.3
• Plasmid: pGEX FAM83D 1-320
• Parent Plasmid: pGEX 6P-1
• Expressed: GST-3C-FAM83D(M1-Q320)
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCTCTGCTGTCCGAGGGCCTGGACGAGGTGCCCGCCGCCTGCCTGTCGCCGTGCGGGCCGCCCAACCCGACCGAGCTGTTCAGCGAGTCACGGCGCCTGGCTCTGGAGGAGCTGGTGGCGGGCGGCCCCGAAGCCTTCGCGGCCTTCCTGCGACGCGAGCGCCTGGCTCGTTTCCTGAACCCCGATGAGGTGCACGCCATTCTGCGCGCGGCGGAGAGGCCGGGAGAGGAGGGCGCGGCGGCGGCGGCGGCGGCCGAGGACTCGTTCGGCTCCTCGCACGACTGCTCTTCGGGCACCTACTTCCCCGAGCAGTCGGACCTGGAGCCACCGCTGTTGGAGCTTGGCTGGCCCGCCTTCTACCAGGGCGCCTACCGCGGCGCCACGCGTGTCGAGACGCACTTCCAGCCCCGCGGCGCTGGCGAAGGTGGCCCCTACGGCTGCAAGGACGCTCTGCGCCAGCAGCTCCGCTCGGCGCGAGAGGTGATTGCAGTGGTCATGGACGTGTTCACAGACATCGACATCTTCAGAGACCTGCAAGAAATATGCAGGAAACAGGGAGTTGCTGTGTATATCCTTCTGGACCAGGCTCTCCTCTCTCAATTTCTGGATATGTGCATGGATCTGAAAGTTCATCCTGAACAGGAAAAGTTAATGACAGTTCGGACTATCACAGGAAATATCTACTATGCAAGGTCAGGAACTAAGATTATTGGGAAGGTTCACGAAAAGTTCACGTTGATTGATGGCATCCGCGTGGCAACAGGCTCCTACAGTTTTACATGGACGGATGGCAAATTAAACAGCAGTAACTTGGTAATTCTGTCTGGCCAAGTGGTTGAACACTTTGATCTGGAATTCCGAATCCTGTATGCCCAGTCCAAGCCCATCAGCCCCAAACTCCTGTCTCACTTCCAGAGCAGCAACAAGTTTGATCACCTCACCAACCGAAAACCACAGTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMALLSEGLDEVPAACLSPCGPPNPTELFSESRRLALEELVAGGPEAFAAFLRRERLARFLNPDEVHAIL
RAAERPGEEGAAAAAAAEDSFGSSHDCSSGTYFPEQSDLEPPLLELGWPAFYQGAYRGATRVETHFQPRGAGEGGPYGCKDALRQQLRSAREVIAVVMDV
FTDIDIFRDLQEICRKQGVAVYILLDQALLSQFLDMCMDLKVHPEQEKLMTVRTITGNIYYARSGTKIIGKVHEKFTLIDGIRVATGSYSFTWTDGKLNS
SNLVILSGQVVEHFDLEFRILYAQSKPISPKLLSHFQSSNKFDHLTNRKPQ*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU64334
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Hi Tom, Please could you clone an N-term GST-precision site tag onto the n-term of FAM83D (M1-Q320) in a pGEX vector? Nikki made the mammalian GFP-tag version of this already (DU29202) which may be useful for sub cloning. Many thanks, Best wishes, Luke.
• Low Temp: No