cDNA Clone DENND4C

• Plasmid: pET15b 6HIS SUMO DENND4C L32-T672
• Parent Plasmid: pET15b 6His SUMO
• Expressed: 6HIS SUMO DENND4C L32-T672
• Genbank: NM_017925.6
• Tag: GFP
• Species: Human

Sequence of Insert

ATGTTAGATACTAAGTCAACTGGACCTAAAGCTCCAATTACAGACATTGCCATTATTATCAAATCAGCTGGAGAAACAGTACCTGAAGGTTACACCTGTGTAGAAGCCACTCCATCAGCTCTCCAAGCAAACTTGAACTATGGAAGTCTGAAAAGCCCAGAACTTTTCCTCTGTTATAAGAGAGGGAGAGATAAACCACCGCTTACAGATATTGGAGTTCTATATGAAGGGAAAGAACGGCTTATTCCAGGATGTGAAGTGATCCTAGCCACACCCTATGGTCGCTGTGCCAATGTCAACAATAGTTCAACTACTTCACAAAGAATCTTTATCACTTATCGAAGGGCTCCTCCAGTTCGACCCCAGAATTCCTTGGCTGTAACTGATATCTGTGTTATTGTAACCAGTAAAGGAGAAACTCCTCCTCATACCTTCTGCAAAGTTGACAAAAACTTAAATTGTGGAATGTGGGGTTCCAGCGTGTTTCTGTGTTATAAGAAGTCTGTACCTGCTTCAAATGCAATAGCATATAAGGCTGGTTTAATTTTTAGATATCCAGAAGAGGACTATGAGTCATTTCCACTCTCAGAATCAGATGTACCTCTTTTCTGCCTTCCTATGGGAGCTACTATTGAGTGCTGGGATCCTGAAACCAAATATCCACTTCCAGTTTTTTCAACTTTTGTCTTGACAGGTTCTTCAGCCAAAAAGGTATATGGAGCTGCCATTCAGTTTTATGAACCTTACTCTCGGGAACTTCTATCAGAGAAACAGCTTATGCACCTGGGCTTGTTGACGCCTGTGGAGAGAAAAATGGTCTCCAAATCCATCAATACAAACAAATGCATTTGTTTACTCTCACACTGGCCTTTTTTTGAAGCTTTTAGGAAATTTCTTATGTTTATCTACAAACTTTCTGTGTCTGGACCACATCCTCTTCCCATTGAAAAGCACATTTCACATTTTATGCAAAACATCCCTTTTCCTTCACCACAAAGACCGAGAATCCTTGTCCAGCTGTCAGTCCATGATGCATTAATATTATCACAGCCAGTTTCTACACCTTTACCACTAAGTGGAGCCAACTTTAGCACCTTGCTAATGAATCTGGGTCCTGAGAATTGTGCAACACTGCTGCTCTTTGTTTTACTTGAGAGTAAAATTCTGCTGCATTCTCTTAGGCCAGCTGTCTTGACTGGGGTAGCTGAAGCTGTTGTAGCTATGATCTTTCCATTTCAGTGGCAATGCCCATATATTCCCCTTTGTCCTCTTTCACTGGCTGCAGTGCTTAGTGCACCTTTACCATTTATAGTTGGAGTTGACTCAAGGTATTTTGATCTTCATGACCCACCACAAGATGTTGTTTGCATTGACTTGGATACGAACATGTTATATGTATCAGATGAAAAGAAGAACATGAACTGGAAGCAACTTCCCAAAAAGCCGTGCAAAAATCTACTTAGCACCTTAAAGAAATTGTATCCCCAGCTGTCTTCAGTTCACCAAAAAACTCAAGAAGGCTCAGCGATTGACATGACTCCAATTGAAGCAGATTTCTCCTGGCAAAAGAAGATGACACAGCTTGAGATGGAAATTCAAGAGGCATTTTTGCGCTTTATGGCGTCTATTTTAAAAGGATATAGAACATATCTCAGACCAATCACAGAGGCTCCTTCAAATAAAGCCACAGCTGCTGATTCATTGTTTGACCGACAGGGATTTTTAAAAAGTCGAGATCGTGCCTATGCAAAATTCTATACCCTTTTATCCAAAACACAGATTTTTATTCGTTTCATTGAAGAATGCAGTTTTGTAAGTGATAAAGATACTGGATTAGCATTTTTTGATGACTGCATAGAAAAGTTGTTTCCTGATAAAGGCACAGAGAAAACAGATAAGGTTGATTTTGATTCAGCAGAAGATACCTGAGCGGCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGMLDTKSTGPKAPITDIAIIIKSAGETVPEGYTCVEATPSALQANLNYGSLKSPELFLCYKRGRDKPPLTDIGVLYEGKERLIPGCEVILATPYGRCANVNNSSTTSQRIFITYRRAPPVRPQNSLAVTDICVIVTSKGETPPHTFCKVDKNLNCGMWGSSVFLCYKKSVPASNAIAYKAGLIFRYPEEDYESFPLSESDVPLFCLPMGATIECWDPETKYPLPVFSTFVLTGSSAKKVYGAAIQFYEPYSRELLSEKQLMHLGLLTPVERKMVSKSINTNKCICLLSHWPFFEAFRKFLMFIYKLSVSGPHPLPIEKHISHFMQNIPFPSPQRPRILVQLSVHDALILSQPVSTPLPLSGANFSTLLMNLGPENCATLLLFVLLESKILLHSLRPAVLTGVAEAVVAMIFPFQWQCPYIPLCPLSLAAVLSAPLPFIVGVDSRYFDLHDPPQDVVCIDLDTNMLYVSDEKKNMNWKQLPKKPCKNLLSTLKKLYPQLSSVHQKTQEGSAIDMTPIEADFSWQKKMTQLEMEIQEAFLRFMASILKGYRTYLRPITEAPSNKATAADSLFDRQGFLKSRDRAYAKFYTLLSKTQIFIRFIEECSFVSDKDTGLAFFDDCIEKLFPDKGTEKTDKVDFDSAEDT*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU62781
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Linker after SENP cleavage deleted making clone flush
• Low Temp: No