cDNA Clone Zuo1

• Plasmid: pRS413-ADH-Pr 400-ZUO1/YGR285C -200 S55E
• Parent Plasmid: pRS413-ADH-Pr
• Expressed: ZUO1/YGR285C S50E

Sequence of Insert

GGATCCGCCTTGTACTTTCAACCGTTCATTTCATTAAGAACTTGACTAAATATGAACATTTCTTAAAAAAAAGGTTGACATATAAAAATAATCGAATATAAACGATGGAATTTTTATAAAATTAAACACATATATATATATATATTAACTATAAATATGTCAAAGAAACCATACAATCATAGATTTATAACTATCTTTTGGATGACATTAATGAACATAACGCTCCTAATACAAATGTCCAAAAAATATTACCCGCAAATACGAATCTTTTTTTTTTCTCGATGAAATTTTGCAAAGAGTTCGAAATTTTTATTTCAAGAGCTGGTAGAGAAAATTTCATAAGGTTTTCCTACCGATGCTTTTATAAAATCTTCGTTTTGTCTCACATATACCAACAAGAGTAACGATGTTTTCTTTACCTACCCTAACCTCAGACATCACTGTTGAAGTCAACAGTTCCGCTACCAAAACCCCATTCGTCCGTCGTCCGGTCGAACCGGTTGGTAAGTTCTTTTTGCAACATGCTCAAAGAACTTTGAGAAACCACACCTGGGAGGAATTTGAAAGAATTGAAGCTGAAAAGAACGTCAAAACCGTTGATGAATCCAATGTCGACCCAGATGAGTTGTTATTCGACACTGAATTGGCCGATGAAGATTTACTGACTCATGATGCTAGAGACTGGAAAACTGCCGATTTGTATGCTGCTATGGGTTTGTCTAAGTTGCGTTTCAGAGCTACTGAAAGTCAAATCATCAAGGCTCACAGAAAACAAGTTGTCAAGTACCATCCAGACAAGCAATCTGCTGCTGGTGGTAGTTTGGACCAAGATGGCTTTTTCAAGATTATTCAAAAGGCCTTTGAAACTTTGACTGATTCCAACAAGAGAGCTCAGTACGACTCATGTGATTTTGTTGCCGATGTTCCTCCTCCAAAGAAGGGTACCGATTATGACTTTTATGAAGCTTGGGGCCCCGTTTTCGAAGCTGAAGCTCGTTTTTCTAAGAAGACTCCTATTCCTTCTCTAGGTAACAAAGATTCTTCCAAGAAGGAAGTTGAACAATTCTATGCTTTCTGGCACAGATTTGACTCCTGGAGAACCTTTGAGTTCTTGGACGAAGATGTCCCAGATGACTCTTCTAACAGAGACCACAAGCGTTACATTGAAAGAAAGAACAAGGCCGCAAGAGACAAGAAGAAGACTGCTGATAACGCTAGATTGGTCAAACTTGTTGAAAGAGCTGTCAGTGAAGATCCCCGTATCAAAATGTTCAAAGAAGAAGAGAAGAAGGAAAAGGAAAGAAGAAAATGGGAAAGAGAAGCCGGTGCCAGAGCTGAAGCTGAAGCTAAGGCCAAGGCCGAAGCTGAAGCGAAGGCTAAAGCTGAATCTGAAGCCAAGGCTAACGCCTCCGCAAAAGCTGACAAAAAGAAGGCTAAGGAAGCTGCTAAGGCCGCCAAGAAAAAGAACAAGAGAGCCATCCGTAACTCTGCTAAGGAAGCTGACTACTTTGGTGATGCTGACAAGGCCACCACGATTGACGAACAAGTTGGTTTGATCGTTGACAGTTTGAATGACGAAGAGTTAGTGTCCACCGCCGATAAGATCAAGGCCAATGCTGCTGGTGCCAAGGAAGTTTTGAAGGAATCTGCAAAGACTATTGTCGATTCTGGCAAACTACCATCCAGCTTGTTGTCCTACTTCGTGTGAATACCGTAAGAAATGGAATAGAATATATACGAATGTATACGAATATTATAGAGAACGTTCTCTTTTATTTCTATAATGAATAGGTTCGGGTAACGGTTCCCTTTTTAGGTATTTCTAGAAGATGAGAGAAGAGGGAATAATGAGAAAGGCGAAAAATAAAGGACACCTTTAACGAAAGATCAAAGGTGTCCTTATTTACCTCGAG

Amino Acid Sequence

MFSLPTLTSDITVEVNSSATKTPFVRRPVEPVGKFFLQHAQRTLRNHTWEEFERIEAEKNVKTVDESNVDPDELLFDTELADEDLLTHDARDWKTADLYAAMGLSKLRFRATESQIIKAHRKQVVKYHPDKQSAAGGSLDQDGFFKIIQKAFETLTDSNKRAQYDSCDFVADVPPPKKGTDYDFYEAWGPVFEAEARFSKKTPIPSLGNKDSSKKEVEQFYAFWHRFDSWRTFEFLDEDVPDDSSNRDHKRYIERKNKAARDKKKTADNARLVKLVERAVSEDPRIKMFKEEEKKEKERRKWEREAGARAEAEAKAKAEAEAKAKAESEAKANASAKADKKKAKEAAKAAKKKNKRAIRNSAKEADYFGDADKATTIDEQVGLIVDSLNDEELVSTADKIKANAAGAKEVLKESAKTIVDSGKLPSSLLSYFV*

• Vector Type: Yeast
• DU Number: DU66119
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Zuo1 S50E mutation in PRS413 Zuo1 (DU59842)
• Low Temp: No