cDNA Clone ZUO1/YGR285C

• Plasmid: pRS413-ADH-Pr 400-ZUO1/YGR285C -200 S50A
• Parent Plasmid: pRS413-ADH-Pr
• Expressed: ZUO1/YGR285C S50A
• Genbank: ID:853202

Sequence of Insert

GGATCCGCCTTGTACTTTCAACCGTTCATTTCATTAAGAACTTGACTAAATATGAACATTTCTTAAAAAAAAGGTTGACATATAAAAATAATCGAATATAAACGATGGAATTTTTATAAAATTAAACACATATATATATATATATTAACTATAAATATGTCAAAGAAACCATACAATCATAGATTTATAACTATCTTTTGGATGACATTAATGAACATAACGCTCCTAATACAAATGTCCAAAAAATATTACCCGCAAATACGAATCTTTTTTTTTTCTCGATGAAATTTTGCAAAGAGTTCGAAATTTTTATTTCAAGAGCTGGTAGAGAAAATTTCATAAGGTTTTCCTACCGATGCTTTTATAAAATCTTCGTTTTGTCTCACATATACCAACAAGAGTAACGATGTTTTCTTTACCTACCCTAACCTCAGACATCACTGTTGAAGTCAACAGTTCCGCTACCAAAACCCCATTCGTCCGTCGTCCGGTCGAACCGGTTGGTAAGTTCTTTTTGCAACATGCTCAAAGAACTTTGAGAAACCACACCTGGGCTGAATTTGAAAGAATTGAAGCTGAAAAGAACGTCAAAACCGTTGATGAATCCAATGTCGACCCAGATGAGTTGTTATTCGACACTGAATTGGCCGATGAAGATTTACTGACTCATGATGCTAGAGACTGGAAAACTGCCGATTTGTATGCTGCTATGGGTTTGTCTAAGTTGCGTTTCAGAGCTACTGAAAGTCAAATCATCAAGGCTCACAGAAAACAAGTTGTCAAGTACCATCCAGACAAGCAATCTGCTGCTGGTGGTAGTTTGGACCAAGATGGCTTTTTCAAGATTATTCAAAAGGCCTTTGAAACTTTGACTGATTCCAACAAGAGAGCTCAGTACGACTCATGTGATTTTGTTGCCGATGTTCCTCCTCCAAAGAAGGGTACCGATTATGACTTTTATGAAGCTTGGGGCCCCGTTTTCGAAGCTGAAGCTCGTTTTTCTAAGAAGACTCCTATTCCTTCTCTAGGTAACAAAGATTCTTCCAAGAAGGAAGTTGAACAATTCTATGCTTTCTGGCACAGATTTGACTCCTGGAGAACCTTTGAGTTCTTGGACGAAGATGTCCCAGATGACTCTTCTAACAGAGACCACAAGCGTTACATTGAAAGAAAGAACAAGGCCGCAAGAGACAAGAAGAAGACTGCTGATAACGCTAGATTGGTCAAACTTGTTGAAAGAGCTGTCAGTGAAGATCCCCGTATCAAAATGTTCAAAGAAGAAGAGAAGAAGGAAAAGGAAAGAAGAAAATGGGAAAGAGAAGCCGGTGCCAGAGCTGAAGCTGAAGCTAAGGCCAAGGCCGAAGCTGAAGCGAAGGCTAAAGCTGAATCTGAAGCCAAGGCTAACGCCTCCGCAAAAGCTGACAAAAAGAAGGCTAAGGAAGCTGCTAAGGCCGCCAAGAAAAAGAACAAGAGAGCCATCCGTAACTCTGCTAAGGAAGCTGACTACTTTGGTGATGCTGACAAGGCCACCACGATTGACGAACAAGTTGGTTTGATCGTTGACAGTTTGAATGACGAAGAGTTAGTGTCCACCGCCGATAAGATCAAGGCCAATGCTGCTGGTGCCAAGGAAGTTTTGAAGGAATCTGCAAAGACTATTGTCGATTCTGGCAAACTACCATCCAGCTTGTTGTCCTACTTCGTGTGAATACCGTAAGAAATGGAATAGAATATATACGAATGTATACGAATATTATAGAGAACGTTCTCTTTTATTTCTATAATGAATAGGTTCGGGTAACGGTTCCCTTTTTAGGTATTTCTAGAAGATGAGAGAAGAGGGAATAATGAGAAAGGCGAAAAATAAAGGACACCTTTAACGAAAGATCAAAGGTGTCCTTATTTACCTCGAG

Amino Acid Sequence

MFSLPTLTSDITVEVNSSATKTPFVRRPVEPVGKFFLQHAQRTLRNHTWAEFERIEAEKNVKTVDESNVDPDELLFDTELADEDLLTHDARDWKTADLYAAMGLSKLRFRATESQIIKAHRKQVVKYHPDKQSAAGGSLDQDGFFKIIQKAFETLTDSNKRAQYDSCDFVADVPPPKKGTDYDFYEAWGPVFEAEARFSKKTPIPSLGNKDSSKKEVEQFYAFWHRFDSWRTFEFLDEDVPDDSSNRDHKRYIERKNKAARDKKKTADNARLVKLVERAVSEDPRIKMFKEEEKKEKERRKWEREAGARAEAEAKAKAEAEAKAKAESEAKANASAKADKKKAKEAAKAAKKKNKRAIRNSAKEADYFGDADKATTIDEQVGLIVDSLNDEELVSTADKIKANAAGAKEVLKESAKTIVDSGKLPSSLLSYFV*

• Vector Type: Yeast
• Antibiotic: Amp
• DU Number: DU59960
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Cloning of ZUO1 including 400bp of the 5'UTR and 200bp of the 3'UTR in the vector pRS413-ADH-Pr
• Low Temp: No