cDNA Clone Mindy1(KIAA1390)

• Plasmid: pGEX6P1 KIAA1390 V160A 110-384
• Parent Plasmid: pGEX6P1
• Expressed: GST-KIAA1390 V160A 110-384
• Tag: GST

Sequence of Insert

CTCGAGATGGAGCCAGATTTCTACTGTGTCAAGTGGATCCCTTGGAAAGGAGAACAGACACCCATCATCACCCAGAGCACTAACGGCCCTTGCCCTCTCCTTGCCATCATGAACATCCTCTTTCTTCAGTGGAAGGTGAAGCTCCCCCCGCAGAAGGAAGCGATCACATCGGATGAGCTCATGGCCCATCTTGGAAACTGCCTCCTGTCCATCAAGCCCCAGGAGAAGTCAGAGGGACTTCAGCTTAATTTTCAGCAGAATGTGGATGATGCAATGACAGTGCTGCCTAAACTGGCCACAGGTCTGGATGTCAATGTGCGATTCACAGGCGTCTCTGATTTTGAGTATACACCCGAGTGCAGTGTCTTTGACCTGCTAGGCATACCTCTGTACCATGGCTGGCTTGTTGATCCACAGAGTCCTGAGGCTGTGCGTGCAGTTGGGAAACTGAGTTACAACCAGCTGGTGGAGAGGATCATCACCTGCAAACACTCCAGTGACACCAACCTCGTGACAGAAGGCCTGATTGCAGAGCAGTTCCTGGAGACCACCGCGGCCCAGCTGACCTACCACGGACTGTGTGAGCTGACAGCAGCTGCTAAGGAGGGTGAACTTAGCGTCTTTTTCCGAAACAACCACTTTAGCACCATGACTAAGCATAAGAGTCACTTATACCTACTGGTCACTGACCAGGGCTTTCTACAGGAGGAGCAAGTCGTATGGGAGAGCCTGCACAATGTGGATGGAGACAGCTGCTTTTGTGACTCTGACTTTCACCTGAGTCATTCCCTGGGCAAGGGGCCTGGAGCAGAAGGTGGGAGTGGCTCCCCAGAATAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFPGRLEMEPDFYCVKWIPWKGEQTPIITQSTNGPCPLLAIMNILFLQWKVKLPPQKEAITSDELMAHLGNCLLSIKPQEKSEGLQLNFQQNVDDAMTVLPKLATGLDVNVRFTGVSDFEYTPECSVFDLLGIPLYHGWLVDPQSPEAVRAVGKLSYNQLVERIITCKHSSDTNLVTEGLIAEQFLETTAAQLTYHGLCELTAAAKEGELSVFFRNNHFSTMTKHKSHLYLLVTDQGFLQEEQVVWESLHNVDGDSCFCDSDFHLSHSLGKGPGAEGGSGSPE*

• Vector Type: Bacterial
• DU Number: DU59996
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please mutate valine 160 to alanine in 47257
• Low Temp: No