cDNA Clone FAM83D (mouse)

• Plasmid: pBabe.puro-DU C-Term GFP (mu)FAM83D
• Parent Plasmid: pBabe.puro-DU C-Term GFP
• Expressed: (mu)FAM83D-GFP
• Genbank: NM_027975.2
• Tag: GFP
• Species: Mouse

Sequence of Insert

agatctATGGCTGCTCGCTTCGAGCTGTTGGACGACCTTCCTGCGGCCTG
CCTGTCTCCGTGCGGACCGCCCAACCCCACGGAGCTGTTCAGCGAGGCGC
GGCGTCTGGCTCTCGAGCAGCTGCTGGCCGGTGGTCCCGACGCCTGGGCC
GCCTTCTTACGGCGTGAGCGGCTGGGCCGCTTCCTCAACGCCGACGAGGT
GCGCGAGGTTCTGGGCGCTGCCGAGCGGCCGGGCGAGGATGGGGCGGCGG
TAGCGGAGGACTCGTTCGGCTCCTCTCACGAATGCTCGTCGGGCACCTAC
TTTCCGGAGCAGTCGGACCTGGAACCGCCGGCGTTGGAGCTCGGCTGGCC
TTCCTTCTACCAGGGTGCCTACCGCGGTGCCACGCGTGTCGAGGCTCATT
TCCAGCCCCGCGGCGCGGGCGCCGGAGGCCCGTATGGCTGCAAGGATGCG
CTGCGCCAGCAGTTGCGCTCCGCCCGAGAGGTGATTGCTGTGGTAATGGA
CGTTTTCTCAGACATCGACATCTTCCGAGACCTGCAGGAGAGCTGTAGGA
AGCGGGGGGTGGCTGTGTACATCCTCCTGGACCAGACTCTGCTCCCCCAC
TTTTTGGACATGTGCATGGATCTGAGAGTTCATCCTGAGCAGGAAAAGCT
GATGACAGTTCGGACCATTACAGGAAATATCTACTATGCAAGGTCAGGAA
CAAAGGTTGTCGGGAAGGTTCATGAGAAGTTCACACTGATTGACGGAATC
CGGGTGGCAACAGGCTCTTACAGTTTTACCTGGACCGACGGCAAATTAAA
CAGCAGTAACTTGGTAATTCTGTCTGGCCAAGTGGTTGAACACTTTGATC
TGGAGTTCCGGATCCTGTATGCTCAGTCAGAGCCCATCAGCTCCAAACTC
CTGTCCAACTTCCAGATCAATAGCAAGTTTGACCATCTGGCTGACCGAAA
GCCACAGTCGAAGGAGCCCACACTGGGCAATCTGCTGCGAATGAGGCTGG
CCAGGCTCTCAAGTACTCCCAGGAAGAGCAACCTGGGCCCAGAGGAGCCG
CCAAAAGACAGAGCCAAACCCAAGCGCCCTGACTCTGAGGCTTCTACCAT
CAGCGATGAAGACTATTTCCACAGCCACAAGGACCAGCTAGAGGATAGTA
AGGTGGCTGATGCTGCTACCCAAACAGAGCCCAGAGAAGAGATGGCTGCA
GTAAGCCTGAGTGAGGTGGGAACTCAGACTAGTTCCAGCATGATGTGTGT
TGGGACCCAAACCACAGTTGTCACCAGGGCAGCGAGCTCCCAGGCCACAG
TGTGGTCCAAGTCCACTACCACACAGACTGAAGCTGACGAGAGCTTCCTT
CCTCAGGGTGCCCAGTCCAAAGAAGGGTCACCGGCATCCAAGATGTCAGT
GTCGAGGTCTTCCAGTGTGAGGTCATCCTCTTCTGTGTCTTCCCAGGGCT
CGCTGGCAAGCTCGGTGAGCTCCCATGTCTCCCTGACGGCTGCTGATCTC
CACACTCCCGCATACCCCAAGTACCTGGGTCTGGGCACCCCGCATCTGGA
TCTGTGCCTAAGGGACTCGTTCAGAAACTTAAGTAAGGAGCGGCAGGTCC
ACTTCACTGGCATCAGGTCCCGGCTCACCCAGATGCTCACCGTGCTCTCA
CGGAGAACGCTCTTCACAGAGCACTACCTCAGCTACAGCCCCGGGAGCTT
TACCAGAGCCTCTACCAACCTGGTTTCTGTGAGGGACATAGCCCTTTACC
CTCCCTATCAGgcggccgc

Amino Acid Sequence

MAARFELLDDLPAACLSPCGPPNPTELFSEARRLALEQLLAGGPDAWAAF
LRRERLGRFLNADEVREVLGAAERPGEDGAAVAEDSFGSSHECSSGTYFP
EQSDLEPPALELGWPSFYQGAYRGATRVEAHFQPRGAGAGGPYGCKDALR
QQLRSAREVIAVVMDVFSDIDIFRDLQESCRKRGVAVYILLDQTLLPHFL
DMCMDLRVHPEQEKLMTVRTITGNIYYARSGTKVVGKVHEKFTLIDGIRV
ATGSYSFTWTDGKLNSSNLVILSGQVVEHFDLEFRILYAQSEPISSKLLS
NFQINSKFDHLADRKPQSKEPTLGNLLRMRLARLSSTPRKSNLGPEEPPK
DRAKPKRPDSEASTISDEDYFHSHKDQLEDSKVADAATQTEPREEMAAVS
LSEVGTQTSSSMMCVGTQTTVVTRAASSQATVWSKSTTTQTEADESFLPQ
GAQSKEGSPASKMSVSRSSSVRSSSSVSSQGSLASSVSSHVSLTAADLHT
PAYPKYLGLGTPHLDLCLRDSFRNLSKERQVHFTGIRSRLTQMLTVLSRR
TLFTEHYLSYSPGSFTRASTNLVSVRDIALYPPYQAAAMVSKGEELFTGV
VPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLV
TTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAE
VKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGI
KVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP
NEKRDHMVLLEFVTAAGITLGMDELYK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU65061
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 40432; Silent changes at Pro12 (CCC > CCT) and Thr220 (ACA > ACC)
• Low Temp: No