cDNA Clone DCAF1

• Synonyms: RIP; VPRBP
• Plasmid: pcDNA5D FRT/TO puro mScarlet DCAF1
• Parent Plasmid: pcDNA5D FRT/TO puro mScarlet
• Expressed: mScarlet DCAF1
• Genbank: NM_014703.2 with delta A87 (seen in isoform 2)
• Tag: mScarlet
• Species: Human

Sequence of Insert

GCGGCCGCGATGACTACAGTAGTGGTACATGTGGACTCCAAAGCTGAGCTCACTACCCTGCTGGAGCAGTGGGAAAAGGAACATGGCAGTGGGCAGGACATGGTACCTATCCTTACCAGGATGTCTCAATTGATTGAAAAAGAAACTGAAGAGTATCGTAAAGGGGATCCAGACCCATTTGATGATCGACATCCTGGTCGAGCTGATCCAGAGTGTATGCTGGGCCACTTGCTGAGAATACTCTTCAAGAATGATGATTTCATGAATCTGGTGAATGCATATGTGATGACAAGCCGAGAGCCCCCTTTAAACACTGCAGCTTGCAGACTCCTATTAGACATCATGCCAGGGCTGGAAACTGCTGTCGTCTTTCAAGAAAAGGAGGGAATTGTCGAGAATCTTTTCAAATGGGCCCGAGAGGCCGATCAACCATTGAGGACATATTCTACTGGACTGTTAGGAGGTGCTATGGAAAATCAAGACATTGCTGCCAACTATAGAGATGAAAATTCACAGCTGGTGGCAATAGTGCTTCGAAGACTGAGGGAGCTACAGCTACAGGAAGTGGCTTTGCGGCAGGAAAACAAGCGTCCCAGTCCACGGAAGCTCTCTTCTGAACCCCTTTTGCCTCTGGATGAGGAGGCTGTGGATATGGACTATGGTGACATGGCTGTAGATGTAGTGGATGGAGACCAAGAGGAAGCTTCTGGAGACATGGAGATCTCCTTTCATCTTGATTCAGGCCACAAGACTAGTAGCAGAGTGAACTCAACAACCAAACCTGAGGATGGAGGATTAAAGAAAAACAAGTCAGCAAAACAGGGTGACAGAGAGAACTTTAGGAAAGCCAAGCAAAAGTTGGGTTTCTCATCTTCTGATCCAGATCGCATGTTTGTTGAGCTGTCTAATAGCAGTTGGTCAGAAATGTCTCCCTGGGTGATTGGCACCAATTATACCCTTTATCCTATGACTCCTGCTATCGAGCAGCGACTCATTCTCCAATATTTGACCCCTCTAGGAGAATATCAGGAGCTACTTCCCATATTCATGCAACTTGGATCACGGGAGCTGATGATGTTCTATATTGACCTGAAGCAAACTAATGATGTCCTGCTTACATTTGAGGCACTAAAGCACCTAGCATCTCTCCTGCTCCATAACAAATTTGCCACAGAATTTGTTGCGCATGGTGGAGTACAGAAATTACTGGAAATACCTCGTCCTTCTATGGCTGCAACCGGTGTGTCTATGTGTTTGTATTACCTATCCTACAATCAGGATGCCATGGAAAGAGTTTGCATGCATCCCCACAATGTTCTGTCTGATGTGGTGAACTATACCCTGTGGTTAATGGAGTGTTCTCATGCTTCAGGATGCTGCCATGCTACCATGTTTTTTTCAATTTGCTTCTCATTTCGGGCCGTCTTGGAGCTCTTTGACCGCTATGATGGTCTTCGTCGTCTGGTGAACTTGATCAGTACTTTGGAGATTCTAAATTTGGAAGATCAGGGTGCACTTCTGAGTGATGATGAAATATTTGCTAGCCGCCAAACTGGGAAACATACCTGCATGGCCTTGCGCAAATACTTTGAGGCTCACCTGGCCATTAAATTGGAACAAGTGAAGCAGTCACTTCAGAGGACTGAGGGTGGCATTCTTGTCCACCCACAACCCCCGTACAAGGCATGCTCATATACTCATGAACAGATTGTGGAAATGATGGAATTTTTGATAGAATATGGCCCAGCGCAGCTATATTGGGAACCAGCTGAAGTTTTCCTCAAACTTTCTTGTGTGCAACTCTTGTTGCAGCTTATTTCTATTGCCTGCAATTGGAAGACCTATTATGCAAGGAATGACACTGTGCGCTTTGCTTTGGATGTCCTGGCTATTCTTACTGTGGTGCCAAAAATCCAGCTCCAGTTGGCAGAATCAGTGGACGTGTTGGATGAGGCTGGATCTACAGTCTCTACTGTAGGTATCAGCATTATTTTGGGAGTGGCTGAGGGTGAGTTCTTCATCCATGATGCTGAAATTCAGAAGTCAGCACTTCAGATTATCATCAATTGTGTGTGTGGCCCAGATAACCGAATATCCAGTATTGGTAAATTTATCTCTGGTACTCCTCGGAGAAAGCTGCCTCAGAACCCTAAAAGCAGTGAGCACACCCTGGCCAAGATGTGGAATGTGGTTCAGTCCAACAACGGCATCAAGGTGCTCCTGTCCTTACTGTCCATTAAGATGCCCATCACAGATGCAGACCAAATCCGGGCCCTGGCCTGCAAAGCCCTAGTGGGCCTGTCTCGCAGTAGCACTGTCCGGCAGATCATCAGTAAACTGCCCCTTTTCAGCAGCTGCCAGATCCAGCAGCTGATGAAGGAGCCTGTGCTGCAGGACAAGCGCAGTGACCATGTCAAGTTCTGCAAGTATGCTGCTGAACTCATTGAACGGGTGTCAGGAAAACCACTTCTCATTGGCACTGATGTTTCCCTAGCACGACTGCAGAAAGCAGATGTTGTTGCCCAGTCAAGGATCTCCTTCCCTGAGAAAGAGCTGCTTTTGTTGATACGAAACCATCTTATTTCTAAAGGGCTTGGAGAAACAGCAACCGTGCTGACAAAAGAGGCTGACCTGCCCATGACTGCTGCCTCCCATTCTTCTGCCTTTACCCCAGTCACTGCTGCTGCTTCTCCTGTCTCTCTACCCCGAACCCCTCGTATCGCTAATGGCATTGCAACTCGTCTGGGCAGCCATGCTGCTGTGGGTGCCTCTGCGCCTTCTGCCCCTACTGCTCATCCTCAGCCACGGCCCCCCCAGGGTCCGCTAGCTCTGCCCGGCCCATCTTATGCAGGCAACTCCCCTTTGATTGGTAGAATCAGTTTTATCAGAGAGAGGCCATCACCCTGCAATGGCAGGAAAATCAGAGTGTTGCGGCAGAAGTCGGACCATGGTGCCTACAGCCAAAGCCCAGCCATAAAAAAACAGCTGGACAGACATCTTCCTTCCCCACCTACGCTGGACAGTATAATCACAGAGTATCTTAGAGAACAACATGCTCGCTGCAAGAATCCAGTTGCCACCTGCCCACCTTTCTCCCTCTTTACTCCTCACCAATGTCCTGAGCCAAAACAGAGGCGGCAAGCGCCAATAAACTTTACGTCAAGGCTAAACCGCAGGGCATCATTTCCAAAGTATGGAGGGGTGGATGGCGGATGCTTTGATAGGCACCTTATCTTTAGCAGATTCCGTCCTATTTCAGTGTTCCGGGAAGCCAATGAAGATGAGAGTGGCTTCACCTGCTGTGCATTCTCAGCACGGGAGCGGTTCCTGATGCTTGGCACCTGCACAGGGCAGCTGAAGCTCTATAATGTGTTTAGTGGACAGGAGGAGGCCAGCTATAACTGTCACAACTCAGCCATCACACATCTTGAACCTTCCAGGGATGGGTCCTTGCTGCTGACATCTGCTACTTGGAGCCAGCCTTTGTCTGCACTTTGGGGAATGAAGTCAGTATTTGATATGAAGCATTCCTTCACAGAAGATCACTATGTTGAGTTCAGTAAGCACTCCCAGGATCGGGTCATCGGCACAAAAGGAGACATTGCCCACATTTATGATATTCAGACTGGCAACAAGCTGTTGACTCTGTTTAACCCAGATCTTGCCAACAACTACAAGAGGAACTGTGCCACCTTTAATCCTACAGATGATCTTGTCTTAAATGATGGCGTCCTCTGGGATGTCCGCTCTGCACAGGCCATCCACAAGTTTGACAAGTTCAATATGAACATCAGTGGTGTTTTCCATCCAAATGGACTGGAGGTGATCATTAATACTGAGATTTGGGACCTTCGAACTTTTCATCTTTTGCATACTGTTCCCGCTCTGGATCAGTGTCGCGTGGTGTTCAATCACACGGGAACAGTGATGTATGGAGCTATGTTGCAGGCAGATGATGAAGATGACTTAATGGAAGAGAGGATGAAAAGCCCCTTTGGGTCATCCTTCCGAACATTTAATGCAACTGACTACAAACCTATAGCAACCATTGATGTGAAACGGAACATCTTTGACCTGTGTACAGACACCAAAGACTGCTATCTTGCTGTCATTGAGAATCAAGGCAGCATGGATGCCCTGAACATGGACACAGTATGCAGGCTGTATGAAGTGGGCAGGCAGCGTCTGGCAGAGGATGAGGATGAAGAGGAGGACCAGGAAGAGGAAGAACAGGAGGAAGAAGATGATGATGAAGATGATGATGACACCGATGATTTAGATGAGCTTGACACTGACCAGTTGCTGGAGGCGGAGTTGGAGGAGGACGACAATAATGAGAACGCAGGGGAAGATGGGGACAATGACTTCTCTCCCTCTGATGAGGAGCTAGCAAACCTTCTAGAGGAGGGAGAGGACGGGGAGGATGAAGACTCTGATGCAGATGAGGAGGTGGAACTGATCCTGGGGGACACTGACAGCTCTGACAACTCTGATTTGGAAGATGACATCATCTTATCTCTGAATGAGTGAGCGGCCGC

Amino Acid Sequence

MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNF
EDGGAVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKMALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKL
DITSHNEDYTVVEQYERSEGRHSTGGMDELYKGSPGEFASLEKLAAAMTTVVVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSQLIEKETEEYRKGD
PDPFDDRHPGRADPECMLGHLLRILFKNDDFMNLVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGA
MENQDIAANYRDENSQLVAIVLRRLRELQLQEVALRQENKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGDQEEASGDMEISFHLDSGHKTSSRVN
STTKPEDGGLKKNKSAKQGDRENFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRE
LMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHA
SGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHP
QPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTV
STVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQNPKSSEHTLAKMWNVVQSNNGIKVLLSLLSIKMPITDADQI
RALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLI
RNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGASAPSAPTAHPQPRPPQGPLALPGPSYAGNSPLI
GRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRL
NRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSA
TWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHK
FDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLMEERMKSPFGSSFRTFNATDYKPIATIDVKRNI
FDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDF
SPSDEELANLLEEGEDGEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU63952
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Low Temp: No