cDNA Clone Human NCOA4

• Synonyms: nuclear receptor coactivator 4 isoform 3
• Plasmid: pBabe.hygro-DU-FLAG-NCOA4 Isoform 3 shRNA insensitive
• Parent Plasmid: pBabe.hygro-DU-FLAG
• Expressed: FLAG-NCOA4 Isoform 3 (shRNA insensitive mutant)
• Genbank: NM_001145263.1 → NP_001138735.1
• Tag: FLAG
• Species: Human

Sequence of Insert

ggatccATGAATACCTTCCAAGACCAGAGTGGCAGCTCCAGTAATAGAGA
ACCCCTTTTGAGGTGTAGTGATGCACGGAGGGACTTGGAGCTTGCTATTG
GTGGAGTTCTCCGGGCTGAACAGCAAATTAAAGATAACTTGCGAGAGGTC
AAAGCTCAGATTCACAGTTGCATAAGCCGTCACCTGGAATGTCTTAGAAG
CCGTGAGGTATGGCTGTATGAACAGGTGGACCTTATTTATCAGCTTAAAG
AGGAGACACTTCAACAGCAGGCTCAGCAGCTCTACTCGTTATTGGGCCAG
TTCAATTGTCTTACTCATCAACTGGAGTGTACCCAAAACAAAGATCTAGC
CAATCAAGTCTCTGTGTGCCTGGAGAGACTGGGCAGTTTGACCCTTAAGC
CTGAAGATTCAACTGTCCTGCTCTTTGAAGCTGACACAATTACTCTGCGC
CAGACCATCACCACATTTGGGTCTCTCAAAACCATTCAAATTCCTGAGCA
CTTGATGGCTCATGCTAGTTCAGCAAATATTGGGCCCTTCCTGGAGAAGA
GAGGCTGTATCTCCATGCCAGAGCAGAAGTCAGCATCCGGTATTGTAGCT
GTCCCTTTCAGCGAATGGCTCCTTGGAAGCAAACCTGCCAGTGGTTATCA
AGCTCCTTACATACCCAGCACCGACCCCCAGGACTGGCTTACCCAAAAGC
AGACCTTGGAGAACAGTCAGACTTCTTCCAGAGCCTGCAATTTCTTCAAT
AATGTCGGGGGAAACCTAAAGGGCTTAGAAAACTGGCTCCTCAAGAGTGA
AAAATCAAGTTATCAAAAGTGTAACAGCCATTCCACTACTAGTTCTTTCT
CCATTGAAATGGAAAAGGTTGGAGATCAAGAGCTTCCTGATCAAGATGAG
ATGGACCTATCAGATTGGCTAGTGACTCCCCAGGAATCCCATAAGCTGCG
GAAGCCTGAGAATGGCAGTCGTGAAACCAGTGAGAAGTTTAAGCTCTTAT
TCCAGTCCTATAATGTGAATGATTGGCTTGTCAAGACTGACTCCTGTACC
AACTGTCAGGGAAACCAGCCCAAAGGTGTGGAGATTGAAAACCTGGGCAA
TCTGAAGTGCCTGAATGACCACTTGGAGGCCAAGAAACCATTGTCCACCC
CCAGCATGGTTACAGAGGATTGGCTTGTCCAGAACCATCAGGACCCATGT
AAGGTAGAGGAGGTGTGCAGAGCCAATGAGCCCTGCACAAGCTTTGCAGA
GTGTGTGTGTGATGAGAATTGTGAGAAGGAGGCTCTGTATAAGTGGCTTC
TGAAGAAAGAAGGAAAGGATAAAAATGGGATGCCTGTGGAACCCAAACCT
GAGCCTGAGAAGCATAAAGATTCCCTGAATATGTGGCTCTGTCCTAGAAA
AGAAGTAATAGAACAAACTAAAGCACCAAAGGCAATGACTCCTTCTAGAA
TTGCTGATTCCTTCCAAGTCATAAAGAACAGCCCCTTGTCGGAGTGGCTT
ATCAGGCCCCCATACAAAGAAGGAAGTCCCAAAGAGGTACCGGGAACGGA
GGACAGAGCTGGCAAACAGAAGTTTAAAAGCCCCATGAATACTTCCTGGT
GTTCCTTTAACACAGCTGACTGGGTCCTGCCAGGAAAGAAGATGGGCAAC
CTCAGCCAGTTATCTTCTGGAGAAGACAAGTGGCTGCTTCGAAAGAAGGC
CCAGGAAGTATTACTTAATTCACCTCTACAGGAGGAACATAACTTCCCCC
CAGACCATTATGGCCTCCCTGCAGTTTGTGATCTCTTTGCCTGTATGCAG
CTTAAAGTTGATAAAGAGAAGTGGTTATATCGAACTCCTCTACAGATGTG
Agcggccgc

Amino Acid Sequence

MDYKDDDDKGSMNTFQDQSGSSSNREPLLRCSDARRDLELAIGGVLRAEQ
QIKDNLREVKAQIHSCISRHLECLRSREVWLYEQVDLIYQLKEETLQQQA
QQLYSLLGQFNCLTHQLECTQNKDLANQVSVCLERLGSLTLKPEDSTVLL
FEADTITLRQTITTFGSLKTIQIPEHLMAHASSANIGPFLEKRGCISMPE
QKSASGIVAVPFSEWLLGSKPASGYQAPYIPSTDPQDWLTQKQTLENSQT
SSRACNFFNNVGGNLKGLENWLLKSEKSSYQKCNSHSTTSSFSIEMEKVG
DQELPDQDEMDLSDWLVTPQESHKLRKPENGSRETSEKFKLLFQSYNVND
WLVKTDSCTNCQGNQPKGVEIENLGNLKCLNDHLEAKKPLSTPSMVTEDW
LVQNHQDPCKVEEVCRANEPCTSFAECVCDENCEKEALYKWLLKKEGKDK
NGMPVEPKPEPEKHKDSLNMWLCPRKEVIEQTKAPKAMTPSRIADSFQVI
KNSPLSEWLIRPPYKEGSPKEVPGTEDRAGKQKFKSPMNTSWCSFNTADW
VLPGKKMGNLSQLSSGEDKWLLRKKAQEVLLNSPLQEEHNFPPDHYGLPA
VCDLFACMQLKVDKEKWLYRTPLQM*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU65093
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: PARENTAL 37214; insensitive to shNCOA4 #2 (target sequence: GGAAGUGCCUGGUACUGAA)
• Low Temp: No