cDNA Clone EPS15 tv2

• Plasmid: p416 GPD EPS15 tv2
• Parent Plasmid: p416-GDP
• Expressed: EPS15 tv2
• Genbank: NM_001159969.1

Sequence of Insert

GGATCCATGTATTTAAAAAGCGATTCAGGATTAGGAGGATGGATAACCATACCAGCTGTAGCTGATGTTCTAAAATATTCTTGCATTGTTTGCTGGTCTTCTAGGGAAAAGAATAATGTGGAACAGGACCTTAAGGAGAAGGAAGATACTATTAAACAGAGGACAAGTGAGGTTCAGGATCTTCAAGATGAAGTTCAAAGGGAGAATACTAATCTGCAAAAACTACAGGCCCAGAAACAGCAGGTACAGGAACTCCTTGATGAACTGGATGAGCAGAAAGCCCAGCTGGAGGAGCAACTCAAGGAAGTCAGAAAGAAATGTGCTGAGGAGGCCCAACTGATCTCTTCTCTGAAAGCTGAATTAACTAGTCAGGAATCGCAGATCTCCACTTATGAAGAAGAATTGGCAAAAGCTAGAGAAGAGCTGAGCCGTCTACAGCAAGAAACAGCAGAATTGGAGGAGAGTGTAGAGTCAGGGAAGGCTCAGTTGGAACCTCTTCAGCAGCACCTACAAGATTCACAACAGGAAATTAGTTCAATGCAAATGAAACTGATGGAAATGAAAGATTTGGAAAATCATAATAGTCAGTTAAATTGGTGCAGTAGCCCACACAGCATTCTTGTAAACGGAGCTACAGATTATTGCAGCCTCAGCACCAGCAGCAGTGAAACAGCCAACCTTAATGAACATGTTGAAGGCCAGAGCAACCTAGAGTCTGAGCCCATACACCAGGAATCTCCAGCAAGAAGTAGTCCTGAACTACTGCCTTCTGGTGTGACTGATGAAAATGAGGTGACTACAGCTGTTACTGAAAAAGTTTGTTCTGAACTCGACAATAATAGACATTCAAAAGAGGAAGATCCATTTAATGTAGACTCAAGTTCGCTGACAGGTCCAGTTGCAGATACAAACTTGGATTTTTTCCAGTCTGATCCTTTTGTTGGCAGTGATCCTTTCAAGGATGATCCTTTTGGAAAAATCGATCCATTTGGTGGTGATCCTTTCAAAGGTTCAGATCCATTTGCATCAGACTGTTTCTTCAGGCAATCTACTGATCCTTTTGCCACTTCAAGCACTGACCCTTTCAGTGCAGCCAACAATAGCAGTATTACATCGGTAGAAACGTTGAAGCACAATGATCCTTTTGCTCCTGGTGGAACAGTTGTTGCAGCAAGCGATTCAGCCACAGACCCCTTTGCTTCTGTTTTTGGGAATGAATCATTTGGAGGTGGATTTGCTGACTTCAGCACATTGTCAAAGGTCAACAATGAAGATCCTTTTCGTTCAGCCACATCGAGCTCTGTCAGCAACGTAGTGATTACAAAAAATGTATTTGAGGAAACATCGGTCAAAAGTGAAGATGAACCCCCAGCACTGCCACCAAAGATCGGAACTCCAACAAGACCCTGCCCTCTACCACCTGGGAAAAGATCCATCAACAAATTGGATTCTCCTGATCCCTTTAAACTGAATGATCCATTTCAGCCTTTCCCAGGCAACGATAGCCCCAAAGAAAAAGATCCTGAAATATTTTGTGATCCATTCACTTCTGCTACTACCACTACCAATAAAGAGGCTGATCCAAGCAATTTTGCCAACTTCAGTGCTTATCCCTCTGAAGAAGATATGATCGAATGGGCCAAGAGGGAAAGTGAGAGAGAGGAAGAGCAGAGGCTTGCCCGACTAAATCAGCAGGAACAAGAAGACTTAGAACTGGCTATTGCACTCAGCAAATCTGAGATATCAGAAGCATGAGTCGAC

Amino Acid Sequence

MYLKSDSGLGGWITIPAVADVLKYSCIVCWSSREKNNVEQDLKEKEDTIKQRTSEVQDLQDEVQRENTNLQKLQAQKQQVQELLDELDEQKAQLEEQLKEVRKKCAEEAQLISSLKAELTSQESQISTYEEELAKAREELSRLQQETAELEESVESGKAQLEPLQQHLQDSQQEISSMQMKLMEMKDLENHNSQLNWCSSPHSILVNGATDYCSLSTSSSETANLNEHVEGQSNLESEPIHQESPARSSPELLPSGVTDENEVTTAVTEKVCSELDNNRHSKEEDPFNVDSSSLTGPVADTNLDFFQSDPFVGSDPFKDDPFGKIDPFGGDPFKGSDPFASDCFFRQSTDPFATSSTDPFSAANNSSITSVETLKHNDPFAPGGTVVAASDSATDPFASVFGNESFGGGFADFSTLSKVNNEDPFRSATSSSVSNVVITKNVFEETSVKSEDEPPALPPKIGTPTRPCPLPPGKRSINKLDSPDPFKLNDPFQPFPGNDSPKEKDPEIFCDPFTSATTTTNKEADPSNFANFSAYPSEEDMIEWAKRESEREEEQRLARLNQQEQEDLELAIALSKSEISEA*

• Vector Type: Yeast
• Antibiotic: Amp
• DU Number: DU66191
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Cloning of the human EPS15 (ID: 2060) in the MCS of p416-GPD for expression of EPS15 in yeast under GPD promoter. Silent 387 C-T
• Low Temp: No