cDNA Clone Human NCOA4

• Synonyms: nuclear receptor coactivator 4 isoform 3
• Plasmid: pBabeD hygro-NCOA4-FLAG shRNA insensitive
• Parent Plasmid: pBabeD hygro
• Expressed: NCOA4-FLAG shRNA insensitive mutant
• Genbank: NM_001145263.1 → NP_001138735.1
• Tag: FLAG
• Species: Human

Sequence of Insert

ggatccATGAATACCTTCCAAGACCAGAGTGGCAGCTCCAGTAATAGAGA
ACCCCTTTTGAGGTGTAGTGATGCACGGAGGGACTTGGAGCTTGCTATTG
GTGGAGTTCTCCGGGCTGAACAGCAAATTAAAGATAACTTGCGAGAGGTC
AAAGCTCAGATTCACAGTTGCATAAGCCGTCACCTGGAATGTCTTAGAAG
CCGTGAGGTATGGCTGTATGAACAGGTGGACCTTATTTATCAGCTTAAAG
AGGAGACACTTCAACAGCAGGCTCAGCAGCTCTACTCGTTATTGGGCCAG
TTCAATTGTCTTACTCATCAACTGGAGTGTACCCAAAACAAAGATCTAGC
CAATCAAGTCTCTGTGTGCCTGGAGAGACTGGGCAGTTTGACCCTTAAGC
CTGAAGATTCAACTGTCCTGCTCTTTGAAGCTGACACAATTACTCTGCGC
CAGACCATCACCACATTTGGGTCTCTCAAAACCATTCAAATTCCTGAGCA
CTTGATGGCTCATGCTAGTTCAGCAAATATTGGGCCCTTCCTGGAGAAGA
GAGGCTGTATCTCCATGCCAGAGCAGAAGTCAGCATCCGGTATTGTAGCT
GTCCCTTTCAGCGAATGGCTCCTTGGAAGCAAACCTGCCAGTGGTTATCA
AGCTCCTTACATACCCAGCACCGACCCCCAGGACTGGCTTACCCAAAAGC
AGACCTTGGAGAACAGTCAGACTTCTTCCAGAGCCTGCAATTTCTTCAAT
AATGTCGGGGGAAACCTAAAGGGCTTAGAAAACTGGCTCCTCAAGAGTGA
AAAATCAAGTTATCAAAAGTGTAACAGCCATTCCACTACTAGTTCTTTCT
CCATTGAAATGGAAAAGGTTGGAGATCAAGAGCTTCCTGATCAAGATGAG
ATGGACCTATCAGATTGGCTAGTGACTCCCCAGGAATCCCATAAGCTGCG
GAAGCCTGAGAATGGCAGTCGTGAAACCAGTGAGAAGTTTAAGCTCTTAT
TCCAGTCCTATAATGTGAATGATTGGCTTGTCAAGACTGACTCCTGTACC
AACTGTCAGGGAAACCAGCCCAAAGGTGTGGAGATTGAAAACCTGGGCAA
TCTGAAGTGCCTGAATGACCACTTGGAGGCCAAGAAACCATTGTCCACCC
CCAGCATGGTTACAGAGGATTGGCTTGTCCAGAACCATCAGGACCCATGT
AAGGTAGAGGAGGTGTGCAGAGCCAATGAGCCCTGCACAAGCTTTGCAGA
GTGTGTGTGTGATGAGAATTGTGAGAAGGAGGCTCTGTATAAGTGGCTTC
TGAAGAAAGAAGGAAAGGATAAAAATGGGATGCCTGTGGAACCCAAACCT
GAGCCTGAGAAGCATAAAGATTCCCTGAATATGTGGCTCTGTCCTAGAAA
AGAAGTAATAGAACAAACTAAAGCACCAAAGGCAATGACTCCTTCTAGAA
TTGCTGATTCCTTCCAAGTCATAAAGAACAGCCCCTTGTCGGAGTGGCTT
ATCAGGCCCCCATACAAAGAAGGAAGTCCCAAAGAGGTACCGGGAACGGA
GGACAGAGCTGGCAAACAGAAGTTTAAAAGCCCCATGAATACTTCCTGGT
GTTCCTTTAACACAGCTGACTGGGTCCTGCCAGGAAAGAAGATGGGCAAC
CTCAGCCAGTTATCTTCTGGAGAAGACAAGTGGCTGCTTCGAAAGAAGGC
CCAGGAAGTATTACTTAATTCACCTCTACAGGAGGAACATAACTTCCCCC
CAGACCATTATGGCCTCCCTGCAGTTTGTGATCTCTTTGCCTGTATGCAG
CTTAAAGTTGATAAAGAGAAGTGGTTATATCGAACTCCTCTACAGATGGA
CTACAAGGACGATGACGATAAGTGAGCGGCCGC

Amino Acid Sequence

MNTFQDQSGSSSNREPLLRCSDARRDLELAIGGVLRAEQQIKDNLREVKA
QIHSCISRHLECLRSREVWLYEQVDLIYQLKEETLQQQAQQLYSLLGQFN
CLTHQLECTQNKDLANQVSVCLERLGSLTLKPEDSTVLLFEADTITLRQT
ITTFGSLKTIQIPEHLMAHASSANIGPFLEKRGCISMPEQKSASGIVAVP
FSEWLLGSKPASGYQAPYIPSTDPQDWLTQKQTLENSQTSSRACNFFNNV
GGNLKGLENWLLKSEKSSYQKCNSHSTTSSFSIEMEKVGDQELPDQDEMD
LSDWLVTPQESHKLRKPENGSRETSEKFKLLFQSYNVNDWLVKTDSCTNC
QGNQPKGVEIENLGNLKCLNDHLEAKKPLSTPSMVTEDWLVQNHQDPCKV
EEVCRANEPCTSFAECVCDENCEKEALYKWLLKKEGKDKNGMPVEPKPEP
EKHKDSLNMWLCPRKEVIEQTKAPKAMTPSRIADSFQVIKNSPLSEWLIR
PPYKEGSPKEVPGTEDRAGKQKFKSPMNTSWCSFNTADWVLPGKKMGNLS
QLSSGEDKWLLRKKAQEVLLNSPLQEEHNFPPDHYGLPAVCDLFACMQLK
VDKEKWLYRTPLQMDYKDDDDK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU65142
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Isoform 3 ; Parental 37127; insensitive to shNCOA4 #2 (target sequence: GGAAGUGCCUGGUACUGAA)
• Low Temp: No