cDNA Clone ULK1

• Plasmid: pcDNA5D FRT/TO-GST-2x(6His)-ULK1
• Parent Plasmid: pcDNA5D FRT/TO GST
• Expressed: GST-2x(6His)-ULK1
• Genbank: NM_003565.3
• Tag: GST
• Tag: 6His
• Species: Human

Sequence of Insert

ggatccATGcatcatcatcatcatcatagcagcggcctggtgccgcgcgg
cagccatatggcgagccatcatcatcatcatcatcatcatcatcatggcg
aaaacctgtattttcagggcATGGAGCCCGGCCGCGGCGGCACAGAGACC
GTGGGCAAGTTCGAGTTCTCCCGCAAGGACCTGATCGGCCACGGCGCCTT
CGCGGTGGTCTTCAAGGGCCGCCACCGCGAGAAGCACGATTTGGAGGTCG
CCGTCAAGTGCATTAACAAGAAGAACCTCGCCAAGTCTCAGACGCTGCTG
GGGAAGGAAATCAAAATCCTGAAGGAACTGAAACATGAAAACATCGTGGC
CCTGTACGACTTCCAGGAAATGGCTAATTCTGTCTACCTGGTTATGGAGT
ACTGCAACGGTGGGGACCTGGCCGACTACCTGCACGCCATGCGCACGCTG
AGCGAGGACACCATCAGGCTCTTCCTGCAGCAGATCGCGGGCGCCATGCG
GCTTCTGCACAGCAAAGGCATCATCCACCGCGACCTGAAACCGCAGAACA
TCCTGCTGTCCAACCCCGCCGGCCGCCGCGCCAACCCCAACAGCATCCGC
GTCAAGATCGCTGACTTCGGCTTCGCGCGGTACCTCCAGAGCAACATGAT
GGCGGCCACACTCTGCGGCTCCCCCATGTACATGGCCCCCGAGGTCATCA
TGTCCCAGCACTACGACGGGAAGGCGGACCTGTGGAGCATCGGCACCATC
GTCTACCAGTGCCTGACGGGGAAGGCGCCCTTCCAGGCCAGCAGCCCCCA
GGACCTGCGCCTGTTCTACGAGAAGAACAAGACGTTGGTCCCCACCATCC
CCCGGGAGACCTCGGCCCCGCTGCGGCAGCTGCTCCTGGCCCTACTGCAA
CGCAACCACAAGGACCGCATGGACTTCGATGAGTTTTTTCATCACCCTTT
CCTCGATGCCAGCCCCTCGGTCAGGAAATCCCCACCCGTGCCTGTGCCCT
CGTACCCAAGCTCGGGGTCCGGCAGCAGCTCCAGCAGCAGCTCCACCTCC
CACCTGGCCTCCCCGCCGTCCCTGGGCGAGATGCAGCAGCTGCAGAAGAC
CCTGGCCTCCCCGGCTGACACCGCTGGCTTCCTGCACAGCTCCCGGGACT
CTGGTGGCAGCAAGGACTCTTCCTGTGACACAGATGACTTCGTCATGGTC
CCCGCGCAGTTTCCAGGTGACCTGGTGGCTGAGGCGCCCAGTGCCAAACC
CCCGCCAGACAGCCTGATGTGCAGTGGGAGCTCACTGGTGGCCTCTGCGG
GCTTGGAGAGCCACGGCCGGACCCCATCTCCATCCCCACCCTGCAGCAGC
TCCCCCAGTCCCTCAGGCCGGGCTGGCCCGTTCTCCAGCAGCAGGTGCGG
CGCCTCTGTCCCCATCCCAGTCCCCACGCAGGTGCAGAACTACCAGCGCA
TTGAGCGAAACCTGCAGTCACCCACCCAGTTCCAAACACCTCGGTCCTCT
GCCATCCGCAGGTCAGGCAGCACCAGCCCCCTGGGCTTTGCAAGGGCCAG
CCCCTCGCCCCCTGCCCACGCTGAGCATGGAGGCGTCCTGGCCAGGAAGA
TGTCTCTGGGTGGAGGCCGGCCCTACACGCCATCTCCTCAAGTTGGAACC
ATCCCTGAGCGGCCAGGCTGGAGCGGGACGCCCTCCCCACAGGGAGCTGA
GATGCGGGGTGGCAGGTCCCCTCGTCCAGGCTCCTCTGCACCCGAGCACT
CTCCCCGCACTTCCGGGCTGGGCTGCCGCCTGCACAGCGCCCCCAACCTG
TCTGACTTGCACGTCGTCCGCCCCAAGCTGCCCAAACCCCCCACGGACCC
CCTGGGAGCTGTGTTCAGCCCACCACAGGCCAGCCCTCCCCAGCCGTCCC
ACGGCCTGCAGTCCTGCCGGAACCTGCGGGGCTCACCCAAGCTGCCCGAC
TTCCTGCAGCGAAACCCCCTGCCCCCCATCCTGGGCTCCCCCACCAAGGC
TGTGCCCTCCTTTGACTTCCCGAAGACCCCCAGCTCCCAGAACCTGCTGG
CCCTCCTAGCCCGGCAGGGCGTGGTGATGACGCCCCCTCGAAACCGGACG
CTGCCCGACCTCTCGGAGGTGGGACCCTTCCATGGTCAGCCGTTGGGCCC
TGGCCTGCGGCCAGGCGAGGACCCCAAGGGCCCCTTTGGCCGGTCTTTCA
GCACCAGCCGCCTCACTGACCTGCTCCTTAAGGCGGCGTTTGGGACACAA
GCCCCGGACCTGGGCAGCACGGAGAGCCTGCAGGAGAAGCCCATGGAGAT
CGCACCCTCAGCTGGCTTTGGAGGGAGCCTGCACCCAGGAGCCCGTGCTG
GGGGCACCAGCAGCCCTTCCCCGGTGGTCTTCACCGTGGGCTCTCCCCCG
AGCGGGAGCACGCCCCCCCAGGGCCCCCGCACCAGGATGTTCTCAGCGGG
CCCCACTGGCTCTGCCAGCTCTTCTGCCCGCCACCTGGTGCCTGGGCCCT
GCAGCGAGGCCCCAGCCCCTGAGCTCCCTGCTCCAGGACACGGCTGCAGC
TTTGCCGACCCCATTGCTGCGAACCTGGAGGGGGCTGTGACCTTCGAGGC
CCCCGACCTCCCTGAGGAGACCCTCATGGAGCAAGAGCACACGGAGATCC
TGCGTGGCCTGCGCTTCACGCTGCTGTTCGTGCAGCACGTCCTGGAGATC
GCAGCCCTGAAGGGCAGCGCCAGTGAGGCGGCGGGGGGCCCTGAGTACCA
GCTGCAGGAGAGTGTGGTGGCCGACCAGATCAGCCTGCTGAGCCGAGAAT
GGGGCTTCGCGGAACAGCTGGTGCTGTACCTGAAGGTGGCCGAGCTACTG
TCCTCCGGCCTGCAAAGTGCCATCGACCAGATCCGGGCCGGCAAGCTCTG
CCTGTCGTCCACTGTGAAGCAGGTGGTGCGCAGGCTGAATGAGCTGTACA
AGGCCAGCGTGGTGTCCTGCCAGGGCCTGAGCCTGCGGCTGCAGCGCTTC
TTCCTGGACAAGCAGCGGCTCCTGGACCGCATTCACAGCATCACTGCCGA
GAGGCTCATCTTCAGCCACGCTGTGCAGATGGTGCAGTCGGCTGCCCTGG
ACGAGATGTTCCAGCACCGTGAGGGCTGCGTCCCACGCTACCACAAGGCC
CTGCTGCTCCTGGAGGGGCTGCAGCACATGCTCTCGGACCAGGCCGACAT
CGAGAACGTCACCAAGTGCAAGCTGTGCATTGAGCGGAGACTCTCGGCGC
TGCTGACTGGCATCTGTGCCTGAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMHHHHHHSSGLVPRGSHMA
SHHHHHHHHHHGENLYFQGMEPGRGGTETVGKFEFSRKDLIGHGAFAVVF
KGRHREKHDLEVAVKCINKKNLAKSQTLLGKEIKILKELKHENIVALYDF
QEMANSVYLVMEYCNGGDLADYLHAMRTLSEDTIRLFLQQIAGAMRLLHS
KGIIHRDLKPQNILLSNPAGRRANPNSIRVKIADFGFARYLQSNMMAATL
CGSPMYMAPEVIMSQHYDGKADLWSIGTIVYQCLTGKAPFQASSPQDLRL
FYEKNKTLVPTIPRETSAPLRQLLLALLQRNHKDRMDFDEFFHHPFLDAS
PSVRKSPPVPVPSYPSSGSGSSSSSSSTSHLASPPSLGEMQQLQKTLASP
ADTAGFLHSSRDSGGSKDSSCDTDDFVMVPAQFPGDLVAEAPSAKPPPDS
LMCSGSSLVASAGLESHGRTPSPSPPCSSSPSPSGRAGPFSSSRCGASVP
IPVPTQVQNYQRIERNLQSPTQFQTPRSSAIRRSGSTSPLGFARASPSPP
AHAEHGGVLARKMSLGGGRPYTPSPQVGTIPERPGWSGTPSPQGAEMRGG
RSPRPGSSAPEHSPRTSGLGCRLHSAPNLSDLHVVRPKLPKPPTDPLGAV
FSPPQASPPQPSHGLQSCRNLRGSPKLPDFLQRNPLPPILGSPTKAVPSF
DFPKTPSSQNLLALLARQGVVMTPPRNRTLPDLSEVGPFHGQPLGPGLRP
GEDPKGPFGRSFSTSRLTDLLLKAAFGTQAPDLGSTESLQEKPMEIAPSA
GFGGSLHPGARAGGTSSPSPVVFTVGSPPSGSTPPQGPRTRMFSAGPTGS
ASSSARHLVPGPCSEAPAPELPAPGHGCSFADPIAANLEGAVTFEAPDLP
EETLMEQEHTEILRGLRFTLLFVQHVLEIAALKGSASEAAGGPEYQLQES
VVADQISLLSREWGFAEQLVLYLKVAELLSSGLQSAIDQIRAGKLCLSST
VKQVVRRLNELYKASVVSCQGLSLRLQRFFLDKQRLLDRIHSITAERLIF
SHAVQMVQSAALDEMFQHREGCVPRYHKALLLLEGLQHMLSDQADIENVT
KCKLCIERRLSALLTGICA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU65168
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 41851. Grow at 30 degrees; silent change c1065t and coding change c2141t (P714L) (exisiting SNP)
• Low Temp: Yes