cDNA Clone TOP-2 (C. elegans)

• Plasmid: pGEX6P1 TOP-2 C.E E101-I322
• Parent Plasmid: pGEX6P1
• Expressed: GST TOP-2 E101-I322
• Genbank: NM_064135.3
• Tag: GST
• Species: Caenorhabditis elegans

Sequence of Insert

GCGGCCGCGGAGGAGAGCAAGCTGGAGCAGCGTGACATCTCATACGTGCCGGGACTCTACAAGATTTATGATGAAATTCTTGTGAATGCCGCTGACAACAAACAGAGAGATCCGAAGATGAACACAATCAAGATCACCATCAATAAGGAGAAAAATGAGATCTCTGTCTACAACAATGGCAAAGGAATCCCAGTCACTCAGCACAAAGTGGAGAAAGTCTACGTCCCAGAGCTGATTTTTGGAACTCTTCTAACATCTTCTAACTACAATGACGATGAGAAGAAAGTGACGGGAGGACGCAACGGATATGGAGCAAAGCTTTGCAACATCTTCTCCACAAAATTCACTCTGGAGACGTCTTCACGGGATTACAAAAGTGCATTCAAGCAAACGTGGATCAAAAACATGACGCGTGATGAGGAGCCGAAGATTGTTAAGAGCACCGACGAAGACTTCACGAAGATCACATTCTCGCCGGATCTGGCAAAGTTCAAGATGAAGGAGCTGGACGATGACATCTGCCATTTGATGGCTCGGCGTGCGTATGATGTGGCTGGATCCAGTAAAGGAGTCGCCGTGTTCCTCAATGGAAAACGAATTCCAATCAAAGGATTCGAGGACTACGTCCAAATGTACACTTCGCAGTTCAACAATGAAGGAGAACCCCTCAAGATCTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAEESKLEQRDISYVPGLYKIYDEILVNAADNKQRDPKMNTIKITINKEKNEISVYNNGKGIPVTQHKVEKVYVPELIFGTLLTSSNYNDDEKKVTGGRNGYGAKLCNIFSTKFTLETSSRDYKSAFKQTWIKNMTRDEEPKIVKSTDEDFTKITFSPDLAKFKMKELDDDICHLMARRAYDVAGSSKGVAVFLNGKRIPIKGFEDYVQMYTSQFNNEGEPLKI*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU68102
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Low Temp: No