cDNA Clone IRAK3

• Plasmid: pNL3.2 DUAL NF-κB-RE[NlucP/NF-κB-RE/Hygro]-CMV-FLAG-IRAK3 M1-E454
• Parent Plasmid: pNL3.2 DUAL NF-κB-RE[NlucP/NF-κB-RE/Hygro]-CMV-FLAG
• Expressed: FLAG-IRAK3 M1-E454
• Tag: FLAG
• Species: Human

Sequence of Insert

ggatccATGGCGGGGAACTGTGGGGCCCGCGGCGCGCTGTCGGCGCACAC
GCTGCTGTTCGACCTGCCGCCCGCGCTGCTCGGAGAGCTCTGCGCTGTTC
TGGACAGCTGCGACGGCGCGCTGGGTTGGCGCGGCCTGGCAGAGAGACTT
TCAAGCAGCTGGCTGGATGTTCGTCATATTGAAAAGTATGTAGACCAAGG
TAAAAGTGGAACAAGAGAATTACTTTGGTCCTGGGCACAGAAAAACAAGA
CCATCGGTGACCTTTTACAGGTCCTCCAGGAGATGGGACATCGTCGAGCT
ATTCATTTAATTACAAACTATGGAGCAGTGTTGAGTCCTTCAGAGAAGAG
TTATCAGGAAGGTGGATTTCCAAATATATTATTCAAGGAAACAGCCAATG
TCACCGTGGATAATGTTCTTATTCCTGAACATAATGAAAAAGGAGTACTG
CTTAAATCTTCCATCAGCTTTCAAAATATCATAGAAGGAACTAGAAATTT
CCACAAAGACTTCCTAATTGGAGAAGGAGAGATTTTTGAGGTATACAGAG
TGGAGATTCAAAACCTAACATATGCTGTCAAATTATTTAAACAGGAGAAA
AAAATGCAGTGTAAGAAGCATTGGAAGAGGTTTTTATCTGAGCTTGAAGT
TTTACTACTGTTTCATCACCCAAACATACTAGAGTTGGCTGCATATTTTA
CAGAGACTGAGAAGTTCTGTCTGATTTATCCATACATGAGAAATGGAACA
CTTTTTGACAGATTGCAGTGTGTAGGTGACACGGCCCCACTCCCTTGGCA
CATTCGAATCGGTATATTAATAGGAATATCCAAAGCCATTCACTACCTGC
ACAACGTTCAACCATGCTCGGTCATCTGTGGCAGTATATCAAGTGCAAAC
ATCCTTTTGGATGATCAGTTTCAACCCAAACTAACTGATTTTGCCATGGC
ACACTTCCGGTCCCACCTAGAACATCAGAGTTGTACCATAAATATGACCA
GCAGCAGCAGTAAACATCTGTGGTACATGCCAGAAGAGTACATCAGACAG
GGGAAACTTTCCATTAAAACAGATGTCTACAGCTTTGGAATTGTAATAAT
GGAAGTTCTAACAGGATGTAGAGTAGTGTTAGATGATCCAAAACATATCC
AGCTGCGGGATCTCCTTAGAGAATTGATGGAGAAGAGAGGCCTGGATTCA
TGTCTCTCATTTCTAGATAAGAAAGTGCCTCCCTGCCCTCGGAATTTCTC
TGCCAAGCTCTTCTGTTTGGCAGGCCGGTGTGCTGCAACGCGGGCAAAGT
TAAGACCATCAATGGATGAAGTTTTAAATACTCTTGAAAGTACTCAAGCC
AGCTTGTATTTTGCTGAAtaagcggccgc

Amino Acid Sequence

MDYKDDDDKGSMAGNCGARGALSAHTLLFDLPPALLGELCAVLDSCDGAL
GWRGLAERLSSSWLDVRHIEKYVDQGKSGTRELLWSWAQKNKTIGDLLQV
LQEMGHRRAIHLITNYGAVLSPSEKSYQEGGFPNILFKETANVTVDNVLI
PEHNEKGVLLKSSISFQNIIEGTRNFHKDFLIGEGEIFEVYRVEIQNLTY
AVKLFKQEKKMQCKKHWKRFLSELEVLLLFHHPNILELAAYFTETEKFCL
IYPYMRNGTLFDRLQCVGDTAPLPWHIRIGILIGISKAIHYLHNVQPCSV
ICGSISSANILLDDQFQPKLTDFAMAHFRSHLEHQSCTINMTSSSSKHLW
YMPEEYIRQGKLSIKTDVYSFGIVIMEVLTGCRVVLDDPKHIQLRDLLRE
LMEKRGLDSCLSFLDKKVPPCPRNFSAKLFCLAGRCAATRAKLRPSMDEV
LNTLESTQASLYFAE*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pNL3.2 DUAL NF-κB-RE[NlucP/NF-κB-RE/Hygro]-CMV-FLAG-IRAK3 M1-E45478.39 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU65172
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: One silent mutation at G40 (c120t) and a SNP at I147V.; PARENTAL 65115; The pNL3.2.NF-κB-RE[NlucP/NF-κB-RE/Hygro] CMV-FLAG Vector contains two expression cassettes: the first comprising five copies of an NF-κB response element (NF-κB-RE) that drives transcription of a destabilized form of NanoLuc® luciferase, an engineered small (23.3kDa) luciferase fusion protein. The NlucP reporter consists of NanoLuc® luciferase with a C-terminal fusion to PEST, a protein destabilization domain, which responds more quickly and with greater magnitude to changes in transcriptional activity than unmodified NanoLuc® luciferase. The second expression cassette expresses FLAG-IRAK3 M1-E454 from a CMV Promoter
• Low Temp: No