cDNA Clone IRAK3

• Plasmid: pNL3.2 DUAL NF-κB-RE[NlucP/NF-κB-RE/Hygro]-CMV-FLAG-IRAK3 M1-G106
• Parent Plasmid: pNL3.2 DUAL NF-κB-RE[NlucP/NF-κB-RE/Hygro]-CMV-FLAG
• Expressed: FLAG-IRAK3 M1-G106
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCATGGCGGGGAACTGTGGGGCCCGCGGCGCGCTGTCGGCGCACAC
GCTGCTGTTCGACCTGCCGCCCGCGCTGCTCGGAGAGCTCTGCGCTGTTC
TGGACAGCTGCGACGGCGCGCTGGGTTGGCGCGGCCTGGCAGAGAGACTT
TCAAGCAGCTGGCTGGATGTTCGTCATATTGAAAAGTATGTAGACCAAGG
TAAAAGTGGAACAAGAGAATTACTTTGGTCCTGGGCACAGAAAAACAAGA
CCATCGGTGACCTTTTACAGGTCCTCCAGGAGATGGGACATCGTCGAGCT
ATTCATTTAATTACAAACTATGGATAAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSMAGNCGARGALSAHTLLFDLPPALLGELCAVLDSCDGAL
GWRGLAERLSSSWLDVRHIEKYVDQGKSGTRELLWSWAQKNKTIGDLLQV
LQEMGHRRAIHLITNYG*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pNL3.2 DUAL NF-κB-RE[NlucP/NF-κB-RE/Hygro]-CMV-FLAG-IRAK3 M1-G10674.47 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU65174
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: One silent mutation at G40 (c120t) ; PARENTAL 65115; The pNL3.2.NF-κB-RE[NlucP/NF-κB-RE/Hygro] CMV-FLAG Vector contains two expression cassettes: the first comprising five copies of an NF-κB response element (NF-κB-RE) that drives transcription of a destabilized form of NanoLuc® luciferase, an engineered small (23.3kDa) luciferase fusion protein. The NlucP reporter consists of NanoLuc® luciferase with a C-terminal fusion to PEST, a protein destabilization domain, which responds more quickly and with greater magnitude to changes in transcriptional activity than unmodified NanoLuc® luciferase. The second expression cassette expresses FLAG-IRAK3 M1-G106 from a CMV Promoter
• Low Temp: No