cDNA Clone IRAK3

• Synonyms: Interleukin-1 receptor-associated kinase 3, ASRT5, IRAKM, IL-1 receptor-associated kinase M
• Plasmid: pNL3.2 DUAL NF-κB-RE[NlucP/NF-κB-RE/Hygro]-CMV-FLAG-IRAK3 W74A
• Parent Plasmid: pNL3.2
• Expressed: FLAG-IRAK3 W74A
• Genbank: NM_007199.2
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCATGGCGGGGAACTGTGGGGCCCGCGGCGCGCTGTCGGCGCACAC
GCTGCTGTTCGACCTGCCGCCCGCGCTGCTCGGAGAGCTCTGCGCTGTTC
TGGACAGCTGCGACGGCGCGCTGGGTTGGCGCGGCCTGGCAGAGAGACTT
TCAAGCAGCTGGCTGGATGTTCGTCATATTGAAAAGTATGTAGACCAAGG
TAAAAGTGGAACAAGAGAATTACTTgcGTCCTGGGCACAGAAAAACAAGA
CCATCGGTGACCTTTTACAGGTCCTCCAGGAGATGGGACATCGTCGAGCT
ATTCATTTAATTACAAACTATGGAGCAGTGTTGAGTCCTTCAGAGAAGAG
TTATCAGGAAGGTGGATTTCCAAATATATTATTCAAGGAAACAGCCAATG
TCACCGTGGATAATGTTCTTATTCCTGAACATAATGAAAAAGGAGTACTG
CTTAAATCTTCCATCAGCTTTCAAAATATCATAGAAGGAACTAGAAATTT
CCACAAAGACTTCCTAATTGGAGAAGGAGAGATTTTTGAGGTATACAGAG
TGGAGATTCAAAACCTAACATATGCTGTCAAATTATTTAAACAGGAGAAA
AAAATGCAGTGTAAGAAGCATTGGAAGAGGTTTTTATCTGAGCTTGAAGT
TTTACTACTGTTTCATCACCCAAACATACTAGAGTTGGCTGCATATTTTA
CAGAGACTGAGAAGTTCTGTCTGATTTATCCATACATGAGAAATGGAACA
CTTTTTGACAGATTGCAGTGTGTAGGTGACACGGCCCCACTCCCTTGGCA
CATTCGAATCGGTATATTAATAGGAATATCCAAAGCCATTCACTACCTGC
ACAACGTTCAACCATGCTCGGTCATCTGTGGCAGTATATCAAGTGCAAAC
ATCCTTTTGGATGATCAGTTTCAACCCAAACTAACTGATTTTGCCATGGC
ACACTTCCGGTCCCACCTAGAACATCAGAGTTGTACCATAAATATGACCA
GCAGCAGCAGTAAACATCTGTGGTACATGCCAGAAGAGTACATCAGACAG
GGGAAACTTTCCATTAAAACAGATGTCTACAGCTTTGGAATTGTAATAAT
GGAAGTTCTAACAGGATGTAGAGTAGTGTTAGATGATCCAAAACATATCC
AGCTGCGGGATCTCCTTAGAGAATTGATGGAGAAGAGAGGCCTGGATTCA
TGTCTCTCATTTCTAGATAAGAAAGTGCCTCCCTGCCCTCGGAATTTCTC
TGCCAAGCTCTTCTGTTTGGCAGGCCGGTGTGCTGCAACGCGGGCAAAGT
TAAGACCATCAATGGATGAAGTTTTAAATACTCTTGAAAGTACTCAAGCC
AGCTTGTATTTTGCTGAAGATCCTCCCACATCACTAAAGTCCTTCAGGTG
TCCTTCTCCTCTATTCCTGGAGAATGTACCAAGTATTCCAGTGGAAGATG
ATGAAAGCCAGAATAACAATTTACTACCTTCTGATGAAGGCCTGAGGATA
GACAGAATGACTCAGAAAACTCCTTTTGAATGCAGCCAGTCTGAGGTTAT
GTTTCTGAGCTTGGACAAAAAGCCAGAGAGCAAGAGAAATGAGGAAGCTT
GCAACATGCCCAGTTCTTCTTGTGAAGAAAGTTGGTTCCCAAAGTATATA
GTTCCATCCCAGGACTTAAGGCCCTATAAGGTAAATATAGATCCTTCTTC
AGAAGCTCCAGGGCATTCTTGCAGGAGCAGGCCAGTGGAGAGCAGCTGTT
CCTCCAAATTTTCCTGGGATGAATATGAACAGTACAAAAAAGAATAAGCG
GCCGC

Amino Acid Sequence

MDYKDDDDKGSMAGNCGARGALSAHTLLFDLPPALLGELCAVLDSCDGAL
GWRGLAERLSSSWLDVRHIEKYVDQGKSGTRELLASWAQKNKTIGDLLQV
LQEMGHRRAIHLITNYGAVLSPSEKSYQEGGFPNILFKETANVTVDNVLI
PEHNEKGVLLKSSISFQNIIEGTRNFHKDFLIGEGEIFEVYRVEIQNLTY
AVKLFKQEKKMQCKKHWKRFLSELEVLLLFHHPNILELAAYFTETEKFCL
IYPYMRNGTLFDRLQCVGDTAPLPWHIRIGILIGISKAIHYLHNVQPCSV
ICGSISSANILLDDQFQPKLTDFAMAHFRSHLEHQSCTINMTSSSSKHLW
YMPEEYIRQGKLSIKTDVYSFGIVIMEVLTGCRVVLDDPKHIQLRDLLRE
LMEKRGLDSCLSFLDKKVPPCPRNFSAKLFCLAGRCAATRAKLRPSMDEV
LNTLESTQASLYFAEDPPTSLKSFRCPSPLFLENVPSIPVEDDESQNNNL
LPSDEGLRIDRMTQKTPFECSQSEVMFLSLDKKPESKRNEEACNMPSSSC
EESWFPKYIVPSQDLRPYKVNIDPSSEAPGHSCRSRPVESSCSSKFSWDE
YEQYKKE*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pNL3.2 DUAL NF-κB-RE[NlucP/NF-κB-RE/Hygro]-CMV-FLAG-IRAK3 W74A 182.65 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU65184
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: One silent mutation at Gly40 (c120t) and a SNP at I147V.; Parental 65115 Dual NanoLuc reporter; The co-expressed NlucP reporter consists of NanoLuc® luciferase with a C-terminal fusion to PEST (see 65115 Vector data sheet for more info); 65157 WT template snap gene map attached
• Low Temp: No