cDNA Clone EDE1/YBL47C

• Synonyms: YBL47C
• Plasmid: p416 GPD 1000-EDE1/YBL47C-400
• Parent Plasmid: p416 GPD (LMB)
• Expressed: EDE1
• Genbank: ID: 852233
• Species: Yeast

Sequence of Insert

ggatccAATCCTTCCTCTTCATCCTCGTCATCGTAGCCTAGGTTGACACTCATTATAGAGTCGATTTCTCTAATAAAGGGTGCCAACTCCCTAGTATTGTTTTCGGTCATCCACGGTATCTTCATTAGTGAGACATCTTCTTGATTCTTGATGAAGTTAATATCTTTCTCCTTATCGTCCTCTTGCTTCTCACCGTGTGTTTTATCAAAAACCTGCCAACTGCTAGTTACCATGCAACATTTTTCTAGCGCATTTACAAACTTCTCCAATTCATTGATCTTGTAATATTTGAAAGGATCATAACACAATTCGCATATCCGAAGTATTGTGAATGGAAATGTATGATTTTGTAAAAAATTATCATTCAGAAACTTACCCAGCCAATTTAATCTAGTAACGAGATTTCTCGCATCGTTCATTTCATTCTTAAGGTTTATAAATAGGTCAAACGGTATTGTTTCAGTGATATGCCGGATTAAATCAGGCAAAACTTGCTCGGGACCCGCCGTCACCAATCGCGTCTTGTCGCCCTGTTTCACCACATCGCTAAGTAATTCATAAAGGCTGATAGACTTGATACCCATCATATTATTATCCACATCATCCAACATCGTCGAGCTCATAGCTTTTTATTGTGCTTTGACAATCGATGAGAACACCAACAAAAAATGATAATAGGTATTGTTAGCTGTGTCAAAATACATGCTTTTGATCGCGAAATGAAAAACTACACAATCATTACCCGTCGGCGCTTCGGAGGGGGATTAGATTTCTCCTATAATCAAAATCTTCAACTATTACAAACCCCTTTTCAGAAGCACATTGAAAACAACACACAACAGAGCACTTTGTGATAGGCAATAGAGTGCTTTTAAATAGAGAATTACTGGTTCTCGCGAAATAATATAGCGCGTTGAAACTAATAAATAGATTCGTGAAGCTCTAGATCTATCTTTTTTCCTGTTTTTATCTGTGTGTTTTCAATAGGATTCGAACGATATAGGCCATGGCATCTATTACTTTTAGAACTCCATTATCCTCTCAGGAACAAGCCTTTTATAATCAGAAATTTCACCAATTGGATACGGAAGATCTCGGGGTGGTCACTGGCGAGGCAGTCAGACCGCTCTTCGCGTCTTCTGGTCTTCCAGGGCAATTACTATCGCAAGTGTGGGCTACCGTCGATATAGACAATAAAGGGTTTTTGAACTTGAACGAGTTTTCGGCTGCTTTGAGAATGATCGCTCAATTGCAAAATGCTCCCAACCAACCAATTTCCGCTGCCCTATATGAGAGCACACCTACTCAACTGGCTTCTTTTTCTATAAATCAGAACCCAGCCCCTATGCAAAGCGGTAGTGCAACCGGCAATACAAACAACACTGATATTCCTGCCTTATCCTCAAATGATATTGCTAAATTTTCACAACTGTTTGATAGGACAGCAAAGGGTGCTCAAACTGTTGCTGGTGATAAAGCCAAGGATATATTTTTAAAGGCAAGATTACCAAATCAAACTTTAGGTGAAATTTGGGCTCTTTGTGATAGAGACGCATCCGGTGTCTTAGACAAATCTGAGTTTATAATGGCCATGTATTTGATCCAACTATGTATGTCTCATCATCCTAGTATGAACACACCACCGGCCGTGTTGCCTACTCAGCTGTGGGATTCTATTCGTTTAGAACCTGTTGTTGTTAATCAACCAAACAGGACTACTCCTTTGAGTGCTAACTCCACTGGCGTATCTTCACTAACAAGACACTCTACTATATCACGTCTATCTACTGGTGCATTCAGTAATGCTGCTTCAGATTGGTCTTTAAGTTTTGAAAAAAAACAACAGTTTGATGCAATTTTTGATTCATTAGATAAGCAACATGCTGGTTCGCTAAGCTCTGCTGTGTTGGTTCCATTCTTCTTATCCTCTAGATTAAACCAAGAGACGTTAGCCACCATCTGGGACTTAGCTGACATCCACAACAATGCTGAATTCACCAAATTGGAATTTGCTATTGCCATGTTCTTAATTCAAAAAAAGAATGCTGGTGTCGAATTACCGGATGTTATCCCCAATGAATTATTACAGTCCCCCGCTTTAGGACTTTATCCGCCAAACCCACTTCCTCAACAACAAAGTGCACCTCAAATTGCCATCCCCTCAAGAGCAAGTAAGCCTTCTTTACAGGATATGCCACACCAAGTTTCTGCCCCAGCAGTTAATACTCAACCAACAGTTCCACAAGTTCTTCCTCAAAACTCAAATAACGGTTCTTTGAATGATTTACTGGCTTTGAACCCTTCATTCAGCTCTCCTTCTCCCACTAAAGCACAAACTGTGGTTCAAAACAACACAAATAATAGTTTTTCATATGATAATAACAATGGCCAAGCAACATTACAACAACAACAGCCACAGCAGCCTCCACCTTTAACTCACTCCTCCTCTGGATTGAAGAAATTTACTCCCACCTCAAACTTCGGTCAAAGTATAATCAAGGAAGAACCAGAGGAACAAGAGCAACTAAGGGAATCCAGTGATACTTTTAGCGCTCAGCCTCCGCCAGTTCCAAAACATGCTTCAAGTCCAGTCAAACGTACAGCTTCAACAACACTGCCACAAGTTCCCAACTTTTCTGTGTTTAGCATGCCAGCAGGCGCTGCCACTAGCGCTGCCACTGGTGCTGCTGTAGGCGCCGCAGTGGGTGCTGCTGCTTTAGGCGCATCCGCTTTTTCAAGATCTTCCAATAATGCATTCAAGAACCAGGATTTATTTGCAGATGGTGAAGCTTCTGCTCAGTTATCTAATGCTACCACAGAAATGGCTAATTTATCTAACCAAGTAAACTCCCTGTCCAAGCAGGCAAGTATAACTAATGACAAAAAGTCAAGAGCGACCCAGGAGTTGAAACGTGTTACTGAGATGAAAAATTCCATTCAAATCAAATTGAACAATTTACGTTCTACCCATGACCAAAATGTTAAACAAACGGAACAACTGGAAGCACAAGTACTTCAAGTAAACAAAGAAAACGAAACATTAGCGCAACAGTTGGCTGTATCCGAAGCAAACTACCATGCCGCTGAGTCCAAACTCAATGAACTAACTACTGATTTGCAAGAATCTCAAACAAAGAACGCAGAATTAAAAGAACAAATTACGAATTTGAACTCGATGACAGCCTCCTTACAGTCACAATTAAATGAAAAACAACAACAGGTCAAGCAAGAAAGGTCAATGGTAGATGTTAATTCAAAACAGCTAGAGTTGAATCAAGTCACAGTAGCAAACTTGCAGAAGGAAATCGATGGTCTTGGCGAAAAGATAAGCGTTTACTTAACTAAACAAAAGGAGTTAAATGACTACCAAAAGACCGTGGAGGAGCAACACGCACAATTACAAGCTAAATACCAGGATCTGTCCAACAAGGATACTGATTTAACGGACCGTGAAAAACAATTGGAAGAACGTAACAGACAAATTGAAGAACAAGAAAATCTCTACCATCAGCACGTTTCTAAATTGCAAGAAATGTTCGACGATCTATCACAGAGAAAAGCTTCTTTTGAAAAAGCTGATCAAGAGTTAAAGGAGAGAAACATCGAATACGCTAACAATGTGAGAGAGTTATCCGAAAGACAAATGAATTTAGCTATGGGACAATTACCTGAGGATGCTAAGGACATTATTGCGAAAAGCGCCTCTAATACAGATACAACAACCAAAGAAGCCACATCAAGAGGAAATGTTCACGAAGACACTGTATCCAAATTTGTCGAAACTACTGTTGAGAATTCAAACTTGAATGTTAATCGGGTTAAAGACGATGAAGAAAAGACAGAAAGAACCGAAAGTGACGTATTTGATAGAGACGTTCCAACTTTGGGATCTCAAAGCGATAGTGAAAATGCCAATACAAACAACGGCACCCAGTCAGGAAATGAGACAGCAAATCCAAACTTAACTGAAACATTAAGTGATAGGTTTGATGGTGATTTGAATGAATATGGTATTCCAAGAAGTCAATCCTTGACATCGTCTGTTGCAAACAATGCTCCTCAATCCGTAAGAGATGATGTTGAGCTACCGGAAACGTTGGAAGAACGTGACACCATCAATAATACTGCGAATAGAGATAATACTGGAAACTTATCTCACATTCCTGGTGAATGGGAAGCTACCCCAGCTACCGCTTCTACTGATGTTTTGAGCAACGAAACTACAGAAGTTATTGAAGACGGAAGTACCACAAAAAGGGCTAATTCAAATGAAGACGGTGAGTCGGTCAGTTCTATCCAAGAATCTCCAAAAATCTCTGCTCAACCAAAAGCAAAGACAATAAATGAAGAGTTTCCACCAATTCAGGAATTGCATATTGACGAAAGCGATTCATCCTCTTCAGACGATGATGAGTTTGAAGATACCAGAGAAATTCCATCAGCAACAGTGAAGACCCTTCAAACTCCCTATAATGCCCAGCCCACATCAAGTTTGGAAATCCACACGGAACAAGTCATAAAATATCCCGCTCCAGGCACTTCTCCTTCACATAATGAAGGTAACTCAAAAAAGGCAAGCACCAATTCCATTTTGCCTGTTAAGGACGAATTTGATGATGAATTCGCAGGGTTAGAACAAGCGGCTGTGGAGGAAGATAATGGTGCTGATTCGGAATCTGAATTTGAGAATGTTGCGAATGCTGGGTCGATGGAGCAATTTGAAACCATAGACCATAAGGATCTCGATGACGAACTACAAATGAACGCTTTTACAGGGACGTTAACTAGTTCCTCTAACCCAACTATCCCAAAACCACAAGTACAACAACAGTCAACCAGTGACCCTGCTCAAGTAAGCAACGATGAGTGGGACGAGATTTTTGCTGGATTTGGTAACTCCAAAGCTGAACCAACCAAAGTAGCTACCCCAAGCATTCCACAACAACCAATTCCATTGAAGAATGATCCTATCGTTGATGCTAGCTTGTCAAAAGGTCCAATAGTTAACCGCGGGGTTGCAACTACTCCAAAGTCTCTAGCCGTCGAGGAGTTAAGTGGCATGGGATTTACTGAAGAAGAAGCGCACAATGCCTTGGAAAAGTGCAACTGGGATCTAGAAGCCGCCACTAACTTTTTGTTGGATAGTGCTTAGTCTGTAATGGACCATTTCGTCTTCTTTTTGTACTTCTTCCTCGAAGCTGAATCATATTTACCCTTTTCCAGGATCGTCCTTGTATATTGTGAATTATTCTAAAAGTAGAATATGTTGTACTTTAAAATTGTTATAGTTATCATCATCTATTTTTCCATTTTATAAAATTTTTACGTTAGACAGTTATGTATTGAAACTGCTTCATAAGTTTTGAAAATCATCCCAGTTTGGTAAATACACTAACCAAAAAAAAAAAAAGCTCTACCACATAATCTTTGGGCGTATTACAACATGGGAAACACGACATTATTGAAAATACAAAAAAAGTAAAGTAAGACTCGTTCAGGGCATAAGTCAATGGCCCTGTAAGAAAAAACAAAGTAGCTAGGCTCATAAGAGAcccggg

Amino Acid Sequence

MASITFRTPLSSQEQAFYNQKFHQLDTEDLGVVTGEAVRPLFASSGLPGQLLSQVWATVDIDNKGFLNLNEFSAALRMIAQLQNAPNQPISAALYESTPTQLASFSINQNPAPMQSGSATGNTNNTDIPALSSNDIAKFSQLFDRTAKGAQTVAGDKAKDIFLKARLPNQTLGEIWALCDRDASGVLDKSEFIMAMYLIQLCMSHHPSMNTPPAVLPTQLWDSIRLEPVVVNQPNRTTPLSANSTGVSSLTRHSTISRLSTGAFSNAASDWSLSFEKKQQFDAIFDSLDKQHAGSLSSAVLVPFFLSSRLNQETLATIWDLADIHNNAEFTKLEFAIAMFLIQKKNAGVELPDVIPNELLQSPALGLYPPNPLPQQQSAPQIAIPSRASKPSLQDMPHQVSAPAVNTQPTVPQVLPQNSNNGSLNDLLALNPSFSSPSPTKAQTVVQNNTNNSFSYDNNNGQATLQQQQPQQPPPLTHSSSGLKKFTPTSNFGQSIIKEEPEEQEQLRESSDTFSAQPPPVPKHASSPVKRTASTTLPQVPNFSVFSMPAGAATSAATGAAVGAAVGAAALGASAFSRSSNNAFKNQDLFADGEASAQLSNATTEMANLSNQVNSLSKQASITNDKKSRATQELKRVTEMKNSIQIKLNNLRSTHDQNVKQTEQLEAQVLQVNKENETLAQQLAVSEANYHAAESKLNELTTDLQESQTKNAELKEQITNLNSMTASLQSQLNEKQQQVKQERSMVDVNSKQLELNQVTVANLQKEIDGLGEKISVYLTKQKELNDYQKTVEEQHAQLQAKYQDLSNKDTDLTDREKQLEERNRQIEEQENLYHQHVSKLQEMFDDLSQRKASFEKADQELKERNIEYANNVRELSERQMNLAMGQLPEDAKDIIAKSASNTDTTTKEATSRGNVHEDTVSKFVETTVENSNLNVNRVKDDEEKTERTESDVFDRDVPTLGSQSDSENANTNNGTQSGNETANPNLTETLSDRFDGDLNEYGIPRSQSLTSSVANNAPQSVRDDVELPETLEERDTINNTANRDNTGNLSHIPGEWEATPATASTDVLSNETTEVIEDGSTTKRANSNEDGESVSSIQESPKISAQPKAKTINEEFPPIQELHIDESDSSSSDDDEFEDTREIPSATVKTLQTPYNAQPTSSLEIHTEQVIKYPAPGTSPSHNEGNSKKASTNSILPVKDEFDDEFAGLEQAAVEEDNGADSESEFENVANAGSMEQFETIDHKDLDDELQMNAFTGTLTSSSNPTIPKPQVQQQSTSDPAQVSNDEWDEIFAGFGNSKAEPTKVATPSIPQQPIPLKNDPIVDASLSKGPIVNRGVATTPKSLAVEELSGMGFTEEEAHNALEKCNWDLEAATNFLLDSA*

• Vector Type: Yeast
• Antibiotic: Amp
• DU Number: DU66143
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Cloning of (YBL047C) EDE1 (+ 1000 bp upstream and 400 bp downstream of EDE1 ORF) in the vector p416-GPD (66025)
• Low Temp: No