cDNA Clone Laforin

• Synonyms: EPM2, MELF, EPM2A
• Plasmid: pGEX6P-1-Laforin C266S
• Parent Plasmid: pGEX6P-1
• Expressed: GST-Laforin[C266S]
• Genbank: NM_005670
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCCGCTTCCGCTTTGGGGTGGTGGTGCCACCCGCCGTGGCCGGCGC
CCGGCCGGAGCTGCTGGTGGTGGGGTCGCGGCCCGAGCTGGGGCGTTGGG
AGCCGCGCGGTGCCGTCCGCCTGAGGCCGGCCGGCACCGCGGCGGGCGAC
GGGGCCCTGGCGCTGCAGGAGCCGGGCCTGTGGCTCGGGGAGGTGGAGCT
GGCGGCCGAGGAGGCGGCGCAGGACGGGGCGGAGCCGGGCCGCGTGGACA
CGTTCTGGTACAAGTTCCTGAAGCGGGAGCCGGGAGGAGAGCTCTCCTGG
GAAGGCAATGGACCTCATCATGACCGTTGCTGTACTTACAATGAAAACAA
CTTGGTGGATGGTGTGTATTGTCTCCCAATAGGACACTGGATTGAGGCCA
CTGGACACACCAATGAAATGAAGCACACAACAGACTTCTATTTTAATATT
GCAGGCCACCAAGCCATGCATTATTCAAGAATTCTACCAAATATCTGGCT
GGGTAGCTGCCCTCGTCAGGTGGAACATGTAACCATCAAACTGAAGCATG
AATTGGGGATTACAGCTGTAATGAATTTCCAGACTGAATGGGATATTGTA
CAGAATTCCTCAGGCTGTAACCGCTACCCAGAGCCCATGACTCCAGACAC
TATGATTAAACTATATAGGGAAGAAGGCTTGGCCTACATCTGGATGCCAA
CACCAGATATGAGCACCGAAGGCCGAGTACAGATGCTGCCCCAGGCGGTG
TGCCTGCTGCATGCGCTGCTGGAGAAGGGACACATCGTGTACGTGCACTc
CAACGCTGGGGTGGGCCGCTCCACAGCGGCTGTCTGCGGCTGGCTCCAGT
ATGTGATGGGCTGGAATCTGAGGAAGGTGCAGTATTTCCTCATGGCCAAG
AGGCCGGCTGTCTACATTGACGAAGAGGCCTTGGCCCGGGCACAAGAAGA
TTTTTTCCAGAAATTTGGGAAGGTTCGTTCTTCTGTGTGTAGCCTGTAGG
GATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSRFRFGVVVPPAVAGARPEL
LVVGSRPELGRWEPRGAVRLRPAGTAAGDGALALQEPGLWLGEVELAAEE
AAQDGAEPGRVDTFWYKFLKREPGGELSWEGNGPHHDRCCTYNENNLVDG
VYCLPIGHWIEATGHTNEMKHTTDFYFNIAGHQAMHYSRILPNIWLGSCP
RQVEHVTIKLKHELGITAVMNFQTEWDIVQNSSGCNRYPEPMTPDTMIKL
YREEGLAYIWMPTPDMSTEGRVQMLPQAVCLLHALLEKGHIVYVHSNAGV
GRSTAAVCGWLQYVMGWNLRKVQYFLMAKRPAVYIDEEALARAQEDFFQK
FGKVRSSVCSL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU65323
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Silent change at Thr274 (ACC > ACA) compared to NCBI ref.; ORF has no native initiating methionine
• Low Temp: No