cDNA Clone PHLPP2

• Synonyms: PH domain and leucine rich repeat protein phosphatase 2, PPM3B, PHLPPL
• Plasmid: HA-PHLPP2
• Parent Plasmid: HA-PHLPP2
• Expressed: HA-PHLPP2
• Genbank: Gene 23035 NM_015020.3 → NP_055835.2
• Tag: HA
• Species: Human

Sequence of Insert

gctagcATGAAACGCAATGGGAGCAGAAATTGTTTGAATAGGAGAAGTAG
GTTTGGTTCTCGAGAAAGAGACTGGCTAAGAGAAGATGTAAAGAGAGGCT
GTGTTTACCTTTATGGAGCAGACACTACCACTGCCACTACAACCACCACC
ACCTCCTCTTCCTCTTCCTCCTCCTCCTCTTCCTCTGACTTACATCTCGT
CCTTTGCACTGTAGAGACACCAGCATCAGAAATATGTGCTGGAGAGGGAA
GAGAAAGTCTTTATTTACAGCTTCATGGAGACCTGGTCAGGAGACTGGAA
CCTACTGAACGACCTCTTCAGATCGTTTATGATTACTTATCCAGGCTGGG
ATTTGATGATCCTGTGCGCATACAGGAGGAGGCTACAAATCCTGACCTCG
GCTGTATGATTCGATTTTATGGTGAAAAACCATGCCACATGGATCGTTTG
GATCGAATCCTATTGTCTGGCATCTATAATGTACGCAAGGGAAAGACCCA
GCTGCATAAGTGGGCTGAGCGCCTAGTTGTCCTCTGTGGTACCTGCCTTA
TCGTTTCCTCAGTGAAGGATTGTCAAACTGGAAAGATGCACATTTTGCCT
CTGGTTGGTGGAAAGATAGAAGAAGTGAAGCGACGGCAATACTCCCTTGC
TTTCAGCTCAGCAGGAGCCCAAGCTCAGACCTATCATGTCAGCTTCGAGA
CTTTGGCCGAGTACCAGCGATGGCAACGGCAAGCATCCAAGGTGGTGTCC
CAGCGAATCAGTACCGTGGATCTCTCGTGTTACAGCCTCGAGGAGGTTCC
TGAGCATCTCTTCTATAGTCAAGATATTACCTACCTCAACTTGCGACACA
ACTTCATGCAGTTAGAAAGACCCGGAGGCCTCGATACACTCTACAAATTT
TCTCAACTGAAGGGCCTGAACTTGTCCCATAATAAACTTGGGTTGTTTCC
TATATTGTTATGCGAGATCTCTACCCTGACTGAGCTCAACCTTTCCTGTA
ATGGATTTCATGACCTACCAAGTCAAATTGGCAATCTGCTAAATCTTCAA
ACCCTCTGTCTTGATGGCAACTTTCTGACTACTTTACCTGAAGAATTGGG
AAATCTACAACAGCTTTCCTCCTTGGGAATTTCCTTCAACAACTTTAGTC
AAATTCCTGAGGTTTATGAGAAACTCACTATGTTAGATAGAGTGGTTATG
GCAGGAAATTGCCTGGAAGTCCTGAACTTAGGGGTGCTGAATAGGATGAA
CCATATCAAGCATGTGGATTTAAGGATGAACCATTTGAAAACCATGGTTA
TTGAAAATCTGGAGGGAAATAAACACATCACCCACGTGGATCTGCGGGAC
AACCGACTGACTGACTTGGATCTTAGCTCCTTATGCAGCTTGGAACAGCT
GCACTGTGGGCGGAATCAGCTGAGGGAGCTAACACTCAGTGGCTTTTCCC
TTCGGACCCTCTATGCCAGTTCCAACAGGCTGACAGCAGTGAACGTCTAT
CCAGTACCCAGCCTGCTCACTTTCTTGGATCTCTCCCGAAACCTGCTAGA
GTGTGTCCCTGACTGGGCCTGTGAAGCAAAGAAGATAGAAGTATTAGATG
TGAGCTATAATCTTCTCACAGAGGTTCCCGTGAGAATTCTGAGTAGCTTG
AGTCTTAGAAAACTGATGCTGGGACACAATCATGTGCAAAACCTTCCAAC
ACTGGTAGAGCACATCCCCCTCGAGGTGCTGGATCTTCAGCATAATGCAC
TCACGAGGCTGCCAGACACCCTCTTCTCCAAGGCCTTAAATCTCAGATAC
TTGAATGCATCTGCAAATAGTCTGGAGTCTTTACCATCCGCCTGCACTGG
AGAGGAGAGTTTGAGTATGCTGCAGCTGCTTTATCTGACCAACAATCTCC
TGACGGATCAGTGCATACCTGTCCTGGTAGGGCACCTGCACCTGCGAATC
TTGCACCTTGCAAACAATCAGTTACAGACCTTTCCTGCAAGCAAACTAAA
TAAATTGGAGCAATTGGAGGAACTGAACCTAAGTGGCAACAAGCTTAAAA
CCATTCCCACAACCATAGCAAACTGTAAAAGGCTGCACACCCTTGTTGCA
CACTCCAACAACATCAGCATTTTCCCAGAAATACTGCAGTTGCCTCAGAT
CCAGTTTGTAGACCTAAGTTGCAACGACTTGACAGAAATCCTGATTCCAG
AGGCTTTGCCTGCTACATTACAAGACCTTGACCTGACTGGAAATACAAAT
CTGGTTCTGGAACACAAGACACTGGACATATTTAGCCATATCACAACCCT
GAAAATTGATCAGAAACCTTTGCCAACCACAGATTCTACAGTTACGTCAA
CCTTCTGGAGCCATGGACTGGCTGAGATGGCAGGGCAGAGAAATAAGCTG
TGTGTCTCAGCACTTGCTATGGATAGCTTTGCAGAGGGGGTGGGAGCTGT
GTATGGCATGTTTGATGGAGACCGAAATGAGGAGCTCCCGCGCCTGCTGC
AGTGTACGATGGCAGATGTGCTTTTAGAAGAGGTACAGCAGTCAACTAAT
GACACAGTTTTCATGGCTAACACCTTCTTGGTATCTCACAGGAAATTAGG
AATGGCTGGCCAGAAGTTGGGCTCCTCCGCTCTCCTGTGCTACATCCGCC
CTGACACTGCCGATCCAGCAAGTAGCTTTAGCTTGACTGTAGCCAATGTT
GGCACGTGCCAAGCAGTCCTGTGCCGAGGTGGGAAGCCAGTGCCCCTCTC
TAAAGTCTTCAGCCTGGAGCAGGACCCAGAGGAGGCTCAAAGGGTGAAGG
ACCAAAAAGCCATCATCACAGAGGACAACAAAGTGAATGGGGTAACCTGC
TGTACCCGGATGCTGGGCTGTACATACCTCTACCCTTGGATCCTCCCCAA
GCCCCACATATCTTCCACTCCGCTGACCATTCAAGATGAGTTGCTGATTC
TGGGAAACAAAGCATTGTGGGAACACTTGTCCTACACAGAAGCTGTCAAT
GCTGTACGTCACGTACAAGACCCATTAGCAGCTGCTAAGAAGCTGTGCAC
ATTAGCGCAGAGCTATGGCTGTCAGGACAATGTAGGGGCGATGGTAGTTT
ATTTGAATATTGGTGAGGAAGGCTGCACTTGTGAAATGAATGGGCTCACC
CTCCCAGGTCCTGTGGGATTTGCTTCAACCACCACTATCAAGGATGCCCC
TAAGCCAGCCACTCCATCCTCTAGCAGTGGGATTGCCTCTGAGTTCAGCA
GTGAGATGTCCACCTCAGAGGTGAGCAGTGAAGTGGGGTCCACTGCTTCT
GATGAGCATAATGCTGGGGGCCTGGACACTGCCTTGCTTCCGAGGCCAGA
GCGGCGCTGCAGCCTCCACCCAACACCCACCTCTGGGCTGTTTCAGCGCC
AGCCTTCTTCTGCTACCTTCTCCAGTAACCAGTCTGACAACGGCCTGGAC
AGTGATGATGACCAGCCCGTTGAGGGGGTCATAACCAATGGCAGCAAGGT
AGAGGTGGAAGTAGACATCCACTGCTGCAGGGGGAGGGATCTGGAGAACT
CACCCCCTCTCATAGAGAGTTCTCCTACCCTGTGTTCTGAGGAACATGCT
AGAGGGTCGTGTTTTGGGATCCGAAGACAGAACAGTGTGAATAGTGGCAT
GCTCCTGCCAATGAGCAAGGACAGGATGGAGTTACAGAAGTCTCCCTCCA
CCTCCTGCCTCTATGGGAAGAAACTCTCCAATGGCTCTATTGTGCCCCTA
GAGGACAGCCTGAACCTCATTGAAGTGGCCACAGAAGTGCCCAAGAGGAA
AACTGGCTATTTTGCTGCCCCCACTCAGATGGAACCAGAGGACCAGTTTG
TTGTGCCTCATGACCTGGAAGAAGAAGTGAAGGAACAAATGAAACAGCAC
CAGGACAGCCGGCTCGAGCCTGAGCCCCATGAAGAGGATCGGACCGAGCC
CCCGGAGGAGTTCGACACAGCACTATGAgcggccgc

Amino Acid Sequence

MYPYDVPDYAGSFEEFASMKRNGSRNCLNRRSRFGSRERDWLREDVKRGC
VYLYGADTTTATTTTTTSSSSSSSSSSSDLHLVLCTVETPASEICAGEGR
ESLYLQLHGDLVRRLEPTERPLQIVYDYLSRLGFDDPVRIQEEATNPDLG
CMIRFYGEKPCHMDRLDRILLSGIYNVRKGKTQLHKWAERLVVLCGTCLI
VSSVKDCQTGKMHILPLVGGKIEEVKRRQYSLAFSSAGAQAQTYHVSFET
LAEYQRWQRQASKVVSQRISTVDLSCYSLEEVPEHLFYSQDITYLNLRHN
FMQLERPGGLDTLYKFSQLKGLNLSHNKLGLFPILLCEISTLTELNLSCN
GFHDLPSQIGNLLNLQTLCLDGNFLTTLPEELGNLQQLSSLGISFNNFSQ
IPEVYEKLTMLDRVVMAGNCLEVLNLGVLNRMNHIKHVDLRMNHLKTMVI
ENLEGNKHITHVDLRDNRLTDLDLSSLCSLEQLHCGRNQLRELTLSGFSL
RTLYASSNRLTAVNVYPVPSLLTFLDLSRNLLECVPDWACEAKKIEVLDV
SYNLLTEVPVRILSSLSLRKLMLGHNHVQNLPTLVEHIPLEVLDLQHNAL
TRLPDTLFSKALNLRYLNASANSLESLPSACTGEESLSMLQLLYLTNNLL
TDQCIPVLVGHLHLRILHLANNQLQTFPASKLNKLEQLEELNLSGNKLKT
IPTTIANCKRLHTLVAHSNNISIFPEILQLPQIQFVDLSCNDLTEILIPE
ALPATLQDLDLTGNTNLVLEHKTLDIFSHITTLKIDQKPLPTTDSTVTST
FWSHGLAEMAGQRNKLCVSALAMDSFAEGVGAVYGMFDGDRNEELPRLLQ
CTMADVLLEEVQQSTNDTVFMANTFLVSHRKLGMAGQKLGSSALLCYIRP
DTADPASSFSLTVANVGTCQAVLCRGGKPVPLSKVFSLEQDPEEAQRVKD
QKAIITEDNKVNGVTCCTRMLGCTYLYPWILPKPHISSTPLTIQDELLIL
GNKALWEHLSYTEAVNAVRHVQDPLAAAKKLCTLAQSYGCQDNVGAMVVY
LNIGEEGCTCEMNGLTLPGPVGFASTTTIKDAPKPATPSSSSGIASEFSS
EMSTSEVSSEVGSTASDEHNAGGLDTALLPRPERRCSLHPTPTSGLFQRQ
PSSATFSSNQSDNGLDSDDDQPVEGVITNGSKVEVEVDIHCCRGRDLENS
PPLIESSPTLCSEEHARGSCFGIRRQNSVNSGMLLPMSKDRMELQKSPST
SCLYGKKLSNGSIVPLEDSLNLIEVATEVPKRKTGYFAAPTQMEPEDQFV
VPHDLEEEVKEQMKQHQDSRLEPEPHEEDRTEPPEEFDTAL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU65272
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: isoform 1; Parental 44059
• Low Temp: No