cDNA Clone LRRK1

• Plasmid: pcDNA5-FRT/TO-GFP-LRRK1 K746G D1409A
• Parent Plasmid: pcDNA5-FRT/TO-GFP
• Expressed: GFP-LRRK1 K746G D1409A
• Genbank: NM_024652.4
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGGCTGGCATGTCGCAAAGACCCCCCAGCATGTACTGGTGTGT
GGGGCCGGAGGAGTCAGCTGTGTGTCCAGAACGTGCCATGGAGACGCTTA
ACGGTGCCGGGGACACGGGCGGCAAGCCGTCCACGCGGGGCGGTGACCCT
GCAGCGCGGTCCCGCAGGACGGAAGGCATCCGCGCCGCGTACAGGCGGGG
AGACCGCGGCGGCGCCCGGGACCTGCTGGAGGAGGCCTGCGACCAGTGCG
CGTCCCAGCTGGAAAAGGGCCAGCTTCTGAGCATCCCGGCAGCCTATGGG
GATCTGGAGATGGTCCGCTACCTACTCAGCAAGAGACTGGTGGAGCTGCC
CACCGAGCCCACGGATGACAACCCAGCCGTGGTGGCAGCGTATTTTGGAC
ACACGGCAGTTGTGCAGGAATTGCTTGAGTCCTTACCAGGTCCCTGCAGT
CCCCAGCGGCTTCTGAACTGGATGCTGGCCTTGGCTTGCCAGCGAGGGCA
CCTGGGGGTTGTGAAGCTCCTGGTCCTGACGCACGGGGCTGACCCGGAGA
GCTACGCTGTCAGGAAGAATGAGTTCCCTGTCATCGTGCGCTTGCCCCTG
TATGCGGCCATCAAGTCAGGGAATGAAGACATTGCAATATTCCTGCTTCG
GCATGGGGCCTATTTCTGTTCCTACATCTTGCTGGATAGTCCTGACCCCA
GCAAACATCTGCTGAGAAAGTACTTCATTGAAGCCAGTCCCTTGCCCAGC
AGTTATCCGGGAAAAACAGCTCTCCGTGTGAAATGGTCCCATCTCAGACT
GCCCTGGGTAGACCTAGACTGGCTCATAGACATCTCCTGCCAGATCACGG
AGCTCGACCTTTCTGCCAACTGCCTGGCGACCCTCCCCTCGGTTATCCCC
TGGGGCCTCATCAATCTCCGGAAGCTGAACCTCTCCGACAACCACCTGGG
GGAGCTGCCTGGCGTGCAGTCATCGGACGAAATCATCTGTTCCAGGCTAC
TTGAAATTGACATTTCCAGCAACAAGTTGTCCCACCTCCCTCCTGGATTC
TTGCACCTCTCAAAACTTCAAAAACTGACAGCTTCAAAAAATTGTTTAGA
AAAATTGTTCGAAGAAGAAAATGCCACTAACTGGATAGGTTTACGGAAGC
TACAGGAACTTGATATATCTGACAATAAATTGACAGAACTCCCTGCCCTG
TTCCTTCACTCTTTCAAGTCCCTCAATTCTCTGAATGTCTCCAGAAACAA
CCTGAAGGTGTTTCCAGATCCCTGGGCCTGCCCTTTGAAATGTTGTAAAG
CTTCCAGAAATGCCCTGGAATGTCTGCCAGACAAAATGGCTGTCTTTTGG
AAAAATCACCTGAAGGATGTGGATTTCTCAGAAAACGCACTCAAAGAAGT
TCCCCTGGGACTTTTCCAGCTTGATGCCCTCATGTTCTTGAGGTTACAGG
GGAACCAGCTGGCGGCACTTCCACCTCAAGAGAAGTGGACCTGCAGGCAG
CTCAAAACCCTGGATCTCTCCAGAAACCAACTTGGCAAAAATGAAGATGG
ACTGAAAACGAAGCGTATTGCCTTTTTCACCACCAGAGGTCGCCAGCGCT
CCGGGACTGAGGCAGCAAGTGTGCTGGAATTTCCGGCCTTCCTAAGTGAG
TCTTTGGAAGTCCTTTGCCTGAACGACAACCACCTCGACACAGTCCCTCC
CTCGGTTTGCCTACTGAAGAGCTTATCAGAGCTCTACTTGGGAAACAACC
CTGGCCTCCGGGAGCTCCCTCCTGAGCTGGGGCAGCTGGGCAACCTCTGG
CAGCTGGACACTGAAGACCTGACCATCAGCAATGTGCCTGCAGAAATCCA
AAAAGAAGGCCCCAAAGCAATGCTGTCTTACCTGCGTGCTCAGCTGCGGA
AAGCGGAAAAGTGCAAGCTGATGAAGATGATCATCGTGGGTCCCCCGCGC
CAGGGCAAGTCCACCCTCCTGGAGATCTTACAGACGGGGAGGGCCCCCCA
GGTGGTGCATGGAGAGGCCACCATCAGGACCACCAAGTGGGAGCTCCAGA
GGCCGGCTGGCTCAAGAGCCAAGGTTGAGTCCGTGGAGTTCAACGTCTGG
GACATCGGGGGACCGGCCAGCATGGCCACTGTCAACCAGTGCTTCTTCAC
GGACAAGGCCCTGTACGTGGTGGTCTGGAACCTGGCGCTGGGGGAGGAGG
CCGTGGCCAACCTCCAGTTCTGGCTGCTCAACATCGAGGCCggGGCCCCA
AACGCCGTGGTGCTGGTGGTCGGGACGCACCTGGATTTAATTGAAGCCAA
GTTCCGTGTGGAAAGGATTGCAACGCTGCGTGCCTATGTGCTGGCACTCT
GCCGCTCCCCCTCCGGCTCCAGGGCCACAGGCTTCCCAGACATCACCTTC
AAACACTTACATGAGATTTCCTGCAAGAGCCTGGAAGGTCAGGAAGGGCT
GCGACAGCTGATTTTCCACGTCACGTGCAGCATGAAGGACGTGGGCAGCA
CCATCGGCTGCCAGCGACTGGCAGGGCGGCTGATCCCCAGGAGCTACCTG
AGCCTGCAGGAGGCCGTGCTGGCAGAGCAGCAGCGCCGCAGCCGGGACGA
CGACGTGCAGTACCTGACGGACAGGCAGCTGGAGCAGCTGGTGGAGCAGA
CGCCCGACAACGACATCAAGGACTACGAGGACCTGCAGTCAGCCATCAGC
TTCCTCATAGAAACCGGCACCCTGCTCCATTTCCCGGACACCAGCCACGG
CCTGAGGAACCTCTACTTCCTCGACCCTATTTGGCTCTCCGAATGTCTGC
AGAGGATCTTTAATATTAAGGGCTCTCGGTCAGTGGCCAAGAATGGGGTG
ATCAGAGCAGAAGACCTCAGGATGCTGCTGGTGGGGACTGGCTTCACGCA
GCAGACGGAAGAGCAGTACTTCCAGTTCCTGGCCAAGTTTGAGATCGCCC
TGCCCGTCGCCAATGACAGCTACCTCCTGCCCCATCTCCTTCCATCTAAA
CCTGGCCTGGACACCCACGGTATGCGGCACCCCACAGCCAACACCATTCA
GAGGGTATTTAAGATGAGCTTCGTTCCCGTTGGCTTCTGGCAAAGGTTTA
TAGCACGGATGCTGATCAGCCTGGCGGAGATGGACCTGCAGCTTTTTGAA
AACAAGAAGAATACTAAAAGCAGGAACAGGAAAGTCACCATTTACAGTTT
TACAGGAAACCAGAGAAATCGCTGTAGCACATTCAGAGTGAAAAGAAATC
AGACCATCTATTGGCAGGAAGGGCTCCTGGTCACTTTTGATGGGGGCTAC
CTCAGTGTGGAATCTTCCGACGTGAACTGGAAAAAGAAGAAAAGCGGAGG
AATGAAAATTGTTTGCCAATCAGAAGTGAGGGACTTCTCAGCCATGGCTT
TCATCACGGACCACGTCAATTCCTTGATTGATCAGTGGTTTCCCGCCCTG
ACAGCCACAGAGAGCGACGGGACGCCACTCATGGAGCAGTACGTGCCCTG
CCCGGTCTGCGAGACAGCCTGGGCCCAGCACACGGACCCCAGTGAGAAAT
CAGAGGATGTGCAGTACTTCGACATGGAAGACTGTGTCCTGACGGCCATC
GAGCGGGACTTCATCTCCTGCCCCAGACACCCGGACCTCCCCGTGCCGCT
GCAGGAGCTGGTCCCTGAACTGTTCATGACCGACTTCCCGGCCAGGCTCT
TCCTGGAGAACAGCAAGCTGGAGCACAGCGAGGACGAGGGCAGCGTCCTG
GGCCAGGGCGGCAGTGGCACCGTCATCTACCGGGCCCGGTACCAGGGCCA
GCCTGTGGCCGTCAAGCGCTTCCACATCAAAAAATTCAAGAACTTTGCTA
ACGTACCGGCAGACACCATGCTGAGGCACCTGCGGGCCACCGATGCCATG
AAGAACTTCTCCGAGTTCCGGCAGGAGGCCAGCATGCTGCACGCGCTGCA
GCACCCCTGCATCGTGGCGCTCATCGGCATCAGCATCCACCCGCTCTGCT
TCGCCCTGGAGCTCGCGCCGCTCAGCAGCCTCAACACCGTGCTGTCCGAG
AACGCCAGAGATTCTTCCTTTATACCCCTGGGACACATGCTCACCCAAAA
AATAGCCTACCAGATCGCCTCGGGCCTGGCCTACCTGCACAAGAAAAACA
TCATCTTCTGTGACCTGAAGTCGGACAACATTCTGGTGTGGTCCCTTGAC
GTCAAGGAGCACATCAACATCAAGCTATCTGcCTACGGGATTTCGAGGCA
GTCATTCCATGAGGGCGCCCTAGGCGTGGAGGGCACTCCTGGCTACCAGG
CCCCAGAGATCAGGCCTCGCATTGTATATGATGAGAAGGTAGATATGTTC
TCCTATGGAATGGTGCTCTACGAGTTGCTGTCAGGACAGCGCCCTGCACT
GGGCCACCACCAGCTCCAGATTGCCAAGAAGCTGTCCAAGGGCATCCGCC
CGGTTCTGGGGCAGCCGGAGGAAGTGCAGTTCCGGCGACTGCAGGCGCTC
ATGATGGAGTGCTGGGACACTAAGCCAGAGAAGCGACCGCTGGCCCTGTC
GGTGGTGAGCCAGATGAAGGACCCGACTTTTGCCACCTTCATGTATGAAC
TGTGCTGTGGGAAGCAGACAGCCTTCTTCTCATCCCAGGGCCAGGAGTAC
ACCGTGGTGTTTTGGGATGGAAAAGAGGAGTCCAGGAACTACACGGTGGT
GAACACAGAGAAGGGCCTCATGGAGGTGCAGAGGATGTGCTGCCCTGGGA
TGAAGGTGAGCTGCCAGCTCCAGGTCCAGAGATCCCTGTGGACAGCCACC
GAGGACCAGAAAATCTACATCTACACCCTCAAGGGCATGTGCCCCTTAAA
CACACCCCAACAGGCCTTGGATACTCCAGCTGTCGTCACCTGCTTCTTGG
CCGTGCCTGTTATTAAAAAGAATTCCTACCTGGTCTTAGCGGGCCTCGCC
GATGGGCTTGTGGCTGTGTTTCCCGTGGTGCGGGGCACCCCAAAGGACAG
CTGCTCCTACCTGTGCTCACACACAGCCAACAGGTCCAAGTTCAGCATCG
CGGATGAAGACGCACGGCAGAACCCCTACCCAGTGAAGGCCATGGAGGTG
GTCAACAGCGGCTCTGAGGTCTGGTACAGCAATGGGCCGGGCCTCCTTGT
CATCGACTGTGCCTCCCTGGAGATCTGCAGGCGGCTGGAGCCCTACATGG
CCCCCTCCATGGTTACGTCAGTCGTGTGCAGCTCTGAGGGCAGAGGGGAG
GAGGTCGTCTGGTGCCTGGATGACAAGGCCAACTCCTTGGTGATGTACCA
CTCCACCACCTACCAGCTGTGTGCCCGGTACTTCTGCGGGGTCCCCAGCC
CCCTCAGGGACATGTTTCCCGTGCGGCCCTTGGACACGGAACCCCCGGCA
GCCAGCCACACGGCCAACCCAAAGGTGCCTGAGGGGGACTCCATCGCGGA
CGTGAGCATCATGTACAGTGAGGAGCTGGGCACGCAGATCCTGATCCACC
AGGAATCACTCACTGACTACTGCTCCATGTCCTCCTACTCCTCATCCCCA
CCCCGCCAGGCTGCCAGGTCCCCCTCAAGCCTCCCCAGCTCCCCAGCAAG
TTCTTCCAGTGTGCCTTTCTCCACCGACTGCGAGGACTCAGACATGCTAC
ATACGCCCGGTGCTGCCTCCGACAGGTCTGAGCATGACCTGACCCCCATG
GACGGGGAGACCTTCAGCCAGCACCTGCAGGCCGTGAAGATCCTCGCCGT
CAGAGACCTCATTTGGGTCCCCAGGCGCGGTGGAGATGTTATCGTCATTG
GCCTGGAGAAGGATTCTGGCGCCCAGCGGGGCCGAGTCATTGCCGTCTTA
AAAGCCCGAGAGCTGACTCCGCATGGGGTGCTGGTGGATGCTGCCGTGGT
GGCAAAGGACACTGTTGTGTGCACCTTTGAAAATGAAAACACAGAGTGGT
GCCTGGCCGTCTGGAGGGGCTGGGGCGCCAGGGAGTTCGACATTTTCTAC
CAGTCCTACGAGGAGCTGGGCCGGCTGGAGGCTTGCACTCGCAAGAGAAG
GTAAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMAGMSQ
RPPSMYWCVGPEESAVCPERAMETLNGAGDTGGKPSTRGGDPAARSRRTE
GIRAAYRRGDRGGARDLLEEACDQCASQLEKGQLLSIPAAYGDLEMVRYL
LSKRLVELPTEPTDDNPAVVAAYFGHTAVVQELLESLPGPCSPQRLLNWM
LALACQRGHLGVVKLLVLTHGADPESYAVRKNEFPVIVRLPLYAAIKSGN
EDIAIFLLRHGAYFCSYILLDSPDPSKHLLRKYFIEASPLPSSYPGKTAL
RVKWSHLRLPWVDLDWLIDISCQITELDLSANCLATLPSVIPWGLINLRK
LNLSDNHLGELPGVQSSDEIICSRLLEIDISSNKLSHLPPGFLHLSKLQK
LTASKNCLEKLFEEENATNWIGLRKLQELDISDNKLTELPALFLHSFKSL
NSLNVSRNNLKVFPDPWACPLKCCKASRNALECLPDKMAVFWKNHLKDVD
FSENALKEVPLGLFQLDALMFLRLQGNQLAALPPQEKWTCRQLKTLDLSR
NQLGKNEDGLKTKRIAFFTTRGRQRSGTEAASVLEFPAFLSESLEVLCLN
DNHLDTVPPSVCLLKSLSELYLGNNPGLRELPPELGQLGNLWQLDTEDLT
ISNVPAEIQKEGPKAMLSYLRAQLRKAEKCKLMKMIIVGPPRQGKSTLLE
ILQTGRAPQVVHGEATIRTTKWELQRPAGSRAKVESVEFNVWDIGGPASM
ATVNQCFFTDKALYVVVWNLALGEEAVANLQFWLLNIEAGAPNAVVLVVG
THLDLIEAKFRVERIATLRAYVLALCRSPSGSRATGFPDITFKHLHEISC
KSLEGQEGLRQLIFHVTCSMKDVGSTIGCQRLAGRLIPRSYLSLQEAVLA
EQQRRSRDDDVQYLTDRQLEQLVEQTPDNDIKDYEDLQSAISFLIETGTL
LHFPDTSHGLRNLYFLDPIWLSECLQRIFNIKGSRSVAKNGVIRAEDLRM
LLVGTGFTQQTEEQYFQFLAKFEIALPVANDSYLLPHLLPSKPGLDTHGM
RHPTANTIQRVFKMSFVPVGFWQRFIARMLISLAEMDLQLFENKKNTKSR
NRKVTIYSFTGNQRNRCSTFRVKRNQTIYWQEGLLVTFDGGYLSVESSDV
NWKKKKSGGMKIVCQSEVRDFSAMAFITDHVNSLIDQWFPALTATESDGT
PLMEQYVPCPVCETAWAQHTDPSEKSEDVQYFDMEDCVLTAIERDFISCP
RHPDLPVPLQELVPELFMTDFPARLFLENSKLEHSEDEGSVLGQGGSGTV
IYRARYQGQPVAVKRFHIKKFKNFANVPADTMLRHLRATDAMKNFSEFRQ
EASMLHALQHPCIVALIGISIHPLCFALELAPLSSLNTVLSENARDSSFI
PLGHMLTQKIAYQIASGLAYLHKKNIIFCDLKSDNILVWSLDVKEHINIK
LSAYGISRQSFHEGALGVEGTPGYQAPEIRPRIVYDEKVDMFSYGMVLYE
LLSGQRPALGHHQLQIAKKLSKGIRPVLGQPEEVQFRRLQALMMECWDTK
PEKRPLALSVVSQMKDPTFATFMYELCCGKQTAFFSSQGQEYTVVFWDGK
EESRNYTVVNTEKGLMEVQRMCCPGMKVSCQLQVQRSLWTATEDQKIYIY
TLKGMCPLNTPQQALDTPAVVTCFLAVPVIKKNSYLVLAGLADGLVAVFP
VVRGTPKDSCSYLCSHTANRSKFSIADEDARQNPYPVKAMEVVNSGSEVW
YSNGPGLLVIDCASLEICRRLEPYMAPSMVTSVVCSSEGRGEEVVWCLDD
KANSLVMYHSTTYQLCARYFCGVPSPLRDMFPVRPLDTEPPAASHTANPK
VPEGDSIADVSIMYSEELGTQILIHQESLTDYCSMSSYSSSPPRQAARSP
SSLPSSPASSSSVPFSTDCEDSDMLHTPGAASDRSEHDLTPMDGETFSQH
LQAVKILAVRDLIWVPRRGGDVIVIGLEKDSGAQRGRVIAVLKARELTPH
GVLVDAAVVAKDTVVCTFENENTEWCLAVWRGWGAREFDIFYQSYEELGR
LEACTRKRR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU65288
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 13156
• Low Temp: No