cDNA Clone Malin

• Synonyms: NHLRC1
• Plasmid: pET28a-HALO-TEV-Malin Ala113-Gly395 (delta F215-D233)
• Parent Plasmid: pET28a-HALO-TEV
• Expressed: Halo-Malin[113-395] (delta F215-D233)
• Genbank: NM_198586
• Tag: HALO
• Species: Human

Sequence of Insert

ggatccGCCCTCACCTGCCACCACACCTTCGGCGGCTGGGGGACCCTGGT
CAACCCCACCGGACTGGCGCTTTGTCCCAAGACGGGGCGTGTCGTGGTGG
TGCACGACGGCAGGAGGCGTGTCAAGATTTTTGACTCAGGGGGAGGATGC
GCGCATCAGTTTGGAGAGAAGGGGGACGCTGCCCAAGACATTAGGTACCC
TGTGGATGTCACCATCACCAACGACTGCCATGTGGTTGTCACTGACGCCG
GCGATCGCTCCATCAAAGTGTTTGATTTTTTTGGCCAGATCAAGCTTGTC
ATTGGAGGCCAAGCGGAGGCAGGGTCCCTGCACCTCCTGGACGTCGACTT
CGCGGAAGGGGTCCTTCGGAGAACTGAAAGGTTGCAAGCTCATCTGTGCA
ATCCCCGAGGGGTGGCAGTGTCTTGGCTCACCGGGGCCATTGCGGTCCTG
GAGCACCCCCTGGCCCTGGGGACTGGGGTTTGCAGCACCAGGGTGAAAGT
GTTTAGCTCAAGTATGCAGCTTGTCGGCCAAGTGGATACCTTTGGGCTGA
GCCTCTACTTTCCCTCCAAAATAACTGCCTCCGCTGTGACCTTTGATCAC
CAGGGAAATGTGATTGTTGCAGATACATCTGGTCCAGCTATCCTTTGCTT
AGGAAAACCTGAGGAGTTTCCAGTACCGAAGCCCATGGTCACTCATGGTC
TTTCGCATCCTGTGGCTCTTACCTTCACCAAGGAGAATTCTCTTCTTGTG
CTGGACACAGCATCTCATTCTATAAAAGTCTATAAAGTTGACTGGGGGTG
Agcggccgc

Amino Acid Sequence

MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRN
IIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEV
VLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQ
AFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDRE
PLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPA
EAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTLEISGENL
YFQGGSALTCHHTFGGWGTLVNPTGLALCPKTGRVVVVHDGRRRVKIFDS
GGGCAHQFGEKGDAAQDIRYPVDVTITNDCHVVVTDAGDRSIKVFDFFGQ
IKLVIGGQAEAGSLHLLDVDFAEGVLRRTERLQAHLCNPRGVAVSWLTGA
IAVLEHPLALGTGVCSTRVKVFSSSMQLVGQVDTFGLSLYFPSKITASAV
TFDHQGNVIVADTSGPAILCLGKPEEFPVPKPMVTHGLSHPVALTFTKEN
SLLVLDTASHSIKVYKVDWG*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU65326
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 23222 (no 6His as Halo tag in vector cloned into Nco1 site, thereby deleting the 6His from pEt28a backbone)
• Low Temp: No