cDNA Clone
Laforin

Synonyms
EPM2, MELF, EPM2A
Plasmid
pcDNA5 FRT/TO-V5-laforin C266S
Parent Plasmid
pcDNA5 FRT/TO
Expressed
V5-Laforin C266S
Genbank
NM_005670
Tag
V5
Species
Human
Sequence of Insert
AGATCTATGGGCAAGCCCATCCCCAACCCCCTGCTGGGCCTGGACAGCAC
CggatccATGCGCTTCCGCTTTGGGGTGGTGGTGCCACCCGCCGTGGCCG
GCGCCCGGCCGGAGCTGCTGGTGGTGGGGTCGCGGCCCGAGCTGGGGCGT
TGGGAGCCGCGCGGTGCCGTCCGCCTGAGGCCGGCCGGCACCGCGGCGGG
CGACGGGGCCCTGGCGCTGCAGGAGCCGGGCCTGTGGCTCGGGGAGGTGG
AGCTGGCGGCCGAGGAGGCGGCGCAGGACGGGGCGGAGCCGGGCCGCGTG
GACACGTTCTGGTACAAGTTCCTGAAGCGGGAGCCGGGAGGAGAGCTCTC
CTGGGAAGGCAATGGACCTCATCATGACCGTTGCTGTACTTACAATGAAA
ACAACTTGGTGGATGGTGTGTATTGTCTCCCAATAGGACACTGGATTGAG
GCCACTGGACACACCAATGAAATGAAGCACACAACAGACTTCTATTTTAA
TATTGCAGGCCACCAAGCCATGCATTATTCAAGAATTCTACCAAATATCT
GGCTGGGTAGCTGCCCTCGTCAGGTGGAACATGTAACCATCAAACTGAAG
CATGAATTGGGGATTACAGCTGTAATGAATTTCCAGACTGAATGGGATAT
TGTACAGAATTCCTCAGGCTGTAACCGCTACCCAGAGCCCATGACTCCAG
ACACTATGATTAAACTATATAGGGAAGAAGGCTTGGCCTACATCTGGATG
CCAACACCAGATATGAGCACCGAAGGCCGAGTACAGATGCTGCCCCAGGC
GGTGTGCCTGCTGCATGCGCTGCTGGAGAAGGGACACATCGTGTACGTGC
ACTcCAACGCTGGGGTGGGCCGCTCCACAGCGGCTGTCTGCGGCTGGCTC
CAGTATGTGATGGGCTGGAATCTGAGGAAGGTGCAGTATTTCCTCATGGC
CAAGAGGCCGGCTGTCTACATTGACGAAGAGGCCTTGGCCCGGGCACAAG
AAGATTTTTTCCAGAAATTTGGGAAGGTTCGTTCTTCTGTGTGTAGCCTG
TAGgcggccgc
Amino Acid Sequence
MGKPIPNPLLGLDSTGSMRFRFGVVVPPAVAGARPELLVVGSRPELGRWE
PRGAVRLRPAGTAAGDGALALQEPGLWLGEVELAAEEAAQDGAEPGRVDT
FWYKFLKREPGGELSWEGNGPHHDRCCTYNENNLVDGVYCLPIGHWIEAT
GHTNEMKHTTDFYFNIAGHQAMHYSRILPNIWLGSCPRQVEHVTIKLKHE
LGITAVMNFQTEWDIVQNSSGCNRYPEPMTPDTMIKLYREEGLAYIWMPT
PDMSTEGRVQMLPQAVCLLHALLEKGHIVYVHSNAGVGRSTAAVCGWLQY
VMGWNLRKVQYFLMAKRPAVYIDEEALARAQEDFFQKFGKVRSSVCSL*
Vector Type
Mammalian
Antibiotic
Amp
DU Number
DU65345
Unit Source
Protein Phosphorylation and Ubiquitylation Unit
Location
PPU
MTA
Dundee
Notes
Parental 41459
Insert comprises BglII-V5 tag-BamHI-ORF-Not1, ORF can be excised without tag Bam-Not
ORF contains 3 silent changes compared NCBI ref:
Ala53 (GCC > GCG)
Gly134 (GGG > GGA)
Thr274 (ACC > ACA)
Low Temp
No
Price
£125.00
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