cDNA Clone Malin

• Plasmid: pET24a-C-(TEV)-Malin-C-DAC
• Parent Plasmid: pET24a-C-(TEV)-DAC
• Expressed: Malin-DAC
• Genbank: NM_198586.2
• Tag: DAC
• Species: Human

Sequence of Insert

GGATCCATGGCGGCCGAAGCCTCGGAGAGCGGGCCAGCGCTGCATGAGCTCATGCGCGAGGCGGAGATCAGCCTGCTCGAGTGCAAGGTGTGCTTTGAGAAGTTTGGCCACCGGCAGCAGCGGCGCCCGCGCAACCTGTCCTGCGGCCACGTGGTCTGCCTGGCCTGCGTGGCCGCCCTGGCGCACCCGCGCACTCTGGCCCTCGAGTGCCCATTCTGCAGGCGAGCTTGCCGGGGCTGCGACACCAGCGACTGCCTGCCGGTGCTGCACCTCATAGAGCTCCTGGGCTCAGCGCTTCGCCAGTCCCCGGCCGCCCATCGCGCCGCCCCCAGCGCCCCCGGAGCCCTCACCTGCCACCACACCTTCGGCGGCTGGGGGACCCTGGTCAACCCCACCGGACTGGCGCTTTGTCCCAAGACGGGGCGTGTCGTGGTGGTGCACGACGGCAGGAGGCGTGTCAAGATTTTTGACTCAGGGGGAGGATGCGCGCATCAGTTTGGAGAGAAGGGGGACGCTGCCCAAGACATTAGGTACCCTGTGGATGTCACCATCACCAACGACTGCCATGTGGTTGTCACTGACGCCGGCGATCGCTCCATCAAAGTGTTTGATTTTTTTGGCCAGATCAAGCTTGTCATTGGAGGCCAATTCTCCTTACCTTGGGGTGTGGAGACCACCCCTCAGAATGGGATTGTGGTAACTGATGCGGAGGCAGGGTCCCTGCACCTCCTGGACGTCGACTTCGCGGAAGGGGTCCTTCGGAGAACTGAAAGGTTGCAAGCTCATCTGTGCAATCCCCGAGGGGTGGCAGTGTCTTGGCTCACCGGGGCCATTGCGGTCCTGGAGCACCCCCTGGCCCTGGGGACTGGGGTTTGCAGCACCAGGGTGAAAGTGTTTAGCTCAAGTATGCAGCTTGTCGGCCAAGTGGATACCTTTGGGCTGAGCCTCTACTTTCCCTCCAAAATAACTGCCTCCGCTGTGACCTTTGATCACCAGGGAAATGTGATTGTTGCAGATACATCTGGTCCAGCTATCCTTTGCTTAGGAAAACCTGAGGAGTTTCCAGTACCGAAGCCCATGGTCACTCATGGTCTTTCGCATCCTGTGGCTCTTACCTTCACCAAGGAGAATTCTCTTCTTGTGCTGGACACAGCATCTCATTCTATAAAAGTCTATAAAGTTGACTGGGGGGCGGCCGC

Amino Acid Sequence

MAAEASESGPALHELMREAEISLLECKVCFEKFGHRQQRRPRNLSCGHVV
CLACVAALAHPRTLALECPFCRRACRGCDTSDCLPVLHLIELLGSALRQS
PAAHRAAPSAPGALTCHHTFGGWGTLVNPTGLALCPKTGRVVVVHDGRRR
VKIFDSGGGCAHQFGEKGDAAQDIRYPVDVTITNDCHVVVTDAGDRSIKV
FDFFGQIKLVIGGQFSLPWGVETTPQNGIVVTDAEAGSLHLLDVDFAEGV
LRRTERLQAHLCNPRGVAVSWLTGAIAVLEHPLALGTGVCSTRVKVFSSS
MQLVGQVDTFGLSLYFPSKITASAVTFDHQGNVIVADTSGPAILCLGKPE
EFPVPKPMVTHGLSHPVALTFTKENSLLVLDTASHSIKVYKVDWGAAALE
ENLYFQGMSAIPGVPQIDAESYILIDYNSGKVLAEQNADVRRDPASLTKM
MTSYVIGQAMKAGKFKETDLVTIGNDAWATGNPVFKGSSLMFLKPGMQVP
VSQLIRGINLQSGNDACVAMADFAAGSQDAFVGLMNSYVNALGLKNTHFQ
TVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFTFNGIRQLNR
NGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKGR
EAESKKLLTWGFRFFETVNP*

• Vector Type: Bacterial
• Antibiotic: Kan
• Full Snapgene Map: pET24a-C-(TEV)-Malin-C-DAC 78.34 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU65355
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 65346; NB Nde1 site immediately upstream of 5' BamHI cloning site gives an additional 3 resides N-terminal to the Malin initiation (MGS)
• Low Temp: No