cDNA Clone Malin

• Plasmid: pET24a-C-(TEV)-Malin F33S-C-DAC
• Parent Plasmid: pET24a-C-(TEV)-DAC
• Expressed: Malin F33S-DAC
• Genbank: NM_198586.2
• Tag: DAC
• Species: Human

Sequence of Insert

GGATCCATGGCGGCCGAAGCCTCGGAGAGCGGGCCAGCGCTGCATGAGCT
CATGCGCGAGGCGGAGATCAGCCTGCTCGAGTGCAAGGTGTGCTTTGAGA
AGTcTGGCCACCGGCAGCAGCGGCGCCCGCGCAACCTGTCCTGCGGCCAC
GTGGTCTGCCTGGCCTGCGTGGCCGCCCTGGCGCACCCGCGCACTCTGGC
CCTCGAGTGCCCATTCTGCAGGCGAGCTTGCCGGGGCTGCGACACCAGCG
ACTGCCTGCCGGTGCTGCACCTCATAGAGCTCCTGGGCTCAGCGCTTCGC
CAGTCCCCGGCCGCCCATCGCGCCGCCCCCAGCGCCCCCGGAGCCCTCAC
CTGCCACCACACCTTCGGCGGCTGGGGGACCCTGGTCAACCCCACCGGAC
TGGCGCTTTGTCCCAAGACGGGGCGTGTCGTGGTGGTGCACGACGGCAGG
AGGCGTGTCAAGATTTTTGACTCAGGGGGAGGATGCGCGCATCAGTTTGG
AGAGAAGGGGGACGCTGCCCAAGACATTAGGTACCCTGTGGATGTCACCA
TCACCAACGACTGCCATGTGGTTGTCACTGACGCCGGCGATCGCTCCATC
AAAGTGTTTGATTTTTTTGGCCAGATCAAGCTTGTCATTGGAGGCCAATT
CTCCTTACCTTGGGGTGTGGAGACCACCCCTCAGAATGGGATTGTGGTAA
CTGATGCGGAGGCAGGGTCCCTGCACCTCCTGGACGTCGACTTCGCGGAA
GGGGTCCTTCGGAGAACTGAAAGGTTGCAAGCTCATCTGTGCAATCCCCG
AGGGGTGGCAGTGTCTTGGCTCACCGGGGCCATTGCGGTCCTGGAGCACC
CCCTGGCCCTGGGGACTGGGGTTTGCAGCACCAGGGTGAAAGTGTTTAGC
TCAAGTATGCAGCTTGTCGGCCAAGTGGATACCTTTGGGCTGAGCCTCTA
CTTTCCCTCCAAAATAACTGCCTCCGCTGTGACCTTTGATCACCAGGGAA
ATGTGATTGTTGCAGATACATCTGGTCCAGCTATCCTTTGCTTAGGAAAA
CCTGAGGAGTTTCCAGTACCGAAGCCCATGGTCACTCATGGTCTTTCGCA
TCCTGTGGCTCTTACCTTCACCAAGGAGAATTCTCTTCTTGTGCTGGACA
CAGCATCTCATTCTATAAAAGTCTATAAAGTTGACTGGGGGGCGGCCGC

Amino Acid Sequence

MGSMAAEASESGPALHELMREAEISLLECKVCFEKSGHRQQRRPRNLSCG
HVVCLACVAALAHPRTLALECPFCRRACRGCDTSDCLPVLHLIELLGSAL
RQSPAAHRAAPSAPGALTCHHTFGGWGTLVNPTGLALCPKTGRVVVVHDG
RRRVKIFDSGGGCAHQFGEKGDAAQDIRYPVDVTITNDCHVVVTDAGDRS
IKVFDFFGQIKLVIGGQFSLPWGVETTPQNGIVVTDAEAGSLHLLDVDFA
EGVLRRTERLQAHLCNPRGVAVSWLTGAIAVLEHPLALGTGVCSTRVKVF
SSSMQLVGQVDTFGLSLYFPSKITASAVTFDHQGNVIVADTSGPAILCLG
KPEEFPVPKPMVTHGLSHPVALTFTKENSLLVLDTASHSIKVYKVDWGAA
ALEENLYFQGMSAIPGVPQIDAESYILIDYNSGKVLAEQNADVRRDPASL
TKMMTSYVIGQAMKAGKFKETDLVTIGNDAWATGNPVFKGSSLMFLKPGM
QVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVGLMNSYVNALGLKNT
HFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFTFNGIRQ
LNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTF
KGREAESKKLLTWGFRFFETVNP*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU65356
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 65346; NB Nde1 site immediately upstream of 5' BamHI cloning site gives an additional 3 resides N-terminal to the Malin initiation (MGS)
• Low Temp: No