cDNA Clone LATS1

• Synonyms: WARTS, wts
• Plasmid: pFastbacDual GST6P1 LATS1 S464A
• Parent Plasmid: pFastbacDual GST6P1
• Expressed: GST LATS1 S464A
• Genbank: NM_004690.3
• Tag: GST
• Species: Human

Sequence of Insert

GGATCTATGAAGAGGAGTGAAAAGCCAGAAGGATATAGACAAATGAGGCCTAAGACCTTTCCTGCCAGTAACTATACTGTCAGTAGCCGGCAAATGTTACAAGAAATTCGGGAATCCCTTAGGAATTTATCTAAACCATCTGATGCTGCTAAGGCTGAGCATAACATGAGTAAAATGTCAACCGAAGATCCTCGACAAGTCAGAAATCCACCCAAATTTGGGACGCATCATAAAGCCTTGCAGGAAATTCGAAACTCTCTGCTTCCATTTGCAAATGAAACAAATTCTTCTCGGAGTACTTCAGAAGTTAATCCACAAATGCTTCAAGACTTGCAAGCTGCTGGATTTGATGAGGATATGGTTATACAAGCTCTTCAGAAAACTAACAACAGAAGTATAGAAGCAGCAATTGAATTCATTAGTAAAATGAGTTACCAAGATCCTCGACGAGAGCAGATGGCTGCAGCAGCTGCCAGACCTATTAATGCCAGCATGAAACCAGGGAATGTGCAGCAATCAGTTAACCGCAAACAGAGCTGGAAAGGTTCTAAAGAATCCTTAGTTCCTCAGAGGCATGGCCCGCCACTAGGAGAAAGTGTGGCCTATCATTCTGAGAGTCCCAACTCACAGACAGATGTAGGAAGACCTTTGTCTGGATCTGGTATATCAGCATTTGTTCAAGCTCACCCTAGCAACGGACAGAGAGTGAACCCCCCACCACCACCTCAAGTAAGGAGTGTTACTCCTCCACCACCTCCAAGAGGCCAGACTCCCCCTCCAAGAGGTACAACTCCACCTCCCCCTTCATGGGAACCAAACTCTCAAACAAAGCGCTATTCTGGAAACATGGAATACGTAATCTCCCGAATCTCTCCTGTCCCACCTGGGGCATGGCAAGAGGGCTATCCTCCACCACCTCTCAACACTTCCCCCATGAATCCTCCTAATCAAGGACAGAGAGGCATTAGTTCTGTTCCTGTTGGCAGACAACCAATCATCATGCAGAGTTCTAGCAAATTTAACTTTCCATCAGGGAGACCTGGAATGCAGAATGGTACTGGACAAACTGATTTCATGATACACCAAAATGTTGTCCCTGCTGGCACTGTGAATCGGCAGCCACCACCTCCATATCCTCTGACAGCAGCTAATGGACAAAGCCCTTCTGCTTTACAAACAGGGGGATCTGCTGCTCCTTCGTCATATACAAATGGAAGTATTCCTCAGTCTATGATGGTGCCAAACAGAAATAGTCATAACATGGAACTATATAACATTAGTGTACCTGGACTGCAAACAAATTGGCCTCAGTCATCTTCTGCTCCAGCCCAGTCATCCCCGAGCAGTGGGCATGAAATCCCTACATGGCAACCTAACATACCAGTGAGGTCAAATGCTTTTAATAACCCATTAGGAAATAGAGCAAGTCACTCTGCTAATTCTCAGCCTTCTGCTACAACAGTCACTGCAATTACACCAGCTCCTATTCAACAGCCTGTGAAAAGTATGCGTGTATTAAAACCAGAGCTACAGACTGCTTTAGCACCTACACACCCTTCTTGGATACCACAGCCAATTCAAACTGTTCAACCCAGTCCTTTTCCTGAGGGAACCGCTTCAAATGTGACTGTGATGCCACCTGTTGCTGAAGCTCCAAACTATCAAGGACCACCACCACCCTACCCAAAACATCTGCTGCACCAAAACCCATCTGTTCCTCCATACGAGTCAATCAGTAAGCCTAGCAAAGAGGATCAGCCAAGCTTGCCCAAGGAAGATGAGAGTGAAAAGAGTTATGAAAATGTTGATAGTGGGGATAAAGAAAAGAAACAGATTACAACTTCACCTATTACTGTTAGGAAAAACAAGAAAGATGAAGAGCGAAGGGAATCTCGTATTCAAAGTTATTCTCCTCAAGCATTTAAATTCTTTATGGAGCAACATGTAGAAAATGTACTCAAATCTCATCAGCAGCGTCTACATCGTAAAAAACAATTAGAGAATGAAATGATGCGGGTTGGATTATCTCAAGATGCCCAGGATCAAATGAGAAAGATGCTTTGCCAAAAAGAATCTAATTACATCCGTCTTAAAAGGGCTAAAATGGACAAGTCTATGTTTGTGAAGATAAAGACACTAGGAATAGGAGCATTTGGTGAAGTCTGTCTAGCAAGAAAAGTAGATACTAAGGCTTTGTATGCAACAAAAACTCTTCGAAAGAAAGATGTTCTTCTTCGAAATCAAGTCGCTCATGTTAAGGCTGAGAGAGATATCCTGGCTGAAGCTGACAATGAATGGGTAGTTCGTCTATATTATTCATTCCAAGATAAGGACAATTTATACTTTGTAATGGACTACATTCCTGGGGGTGATATGATGAGCCTATTAATTAGAATGGGCATCTTTCCAGAAAGTCTGGCACGATTCTACATAGCAGAACTTACCTGTGCAGTTGAAAGTGTTCATAAAATGGGTTTTATTCATAGAGATATTAAACCTGATAATATTTTGATTGATCGTGATGGTCATATTAAATTGACTGACTTTGGCCTCTGCACTGGCTTCAGATGGACACACGATTCTAAGTACTATCAGAGTGGTGACCATCCACGGCAAGATAGCATGGATTTCAGTAATGAATGGGGGGATCCCTCAAGCTGTCGATGTGGAGACAGACTGAAGCCATTAGAGCGGAGAGCTGCACGCCAGCACCAGCGATGTCTAGCACATTCTTTGGTTGGGACTCCCAATTATATTGCACCTGAAGTGTTGCTACGAACAGGATACACACAGTTGTGTGATTGGTGGAGTGTTGGTGTTATTCTTTTTGAAATGTTGGTGGGACAACCTCCTTTCTTGGCACAAACACCATTAGAAACACAAATGAAGGTTATCAACTGGCAAACATCTCTTCACATTCCACCACAAGCTAAACTCAGTCCTGAAGCTTCTGATCTTATTATTAAACTTTGCCGAGGACCCGAAGATCGCTTAGGCAAGAATGGTGCTGATGAAATAAAAGCTCATCCATTTTTTAAAACAATTGACTTCTCCAGTGACCTGAGACAGCAGTCTGCTTCATACATTCCTAAAATCACACACCCAACAGATACATCAAATTTTGATCCTGTTGATCCTGATAAATTATGGAGTGATGATAACGAGGAAGAAAATGTAAATGACACTCTCAATGGATGGTATAAAAATGGAAAGCATCCTGAACATGCATTCTATGAATTTACCTTCCGAAGGTTTTTTGATGACAATGGCTACCCATATAATTATCCGAAGCCTATTGAATATGAATACATTAATTCACAAGGCTCAGAGCAGCAGTCGGATGAAGATGATCAAAACACAGGCTCAGAGATTAAAAATCGCGATCTAGTATATGTTTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMKRSEKPEGYRQMRPKTFPASNYTVSSRQMLQEIRESLRNLSKPSDAAKAEHNMSKMSTEDPRQVRNPPKFGTHHKALQEIRNSLLPFANETNSSRSTSEVNPQMLQDLQAAGFDEDMVIQALQKTNNRSIEAAIEFISKMSYQDPRREQMAAAAARPINASMKPGNVQQSVNRKQSWKGSKESLVPQRHGPPLGESVAYHSESPNSQTDVGRPLSGSGISAFVQAHPSNGQRVNPPPPPQVRSVTPPPPPRGQTPPPRGTTPPPPSWEPNSQTKRYSGNMEYVISRISPVPPGAWQEGYPPPPLNTSPMNPPNQGQRGISSVPVGRQPIIMQSSSKFNFPSGRPGMQNGTGQTDFMIHQNVVPAGTVNRQPPPPYPLTAANGQSPSALQTGGSAAPSSYTNGSIPQSMMVPNRNSHNMELYNISVPGLQTNWPQSSSAPAQSSPSSGHEIPTWQPNIPVRSNAFNNPLGNRASHSANSQPSATTVTAITPAPIQQPVKSMRVLKPELQTALAPTHPSWIPQPIQTVQPSPFPEGTASNVTVMPPVAEAPNYQGPPPPYPKHLLHQNPSVPPYESISKPSKEDQPSLPKEDESEKSYENVDSGDKEKKQITTSPITVRKNKKDEERRESRIQSYSPQAFKFFMEQHVENVLKSHQQRLHRKKQLENEMMRVGLSQDAQDQMRKMLCQKESNYIRLKRAKMDKSMFVKIKTLGIGAFGEVCLARKVDTKALYATKTLRKKDVLLRNQVAHVKAERDILAEADNEWVVRLYYSFQDKDNLYFVMDYIPGGDMMSLLIRMGIFPESLARFYIAELTCAVESVHKMGFIHRDIKPDNILIDRDGHIKLTDFGLCTGFRWTHDSKYYQSGDHPRQDSMDFSNEWGDPSSCRCGDRLKPLERRAARQHQRCLAHSLVGTPNYIAPEVLLRTGYTQLCDWWSVGVILFEMLVGQPPFLAQTPLETQMKVINWQTSLHIPPQAKLSPEASDLIIKLCRGPEDRLGKNGADEIKAHPFFKTIDFSSDLRQQSASYIPKITHPTDTSNFDPVDPDKLWSDDNEEENVNDTLNGWYKNGKHPEHAFYEFTFRRFFDDNGYPYNYPKPIEYEYINSQGSEQQSDEDDQNTGSEIKNRDLVYV*

• Vector Type: Baculovirus
• Antibiotic: Amp
• Full Snapgene Map: pFastbacDual GST6P1 LATS1 S464A66.98 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU68395
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Human LATS1 expressed as a recombinant protein with S464 mutated to Ala in Sf21 cells, for kinase assays. Thanks!
• Low Temp: No