cDNA Clone ZUFSP

• Plasmid: pGEX6P1 ZUFSP_HUMAN D186-I322 ZUFSP_SPOMBE S2-F244
• Parent Plasmid: pGEX6P1
• Expressed: pGEX6P1 ZUFSP_HUMAN D186-I322 ZUFSP_SPOMBE S2-F244
• Genbank: NM_145062.2 GI:292494918 and SPAC25H1.04
• Tag: GST
• Species: Human

Sequence of Insert

ATGGACTGTGATCAACCACTCTATGATTGTCCTATGTGTGGGCTCATATGTACAAATTACCATATTCTTCAGGAACATGTTGACTTGCATTTGGAAGAAAACAGCTTTCAGCAAGGCATGGATAGAGTCCAGTGTTCTGGTGATCTACAATTGGCTCACCAGCTTCAGCAAGAAGAAGACAGAAAGAGGAGATCTGAAGAATCAAGACAAGAAATAGAAGAATTTCAGAAGCTGCAGAGACAATATGGTTTAGATAATTCTGGAGGATACAAACAACAACAACTACGAAATATGGAGATAGAAGTAAATAGGGGAAGAATGCCTCCATCTGAATTTCATAGGAGAAAAGCTGATATGATGGAATCATTAGCTCTTGGTTTTGACGATGGAAAAACAAAAACTTCCGGAATT

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMDCDQPLYDCPMCGLICTNYHILQEHVDLHLEENSFQQGMDRVQCSGDLQLAHQLQQEEDRKRRSEESRQEIEEFQKLQRQYGLDNSGGYKQQQLRNMEIEVNRGRMPPSEFHRRKADMMESLALGFDDGKTKTSGISKCLQQLKRQLQHFGIDGCSLADGDIDYFFTVTGIDRGWGCGWRNIQMLISWLQYTNPNWFKRNFSSGNYEINSLQSLLLSAWMKGIDAEGYAQLGDNLHGKWIGATEVYSLFTGLFVNVALVDFDFRSEASASNALFLYVKKHFESSNDTSNVSPCYLQFQGHSIIIIGFCSSLETLVVLDPDRYQSVQKKFVNIADFNHCYMRKKRSLKFSQFQLVHFKQNIFLNDFSSKLEVRSTRISDF*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pGEX6P1 ZUFSP_HUMAN D186-I322 ZUFSP_SPOMBE S2-F244320.16 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU66320
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Could you please clone human ZUFSP 184-322 in fusion with yeast ZUFSP (DU55991) 2-244-----both Zufsps can be cut out together with B/N,; ; Generated by NEB builder, vector 55991 was amplified with SW5768/9 and insert was amplified from 55846 with SW5772/1
• Low Temp: No