cDNA Clone PRKAA1

• Synonyms: AMPK, AMPKa1
• Plasmid: pX459 PRKAA1 ex5 KO Sense G3
• Parent Plasmid: pX459
• Expressed: PRKAA1 ex5 G3 + spCas9 + puro
• Genbank: ENSG00000132356
• Species: Human

Sequence of Insert

GCTGTTCCAACAGATCCTTTC

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU64958
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: CRISPR KO guide. Single vector system expressing spCas9 and puro for selection. Test all 3 individually and then use the optimal guide in combination with the optimal PRKAA2 guide to obtain the double KO.
• Low Temp: No