cDNA Clone USP29

• Synonyms: HOM-TES-84/86
• Plasmid: pCAGGS puro 3FLAG USP29
• Parent Plasmid: pCAGGS puro 3FLAG
• Expressed: 3FLAG USP29
• Genbank: NM_020903.2
• Tag: 3FLAG
• Species: Human

Amino Acid Sequence

MDYKDHDGDYKDHDIDYKDDDDKMISLKVCGFIQIWSQKTGMTKLKEALIETVQRQKEIKLVVTFKSGKFIRIFQLSNNIRSVVLRHCKKRQSHLRLTLK
NNVFLFIDKLSYRDAKQLNMFLDIIHQNKSQQPMKSDDDWSVFESRNMLKEIDKTSFYSICNKPSYQKMPLFMSKSPTHVKKGILENQGGKGQNTLSSDV
QTNEDILKEDNPVPNKKYKTDSLKYIQSNRKNPSSLEDLEKDRDLKLGPSFNTNCNGNPNLDETVLATQTLNAKNGLTSPLEPEHSQGDPRCNKAQVPLD
SHSQQLQQGFPNLGNTCYMNAVLQSLFAIPSFADDLLTQGVPWEYIPFEALIMTLTQLLALKDFCSTKIKRELLGNVKKVISAVAEIFSGNMQNDAHEFL
GQCLDQLKEDMEKLNATLNTGKECGDENSSPQMHVGSAATKVFVCPVVANFEFELQLSLICKACGHAVLKVEPNNYLSINLHQETKPLPLSIQNSLDLFF
KEEELEYNCQMCKQKSCVARHTFSRLSRVLIIHLKRYSFNNAWLLVKNNEQVYIPKSLSLSSYCNESTKPPLPLSSSAPVGKCEVLEVSQEMISEINSPL
TPSMKLTSESSDSLVLPVEPDKNADLQRFQRDCGDASQEQHQRDLENGSALESELVHFRDRAIGEKELPVADSLMDQGDISLPVMYEDGGKLISSPDTRL
VEVHLQEVPQHPELQKYEKTNTFVEFNFDSVTESTNGFYDCKENRIPEGSQGMAEQLQQCIEESIIDEFLQQAPPPGVRKLDAQEHTEETLNQSTELRLQ
KADLNHLGALGSDNPGNKNILDAENTRGEAKELTRNVKMGDPLQAYRLISVVSHIGSSPNSGHYISDVYDFQKQAWFTYNDLCVSEISETKMQEARLHSG
YIFFYMHNGIFEELLRKAENSRLPSTQAGVIPQGEYEGDSLYRPA*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pCAGGS puro 3FLAG USP29106.39 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU61201
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Subcloned from DU49618 pcDNA5D FRT/TO GFP USP29 (Not1) into DU49056 pCAGGS puro 3FLAG (Not1). Mutagenesis of this clone to remove the extra sequence and stop codon to make final product using oligos JC256/JC257.
• Low Temp: No