cDNA Clone DYRK1A

• Synonyms: DYRK; DYRK1; HP86; MNB; MNBH; MRD7
• Plasmid: pcDNA5 FRT TO GFP DYRK1A M1-S502
• Parent Plasmid: pcDNA5 FRT TO GFP
• Expressed: GFP DYRK1A M1-S502
• Genbank: NP_569121 GenBank: U58496.1
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGATGCATACAGGAGGAGAGACTTCAGCATGCAAACCTTCATCTGTCCGGCTTGCACCGTCGTTCTCATTCCATGCTGCTGGCCTTCAGATGGCTGGACAGATGCCCCACTCACACCAGTACAGTGACCGTCGCCAGCCGAACATAAGTGACCAGCAGGTGTCTGCCTTATCATATTCTGACCAGATTCAGCAACCTCTAACTAACCAGGTGATGCCTGATATTGTCATGTTACAGAGGCGGATGCCCCAAACCTTCCGTGATCCAGCAACTGCTCCTCTGAGAAAACTCTCTGTGGACTTGATCAAAACATACAAGCATATTAATGAGGTTTACTATGCAAAAAAGAAGCGAAGACACCAACAGGGCCAGGGGGACGATTCCAGTCATAAGAAGGAGCGGAAGGTTTACAATGATGGTTACGATGATGATAACTATGATTATATTGTAAAAAACGGGGAAAAGTGGATGGATCGGTATGAAATCGACTCCTTAATAGGCAAAGGTTCATTTGGACAGGTTGTGAAAGCTTATGACAGAGTGGAGCAAGAATGGGTCGCCATTAAAATCATCAAGAACAAGAAAGCGTTTCTGAATCAAGCCCAGATAGAAGTGCGGCTGCTTGAGCTCATGAACAAACACGACACTGAAATGAAGTACTACATAGTGCATTTGAAACGCCACTTTATGTTTCGAAACCATCTCTGTTTAGTGTTTGAAATGCTGTCCTATAATCTCTATGATTTGTTGAGAAACACCAACTTCCGAGGGGTCTCTTTGAACCTAACACGAAAGTTTGCGCAACAGATGTGCACAGCATTGCTTTTTCTTGCGACTCCAGAACTTAGTATCATTCACTGTGACTTAAAGCCTGAAAATATCCTTCTTTGTAACCCCAAACGCAGTGCAATCAAGATAGTTGACTTTGGCAGTTCTTGTCAGTTGGGGCAGAGGATATACCAGTATATTCAGAGTCGCTTTTATCGGTCTCCAGAGGTGCTACTGGGAATGCCTTATGACCTTGCCATTGATATGTGGTCCCTCGGGTGTATTTTGGTTGAAATGCACACTGGAGAACCTCTGTTCAGTGGTGCCAATGAGGTAGATCAGATGAATAAAATAGTGGAAGTTCTGGGTATTCCACCTGCTCATATTCTTGACCAAGCACCAAAAGCAAGAAAGTTCTTTGAGAAGTTGCCAGATGGCACTTGGAACTTAAAGAAGACCAAAGATGGAAAACGGGAGTACAAACCACCAGGAACCCGTAAACTTCATAACATTCTTGGAGTGGAAACAGGAGGACCTGGTGGGCGACGTGCTGGGGAGTCAGGTCATACGGTCGCTGACTACTTGAAGTTCAAAGACCTCATTTTAAGGATGCTTGATTATGACCCCAAAACTCGAATTCAACCTTATTATGCTCTGCAGCACAGTTTCTTCAAGAAAACAGCTGATGAAGGTACAAATACAAGTAATAGTGTATCTACAAGCCCCGCCATGGAGCAGTCTTAAGCGCCGCC

Amino Acid Sequence

MHTGGETSACKPSSVRLAPSFSFHAAGLQMAGQMPHSHQYSDRRQPNISDQQVSALSYSDQIQQPLTNQVMPDIVMLQRRMPQTFRDPATAPLRKLSVDLIKTYKHINEVYYAKKKRRHQQGQGDDSSHKKERKVYNDGYDDDNYDYIVKNGEKWMDRYEIDSLIGKGSFGQVVKAYDRVEQEWVAIKIIKNKKAFLNQAQIEVRLLELMNKHDTEMKYYIVHLKRHFMFRNHLCLVFEMLSYNLYDLLRNTNFRGVSLNLTRKFAQQMCTALLFLATPELSIIHCDLKPENILLCNPKRSAIKIVDFGSSCQLGQRIYQYIQSRFYRSPEVLLGMPYDLAIDMWSLGCILVEMHTGEPLFSGANEVDQMNKIVEVLGIPPAHILDQAPKARKFFEKLPDGTWNLKKTKDGKREYKPPGTRKLHNILGVETGGPGGRRAGESGHTVADYLKFKDLILRMLDYDPKTRIQPYYALQHSFFKKTADEGTNTSNSVSTSPAMEQS*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pcDNA5 FRT TO GFP DYRK1A M1-S50288 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU66331
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: silent changes compared to reference NM_001396.4, Protein sequence matches NP_001387.2 GI:18765758. Nucleotide sequence humanised from mouse. Direct digest of PCR product
• Low Temp: No