cDNA Clone IRAK4

• Plasmid: pFastbac 6HIS TEV GST 3C-IRAK4 aa160-460
• Parent Plasmid: pFastbac 6HIS TEV GST
• Expressed: 6His-TEV-GST-3C-IRAK4 aa160-460
• Genbank: NM_001114182
• Tag: GST
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCGTTAGTGATACACGTTTTCACAGTTTTTCATTTTATGAATTGAA GAATGTCACAAATAACTTTGATGAACGACCCATTTCTGTTGGTGGTAATA AAATGGGAGAGGGAGGATTTGGAGTTGTATATAAAGGCTACGTAAATAAC ACAACTGTGGCAGTGAAGAAGCTTGCAGCAATGGTTGACATTACTACTGA AGAACTGAAACAGCAGTTTGATCAAGAAATAAAAGTAATGGCAAAGTGTC AACATGAAAACTTAGTAGAACTACTTGGTTTCTCAAGTGATGGAGATGAC CTCTGCTTAGTATATGTTTACATGCCTAATGGTTCATTGCTAGACAGACT CTCTTGCTTGGATGGTACTCCACCACTTTCTTGGCACATGAGATGCAAGA TTGCTCAGGGTGCAGCTAATGGCATCAATTTTCTACATGAAAATCATCAT ATTCATAGAGATATTAAAAGTGCAAATATCTTACTGGATGAAGCTTTTAC TGCTAAAATATCTGACTTTGGCCTTGCACGGGCTTCTGAGAAGTTTGCCC AGACAGTCATGACTAGCAGAATTGTGGGAACAACAGCTTATATGGCACCA GAAGCTTTGCGTGGAGAAATAACACCCAAATCTGATATTTACAGCTTTGG TGTGGTTTTACTAGAAATAATAACTGGACTTCCAGCTGTGGATGAACACC GTGAACCTCAGTTATTGCTAGATATTAAAGAAGAAATTGAAGATGAAGAA AAGACAATTGAAGATTATATTGATAAAAAGATGAATGATGCTGATTCCAC TTCAGTTGAAGCTATGTACTCTGTTGCTAGTCAATGTCTGCATGAAAAGA AAAATAAGAGACCAGACATTAAGAAGGTTCAACAGCTGCTGCAAGAGATG ACAGCTTCTTAAGCGGCCGC

Amino Acid Sequence

MHHHHHHENLYFQGMSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERD
EGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCP
KERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDR
LCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAI
PQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSVSDTR
FHSFSFYELKNVTNNFDERPISVGGNKMGEGGFGVVYKGYVNNTTVAVKK
LAAMVDITTEELKQQFDQEIKVMAKCQHENLVELLGFSSDGDDLCLVYVY
MPNGSLLDRLSCLDGTPPLSWHMRCKIAQGAANGINFLHENHHIHRDIKS
ANILLDEAFTAKISDFGLARASEKFAQTVMTSRIVGTTAYMAPEALRGEI
TPKSDIYSFGVVLLEIITGLPAVDEHREPQLLLDIKEEIEDEEKTIEDYI
DKKMNDADSTSVEAMYSVASQCLHEKKNKRPDIKKVQQLLQEMTAS*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU65498
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: sub cloned into the Bam-Not backbone of 56013
• Low Temp: No