cDNA Clone Ce_MCM-10

• Synonyms: MCM10
• Plasmid: pGEX6P1 C. elegans MCM10 (G470-S691 end)
• Parent Plasmid: pGEX6P1
• Expressed: GST C. elegans MCM10 (G470-S691 end)
• Genbank: NP_499456.1, NM_067055.4
• Tag: GST
• Species: Caenorhabditis elegans

Sequence of Insert

GGATCCATGGGTTCAAAGCTATGGACAATGTCACTGGCGAATAAGAAAGCTGCTCCGTTAGGTCATAAAGATGCTCAGGAGCATGCCGCTAGGCTCCGCGCAATCGCAATTCTCAAGGCAAAACGAAAAGAAGCCGAAATGGAGAAGCTCACCGGAGGCGGCGGCGGAGTCAAGCGAAAAGCTTCCGGAACACCGACGGCTCCGGAGCCCGAAGCAAAGAAGCCGAGAAGCCAGAAAATGGATGAGATTCGTGCGATGCTCGCCAGAAAGAGCACACATCACAAGGAAGCGGAGAAGGCGGAGCACGACATGCTCCAGCGTCATTTGACAGGAATGGAAGAACGCGAAAAAGTGGAAACATTTACGACGACTTGTGTTGAAGTTAAAAATGTTAAAGTTGTCACGTGTAATCAGTGCAAATACACATCACAATTCGCATCATCAGAATGTATTAAAAAAGAGCATAAACTCATTAAACACACCGCTGACAAGCGATTCTTCAAATGTACTGGATGCAAAAAACGGACTGTTTGTTTTGAAATGATGCCTGTCAAGCATTGTGCGCACTGCAACGAGAACAAATGGGAGCGCGTAGCGATGAAAGACGAACGAAAAGTGGTTCTCGACACGGAAAATCTGCTGCTCCGCGGTGAAGAACGTCCGTTTGTTGGCTCCTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMGSKLWTMSLANKKAAPLGHKDAQEHAARLRAIAILKAKRKEAEMEKLTGGGGGVKRKASGTPTAPEPEAKKPRSQKMDEIRAMLARKSTHHKEAEKAEHDMLQRHLTGMEEREKVETFTTTCVEVKNVKVVTCNQCKYTSQFASSECIKKEHKLIKHTADKRFFKCTGCKKRTVCFEMMPVKHCAHCNENKWERVAMKDERKVVLDTENLLLRGEERPFVGS*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pGEX6P1 C. elegans MCM10 (G470-S691 end)77.38 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU66342
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: This was a direct digest of the PCR product generated by amplifying the fragment from a template plasmid given to us by Karim Labib's lab.
• Low Temp: No