cDNA Clone CAF20 (YOR276W),

• Plasmid: pEX CAF20 (YOR276W) 3C 6His
• Parent Plasmid: pEX c-ter 3C 6His
• Expressed: CAF20 (YOR276W) 3C 6His
• Genbank: ID: 854450
• Tag: 6His
• Species: Yeast

Sequence of Insert

GGATCCATGATCAAGTATACTATCGATGAGCTTTTTCAACTGAAGCCAAGTTTAACTTTGGAAGTTAATTTCGATGCGGTGGAATTTAGAGCCATCATTGAAAAAGTTAAGCAATTGCAACACTTGAAAGAGGAAGAGTTTAACAGTCATCATGTTGGTCATTTCGGTCGTAGAAGATCTTCCCACCATCATGGTAGACCAAAGATTAAGCACAACAAGCCTAAGGTTACAACCGATTCAGATGGTTGGTGCACATTTGAAGCCAAGAAGAAGGGTAGTGGAGAAGATGATGAAGAAGAAACAGAAACCACACCAACTTCTACTGTGCCAGTTGCTACCATTGCCCAAGAAACTTTAAAAGTCAAGCCAAATAACAAAAATATTTCTTCCAACAGACCTGCTGATACCAGAGATATTGTTGCGGACAAGCCAATTCTTGGTTTCAACGCATTTGCTGCTTTGGAAAGTGAAGACGAAGACGACGAAGCAGCGGCCGC

Amino Acid Sequence

MIKYTIDELFQLKPSLTLEVNFDAVEFRAIIEKVKQLQHLKEEEFNSHHVGHFGRRRSSHHHGRPKIKHNKPKVTTDSDGWCTFEAKKKGSGEDDEEETETTPTSTVPVATIAQETLKVKPNNKNISSNRPADTRDIVADKPILGFNAFAALESEDEDDEAAAALEVLFQGPGGGHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pEX CAF20 (YOR276W) 3C 6His143.89 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU66377
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Cloning of CAF20 in phase with a 6xHistidine tag in a pET vector for protein production/purification in bacteria --> Direct digest of PCR product
• Low Temp: No