cDNA Clone BUR6 (YER159C)

• Plasmid: pEX BUR6 (YER159C) 3C 6His
• Parent Plasmid: pEX c-ter 3C 6His
• Expressed: BUR6 (YER159C) 3C 6His
• Genbank: BUR6 (YER159C), ID: 856904
• Tag: 6His
• Species: Yeast

Sequence of Insert

GGATCCATGGCAGATCAAGTACCAGTTACAACACAACTACCACCAATAAAACCTGAACATGAGGTACCACTTGATGCTGGAGGGAGTCCAGTAGGTAACATGGGTACCAACTCGAATAACAACAACGAGCTAGGTGATGTATTCGACAGAATAAAGACACACTTCCCTCCGGCCAAGGTAAAGAAAATAATGCAGACAGACGAGGATATAGGAAAAGTTTCACAAGCCACGCCCGTAATAGCGGGCAGGTCCCTAGAGTTTTTTATAGCGTTATTGGTGAAAAAAAGCGGGGAGATGGCAAGAGGACAAGGAACCAAGAGAATAACCGCCGAAATACTAAAAAAAACAATTTTAAACGACGAAAAATTCGATTTCTTAAGGGAAGGTCTATGCGTAGAAGAAGGCCAAACACAACCGGAGGAAGAGAGTGCC

Amino Acid Sequence

MADQVPVTTQLPPIKPEHEVPLDAGGSPVGNMGTNSNNNNELGDVFDRIKTHFPPAKVKKIMQTDEDIGKVSQATPVIAGRSLEFFIALLVKKSGEMARGQGTKRITAEILKKTILNDEKFDFLREGLCVEEGQTQPEEESAAAALEVLFQGPGGGHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pEX BUR6 (YER159C) 3C 6His143.68 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU66378
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Cloning of yeast BUR6 in phase with a 6xHistidine tag in a pET vector for protein production/purification in bacteria --> direct digest of PCR product
• Low Temp: No