cDNA Clone Laforin

• Synonyms: EPM2, MELF, EPM2A
• Plasmid: pET-Duet1-6His-TEV-laforin, GST-3C-Malin
• Parent Plasmid: pET-Duet1
• Expressed: 6His-TEV-Laforin
• Genbank: NM_005670
• Tag: 6His
• Species: Human

Sequence of Insert

ccATGGGCAGCAGCCATCATCATCATCATCACAGCGAAAACCTGTATTTT
CAGGGCATGCGCTTCCGCTTTGGGGTGGTGGTGCCACCCGCCGTGGCCGG
CGCCCGGCCGGAGCTGCTGGTGGTGGGGTCGCGGCCCGAGCTGGGGCGTT
GGGAGCCGCGCGGTGCCGTCCGCCTGAGGCCGGCCGGCACCGCGGCGGGC
GACGGGGCCCTGGCGCTGCAGGAGCCGGGCCTGTGGCTCGGGGAGGTGGA
GCTGGCGGCCGAGGAGGCGGCGCAGGACGGGGCGGAGCCGGGCCGCGTGG
ACACGTTCTGGTACAAGTTCCTGAAGCGGGAGCCGGGAGGAGAGCTCTCC
TGGGAAGGCAATGGACCTCATCATGACCGTTGCTGTACTTACAATGAAAA
CAACTTGGTGGATGGTGTGTATTGTCTCCCAATAGGACACTGGATTGAGG
CCACTGGACACACCAATGAAATGAAGCACACAACAGACTTCTATTTTAAT
ATTGCAGGCCACCAAGCCATGCATTATTCAAGAATTCTACCAAATATCTG
GCTGGGTAGCTGCCCTCGTCAGGTGGAACATGTAACCATCAAACTGAAGC
ATGAATTGGGGATTACAGCTGTAATGAATTTCCAGACTGAATGGGATATT
GTACAGAATTCCTCAGGCTGTAACCGCTACCCAGAGCCCATGACTCCAGA
CACTATGATTAAACTATATAGGGAAGAAGGCTTGGCCTACATCTGGATGC
CAACACCAGATATGAGCACCGAAGGCCGAGTACAGATGCTGCCCCAGGCG
GTGTGCCTGCTGCATGCGCTGCTGGAGAAGGGACACATCGTGTACGTGCA
CTGCAACGCTGGGGTGGGCCGCTCCACAGCGGCTGTCTGCGGCTGGCTCC
AGTATGTGATGGGCTGGAATCTGAGGAAGGTGCAGTATTTCCTCATGGCC
AAGAGGCCGGCTGTCTACATTGACGAAGAGGCCTTGGCCCGGGCACAAGA
AGATTTTTTCCAGAAATTTGGGAAGGTTCGTTCTTCTGTGTGTAGCCTGT
AGggatcc

Amino Acid Sequence

MGSSHHHHHHSENLYFQGMRFRFGVVVPPAVAGARPELLVVGSRPELGRW
EPRGAVRLRPAGTAAGDGALALQEPGLWLGEVELAAEEAAQDGAEPGRVD
TFWYKFLKREPGGELSWEGNGPHHDRCCTYNENNLVDGVYCLPIGHWIEA
TGHTNEMKHTTDFYFNIAGHQAMHYSRILPNIWLGSCPRQVEHVTIKLKH
ELGITAVMNFQTEWDIVQNSSGCNRYPEPMTPDTMIKLYREEGLAYIWMP
TPDMSTEGRVQMLPQAVCLLHALLEKGHIVYVHCNAGVGRSTAAVCGWLQ
YVMGWNLRKVQYFLMAKRPAVYIDEEALARAQEDFFQKFGKVRSSVCSL*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pET-Duet1-6His-TEV-laforin, GST-3C-Malin90.03 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU65613
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: ORF contains 3 silent changes compared NCBI ref:; Ala53 (GCC > GCG); Gly134 (GGG > GGA); Thr274 (ACC > ACA); ; derives from 65583
• Low Temp: No