cDNA Clone SARS-CoV2 (2019-nCoV) Nsp3

• Plasmid: pSC-B SARS-CoV-2 Nsp3 full length (derived from Addgene clone) (BglII/NotI)
• Parent Plasmid: pSC-B
• Genbank: MN908947.3
• Species: SARS-CoV-2

Sequence of Insert

agatctATGGCCCCTACCAAGGTGACCTTCGGAGACGACACCGTGATCGAGGTGCAGGGTTACAAGAGCGTGAACATCACCTTCGAGCTGGACGAACGCATCGACAAGGTGCTGAACGAAAAGTGCTCCGCTTACACCGTGGAGCTGGGCACCGAGGTGAACGAATTCGCCTGCGTGGTGGCCGACGCTGTGATCAAGACCCTGCAGCCAGTGTCCGAACTGCTGACCCCTCTGGGTATCGACCTGGACGAGTGGTCTATGGCTACCTACTACCTGTTCGACGAGAGCGGCGAATTCAAGCTGGCCTCTCACATGTACTGCAGCTTCTACCCTCCCGACGAAGACGAGGAAGAGGGTGACTGCGAAGAGGAAGAGTTCGAGCCTTCCACTCAGTACGAGTACGGCACCGAAGACGACTACCAGGGCAAGCCTCTGGAATTCGGAGCCACCTCTGCCGCTCTGCAGCCTGAAGAGGAACAGGAGGAAGACTGGCTGGACGACGACTCCCAGCAGACCGTGGGTCAGCAGGACGGCTCTGAAGACAACCAGACCACCACCATCCAGACCATCGTGGAGGTGCAGCCTCAGCTGGAGATGGAACTGACCCCAGTGGTGCAGACCATCGAGGTGAACAGCTTCTCCGGCTACCTGAAGCTGACCGACAACGTGTACATCAAGAACGCTGACATCGTGGAGGAAGCCAAGAAGGTGAAACCAACCGTGGTGGTGAACGCCGCTAACGTGTACCTGAAGCACGGCGGAGGTGTGGCTGGAGCTCTGAACAAGGCTACCAACAACGCCATGCAGGTGGAATCCGACGACTACATCGCTACCAACGGTCCTCTGAAGGTGGGCGGATCCTGCGTGCTGTCTGGCCACAACCTGGCCAAGCACTGCCTGCACGTGGTGGGACCAAACGTGAACAAGGGAGAGGACATCCAGCTGCTGAAGAGCGCCTACGAAAACTTCAACCAGCACGAGGTGCTGCTGGCTCCACTGCTGTCTGCCGGAATCTTCGGTGCTGATCCCATCCACAGCCTGCGCGTGTGCGTGGACACCGTGAGGACCAACGTGTACCTGGCCGTGTTCGACAAGAACCTGTACGACAAGCTGGTGAGCTCCTTCCTGGAGATGAAGTCCGAAAAGCAGGTGGAGCAGAAGATCGCCGAGATCCCCAAGGAGGAAGTGAAGCCTTTCATCACCGAATCTAAGCCCAGCGTGGAGCAGAGAAAGCAGGACGACAAGAAGATCAAGGCCTGCGTGGAGGAAGTGACCACCACCCTGGAGGAAACCAAGTTCCTGACCGAGAACCTGCTGCTGTACATCGACATCAACGGCAACCTGCACCCTGACTCCGCTACCCTGGTGTCTGACATCGACATCACCTTCCTGAAGAAGGACGCCCCCTACATCGTGGGCGACGTGGTGCAGGAAGGAGTGCTGACCGCTGTGGTCATCCCTACCAAGAAGGCCGGTGGCACCACCGAGATGCTGGCCAAGGCTCTGCGTAAGGTGCCAACCGACAACTACATCACCACCTACCCCGGTCAGGGCCTGAACGGTTACACCGTGGAGGAAGCTAAGACCGTGCTGAAGAAGTGCAAGTCTGCCTTCTACATCCTGCCCTCCATCATCTCTAACGAGAAGCAGGAAATCCTGGGCACCGTGAGCTGGAACCTGCGCGAAATGCTGGCCCACGCTGAGGAAACCAGGAAGCTGATGCCCGTGTGCGTGGAGACCAAGGCCATCGTGAGCACCATCCAGAGAAAGTACAAGGGCATCAAGATCCAGGAGGGTGTGGTGGACTACGGCGCTCGTTTCTACTTCTACACCTCCAAGACCACCGTGGCCTCTCTGATCAACACCCTGAACGACCTGAACGAGACCCTGGTGACCATGCCACTGGGATACGTGACCCACGGTCTGAACCTGGAGGAAGCCGCTAGATACATGCGTTCCCTGAAGGTGCCTGCTACCGTGAGCGTGAGCAGCCCTGACGCTGTGACCGCCTACAACGGATACCTGACCTCCTCTAGCAAGACCCCTGAGGAACACTTCATCGAAACCATCAGCCTGGCCGGTTCCTACAAGGACTGGTCTTACAGCGGACAGTCCACCCAGCTGGGCATCGAGTTCCTGAAGCGCGGAGACAAGAGCGTGTACTACACCTCCAACCCAACCACCTTCCACCTGGACGGTGAAGTGATCACCTTCGACAACCTGAAGACCCTGCTGTCTCTGCGCGAGGTGAGGACCATCAAGGTGTTCACCACCGTGGACAACATCAACCTGCACACCCAGGTGGTGGACATGAGCATGACCTACGGACAGCAGTTCGGTCCTACCTACCTGGACGGAGCTGACGTGACCAAGATCAAGCCACACAACTCCCACGAGGGCAAGACCTTCTACGTGCTGCCTAACGATGACACCCTGAGAGTGGAGGCTTTCGAATACTACCACACCACCGACCCATCCTTCCTGGGCAGGTACATGTCTGCCCTGAACCACACCAAGAAGTGGAAGTACCCCCAGGTGAACGGACTGACCAGCATCAAGTGGGCCGACAACAACTGCTACCTGGCCACCGCTCTGCTGACCCTGCAGCAGATCGAACTGAAGTTCAACCCACCCGCTCTGCAGGACGCTTACTACAGAGCCCGTGCTGGCGAGGCCGCTAACTTCTGCGCTCTGATCCTGGCCTACTGCAACAAGACCGTGGGCGAACTGGGAGACGTGAGAGAGACCATGAGCTACCTGTTCCAGCACGCCAACCTGGACTCCTGCAAGCGTGTGCTGAACGTGGTGTGCAAGACCTGCGGCCAGCAGCAGACCACCCTGAAGGGAGTGGAAGCTGTGATGTACATGGGTACCCTGTCCTACGAGCAGTTCAAGAAGGGCGTGCAGATCCCTTGCACCTGCGGAAAGCAGGCCACCAAGTACCTGGTGCAGCAGGAAAGCCCATTCGTGATGATGTCCGCTCCTCCAGCCCAGTACGAACTGAAGCACGGCACCTTCACCTGCGCTTCCGAGTACACCGGCAACTACCAGTGCGGACACTACAAGCACATCACCTCTAAGGAGACCCTGTACTGCATCGACGGAGCCCTGCTGACCAAGTCCTCTGAATACAAGGGTCCCATCACCGACGTGTTCTACAAGGAGAACAGCTACACCACCACCATCAAGCCCGTGACCTACAAGCTGGACGGCGTGGTGTGCACCGAAATCGACCCTAAGCTGGACAACTACTACAAGAAGGACAACTCTTACTTCACCGAGCAGCCTATCGACCTGGTGCCAAACCAGCCCTACCCTAACGCCAGCTTCGACAACTTCAAGTTCGTGTGCGACAACATCAAGTTCGCTGACGACCTGAACCAGCTGACCGGATACAAGAAGCCTGCCTCTAGAGAGCTGAAGGTGACCTTCTTCCCAGACCTGAACGGCGACGTGGTGGCTATCGACTACAAGCACTACACCCCAAGCTTCAAGAAGGGAGCCAAGCTGCTGCACAAGCCCATCGTGTGGCACGTGAACAACGCTACCAACAAGGCCACCTACAAGCCTAACACCTGGTGCATCCGTTGCCTGTGGTCTACCAAGCCAGTGGAAACCTCCAACTCTTTCGACGTGCTGAAGAGCGAGGACGCTCAGGGCATGGACAACCTGGCCTGCGAAGACCTGAAGCCCGTGAGCGAGGAAGTGGTGGAGAACCCAACCATCCAGAAGGACGTGCTGGAATGCAACGTGAAGACCACCGAGGTGGTGGGAGACATCATCCTGAAGCCAGCTAACAACAGCCTGAAGATCACCGAGGAAGTGGGTCACACCGACCTGATGGCCGCTTACGTGGACAACAGCTCCCTGACCATCAAGAAGCCCAACGAGCTGAGCAGGGTGCTGGGTCTGAAGACCCTGGCTACTCACGGTCTGGCTGCTGTGAACTCTGTGCCATGGGACACCATCGCTAACTACGCCAAGCCTTTCCTGAACAAGGTGGTGTCCACCACCACCAACATCGTGACCAGATGTCTGAACCGCGTGTGCACCAACTACATGCCCTACTTCTTCACCCTGCTGCTGCAGCTGTGCACCTTCACCAGAAGCACCAACTCCCGTATCAAGGCTAGCATGCCTACCACCATCGCCAAGAACACCGTGAAGAGCGTGGGAAAGTTCTGCCTGGAGGCTTCCTTCAACTACCTGAAGTCTCCAAACTTCAGCAAGCTGATCAACATCATCATCTGGTTCCTGCTGCTCTCCGTGTGCCTGGGTTCCCTGATCTACTCTACCGCCGCTCTGGGAGTGCTGATGTCTAACCTGGGTATGCCTAGCTACTGCACTGGTTACAGAGAGGGATACCTGAACAGCACCAACGTGACCATCGCCACCTACTGCACCGGCAGCATCCCATGCTCCGTGTGCCTGTCTGGACTGGACAGCCTGGACACCTACCCCTCCCTGGAAACCATCCAGATCACCATCTCTAGCTTCAAGTGGGACCTGACCGCCTTCGGTCTGGTGGCTGAGTGGTTCCTGGCCTACATCCTGTTCACCAGGTTCTTCTACGTGCTGGGCCTGGCCGCTATCATGCAGCTGTTCTTCTCCTACTTCGCTGTGCACTTCATCAGCAACTCCTGGCTGATGTGGCTGATCATCAACCTGGTGCAGATGGCTCCCATCTCTGCCATGGTGCGCATGTACATCTTCTTCGCCAGCTTCTACTACGTGTGGAAGTCCTACGTGCACGTGGTGGACGGTTGCAACTCCTCTACCTGCATGATGTGCTACAAGCGCAACAGGGCTACCAGGGTGGAGTGCACCACCATCGTGAACGGCGTGCGCAGGTCCTTCTACGTGTACGCCAACGGCGGCAAGGGATTCTGCAAGCTGCACAACTGGAACTGCGTGAACTGCGACACCTTCTGCGCTGGCTCCACCTTCATCTCTGACGAGGTGGCCAGAGACCTGAGCCTGCAGTTCAAGCGTCCAATCAACCCCACCGACCAGAGCTCCTACATCGTGGACTCTGTGACCGTGAAGAACGGTAGCATCCACCTGTACTTCGACAAGGCTGGCCAGAAGACCTACGAGCGCCACTCTCTGAGCCACTTCGTGAACCTGGACAACCTGAGGGCCAACAACACCAAGGGCAGCCTGCCTATCAACGTGATCGTGTTCGACGGAAAGTCCAAGTGCGAGGAATCTAGCGCCAAGTCCGCCAGCGTGTACTACAGCCAGCTGATGTGCCAGCCAATCCTGCTGCTGGACCAGGCTCTGGTGTCTGACGTGGGAGACAGCGCCGAAGTGGCCGTGAAGATGTTCGACGCCTACGTGAACACCTTCTCCTCTACCTTCAACGTGCCTATGGAGAAGCTGAAGACCCTGGTGGCTACTGCTGAGGCTGAACTGGCTAAGAACGTGTCCCTGGACAACGTGCTGAGCACCTTCATCTCCGCCGCTAGGCAGGGTTTCGTGGACTCTGACGTGGAAACCAAAGACGTGGTGGAGTGCCTGAAGCTGTCTCACCAGAGCGACATCGAAGTGACCGGCGACTCCTGCAACAACTACATGCTGACCTACAACAAGGTGGAGAACATGACCCCAAGAGACCTGGGAGCTTGCATCGACTGCTCCGCCCGTCACATCAACGCTCAGGTGGCCAAGTCTCACAACATCGCCCTGATCTGGAACGTGAAGGACTTCATGAGCCTGTCCGAGCAGCTGCGCAAGCAGATCAGGAGCGCCGCTAAGAAGAACAACCTGCCCTTCAAGCTGACCTGCGCTACCACCAGACAGGTGGTGAACGTGGTGACCACCAAGATCGCCCTGAAGGGCGGATAAgcggccgc

Amino Acid Sequence

MAPTKVTFGDDTVIEVQGYKSVNITFELDERIDKVLNEKCSAYTVELGTEVNEFACVVADAVIKTLQPVSELLTPLGIDLDEWSMATYYLFDESGEFKLA
SHMYCSFYPPDEDEEEGDCEEEEFEPSTQYEYGTEDDYQGKPLEFGATSAALQPEEEQEEDWLDDDSQQTVGQQDGSEDNQTTTIQTIVEVQPQLEMELT
PVVQTIEVNSFSGYLKLTDNVYIKNADIVEEAKKVKPTVVVNAANVYLKHGGGVAGALNKATNNAMQVESDDYIATNGPLKVGGSCVLSGHNLAKHCLHV
VGPNVNKGEDIQLLKSAYENFNQHEVLLAPLLSAGIFGADPIHSLRVCVDTVRTNVYLAVFDKNLYDKLVSSFLEMKSEKQVEQKIAEIPKEEVKPFITE
SKPSVEQRKQDDKKIKACVEEVTTTLEETKFLTENLLLYIDINGNLHPDSATLVSDIDITFLKKDAPYIVGDVVQEGVLTAVVIPTKKAGGTTEMLAKAL
RKVPTDNYITTYPGQGLNGYTVEEAKTVLKKCKSAFYILPSIISNEKQEILGTVSWNLREMLAHAEETRKLMPVCVETKAIVSTIQRKYKGIKIQEGVVD
YGARFYFYTSKTTVASLINTLNDLNETLVTMPLGYVTHGLNLEEAARYMRSLKVPATVSVSSPDAVTAYNGYLTSSSKTPEEHFIETISLAGSYKDWSYS
GQSTQLGIEFLKRGDKSVYYTSNPTTFHLDGEVITFDNLKTLLSLREVRTIKVFTTVDNINLHTQVVDMSMTYGQQFGPTYLDGADVTKIKPHNSHEGKT
FYVLPNDDTLRVEAFEYYHTTDPSFLGRYMSALNHTKKWKYPQVNGLTSIKWADNNCYLATALLTLQQIELKFNPPALQDAYYRARAGEAANFCALILAY
CNKTVGELGDVRETMSYLFQHANLDSCKRVLNVVCKTCGQQQTTLKGVEAVMYMGTLSYEQFKKGVQIPCTCGKQATKYLVQQESPFVMMSAPPAQYELK
HGTFTCASEYTGNYQCGHYKHITSKETLYCIDGALLTKSSEYKGPITDVFYKENSYTTTIKPVTYKLDGVVCTEIDPKLDNYYKKDNSYFTEQPIDLVPN
QPYPNASFDNFKFVCDNIKFADDLNQLTGYKKPASRELKVTFFPDLNGDVVAIDYKHYTPSFKKGAKLLHKPIVWHVNNATNKATYKPNTWCIRCLWSTK
PVETSNSFDVLKSEDAQGMDNLACEDLKPVSEEVVENPTIQKDVLECNVKTTEVVGDIILKPANNSLKITEEVGHTDLMAAYVDNSSLTIKKPNELSRVL
GLKTLATHGLAAVNSVPWDTIANYAKPFLNKVVSTTTNIVTRCLNRVCTNYMPYFFTLLLQLCTFTRSTNSRIKASMPTTIAKNTVKSVGKFCLEASFNY
LKSPNFSKLINIIIWFLLLSVCLGSLIYSTAALGVLMSNLGMPSYCTGYREGYLNSTNVTIATYCTGSIPCSVCLSGLDSLDTYPSLETIQITISSFKWD
LTAFGLVAEWFLAYILFTRFFYVLGLAAIMQLFFSYFAVHFISNSWLMWLIINLVQMAPISAMVRMYIFFASFYYVWKSYVHVVDGCNSSTCMMCYKRNR
ATRVECTTIVNGVRRSFYVYANGGKGFCKLHNWNCVNCDTFCAGSTFISDEVARDLSLQFKRPINPTDQSSYIVDSVTVKNGSIHLYFDKAGQKTYERHS
LSHFVNLDNLRANNTKGSLPINVIVFDGKSKCEESSAKSASVYYSQLMCQPILLLDQALVSDVGDSAEVAVKMFDAYVNTFSSTFNVPMEKLKTLVATAE
AELAKNVSLDNVLSTFISAARQGFVDSDVETKDVVECLKLSHQSDIEVTGDSCNNYMLTYNKVENMTPRDLGACIDCSARHINAQVAKSHNIALIWNVKD
FMSLSEQLRKQIRSAAKKNNLPFKLTCATTRQVVNVVTTKIALKGG*

• Vector Type: Other
• Antibiotic: Amp; Kan
• Full Snapgene Map: pSC-B SARS-CoV-2 Nsp3 full length (derived from Addgene clone) (BglII/NotI)39.85 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU67792
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Codon optimised to reduce gc-content
• Low Temp: No