cDNA Clone Ube2o (mouse)

• Synonyms: E2-230K; mKIAA1734; 9630022H21; B230113M03Rik
• Plasmid: pCAGGS puro HA mouse Ube2o S406A
• Parent Plasmid: pCAGGS puro HA
• Expressed: HA mouse Ube2o S406A
• Genbank: NM_173755.3
• Tag: HA
• Species: Mouse

Sequence of Insert

AAGCTTATGGCGGATCCCGCAGCGCCCGCACCCGCCCAGGCCCAGGCCGCCGCCGCCCCGACCCCGGCCGCAGCCCCCGCCGCCGCCGCCCCGCCGCCCGCGCCCGCTACGGACTCGGCCTCAGGGCCGTCCTCCGACTCCGGCCCCGAAGCAGGCTCACAGCGCCTGCTGTTCTCCCACGACCTGGTGTCCGGCCGTTACCGCGGCTCGGTGCACTTCGGGCTGGTACGCCTCATCCACGGCGAGGACTCGGACTCGGAGGGCGACGATGACGGCCGCGGCAGCTCGGGCTGCTCCGAGGCCGGGGGCGCGGGCCACGAGGAGGGCCGGGCCAGCCCTCTGCGGCGCGGCTACGTGCGCGTCCAGTGGTACCCGGAGGGCGTCAAGCAGCATGTGAAGGAGACCAAGCTGAAACTGGAGGACCGCTCTGTGGTGCCCCGGGATGTGGTTCGACACATGCGCTCAACTGACAGCCAGTGTGGCACGGTGATAGATGTCAACATTGACTGTGCCGTCAAGCTCATTGGCACCAACTGCATCATCTACCCTGTCAACAGCAAGGACCTCCAGCACATCTGGCCCTTCATGTATGGAGACTATATCGCCTATGACTGCTGGCTGGGGAAGGTCTATGACTTGAAGAACCAGATCATCCTCAAGTTGTCTAATGGTGCCAGGTGCTCCATGAACACAGAAGATGGTGCCAAACTCTACGATGTCTGCCCTCACGTCAGCGACTCGGGTCTCTTCTTTGATGACTCCTATGGCTTCTACCCAGGCCAAGTCCTTATTGGCCCAGCCAAGATCTTCTCCAGTGTCCAGTGGCTCTCGGGGGTCAAGCCTGTACTCAGCACCAAGAGCAAGTTCCGTGTGGTGGTGGAGGAGGTGCAGGTTGTAGAACTGAAAGTCACGTGGATTACCAAGAGCTTCTGCCCCGGCGGCACGGACAGCGTTAGCCCCCCACCTTCCATCATCACTCAGGAAAACCTAGGCAGGGTGAAGCGTCTCGGATGCTTTGACCATGCTCAGCGGCAGCTTGGGGAACGCTGTCTGTATGTCTTCCCAGCCAAGGTAGAGCCGGCCAAGATTGCCTGGGAATGTCCAGAAAAAAACTGCGCCCAGGGGGAGGGCTCTATGGCCAAGAAGGTGAAGCGTCTGTTGAAGAAGCAAGTTGTGAGAATCATGTCCTGCACCCCAGATACCCAGTGTCCTAGGGACCATGCTATGGAAGACCCAGACAAGAAAGGGGAAGCCAGAGCAGGGAGTGAAATAGGGTCTGCTAGCCCGGAGGAGCAACCTGATGGATCAGCCAGCCCCGTGGAGATGCAGGATGAGGGCTCGGAGGAGCTTCAGGAGACATGCGAGCCACTGCCCCCCTTCCTCTTGAAGGAGGGTGGAGATGACGGGCTGCACTCGGCAGAGCAGGACGCAGATGATGAGGCGGCTGACGATACAGATGACACCAGCTCTGTGACCTCCTCCGCCAGCTCTACTACCTCCTCCCAGAGTGGCAGTGGCACTGGTCGTAAAAAGAGCATTCCTTTGTCCATCAAAAACTTGAAGCGAAAACACAAGAGGAAGAAGAATAAGGTCACCCGTGACTTCAAGCCTGGAGACAGGGTGGCAGTGGAGGTTGTGACCACGATGACCTCAGCTGATGTGATGTGGCAAGATGGCTCTGTGGAATGCAACATCCGCTCCAATGACCTTTTCCCTGTGCACCACCTGGACAACAATGAGTTCTGCCCTGGGGACTTCGTGGTGGACAAGCGAGTCCAGAGCTGCCCAGACCCTGCTGTCTATGGTGTGGTGCAGTCTGGGGATCATGTTGGCCGTACCTGCATGGTGAAGTGGTTCAAGCTGCGACCAAGTGGGGATGACGTGGAGCTCATTGGAGAAGAGGAGGACGTGAGCGTCTATGACATTGCTGACCACCCTGACTTTCGCTTCCGCACAACTGACATTGTCATCCGCATTGGTAACACGGAGGATGGGGCTCTACCTAAGGAGGATGAGCCCTCAGTGGGCCAAGTGGCCCGAGTGGATGTCAGCAGCAAAGTGGAAGTGGTGTGGGCTGACAACTCGAAAACCATCATCCTGCCACAGCACTTATACAACATCGAGTCTGAGATTGAGGAGTCTGACTACGACTCCGTGGAAGGCAGCTCCAGTGGGGCCTCCTCGGATGAGTGGGAGGACGACAGCGACAGCTGGGAGACAGACAACGGGCTCGTGGACGATGAGCACCCCAAGATAGAGGAGCTGGCCGCCATCCTGCCTGCCGAGCAGCCCACTGCCCCCGAGGAGGACAAGGGCGTGGTCATCAGTGAGGAGGCGGCCACGGCTGCCATCCAGGGCGCCGTGGCCATGGCTGCCCCCGTGGCAGGGCTGATGGAGAAAGCTGGGAAGGACGGGCCTCCCAAGAGTTTCCGGGAGCTGAAGGAGGCCATCAAGATCCTGGAGAGCCTCAAGAACATGACTGTGGAGCAGCTGCTCACAGGCTCGCCCACCTCGCCCACCGTGGAGCCTGAGAAGCCAACCCGAGAGAAGAAGTTTTTGGATGATATCAAGAAGCTACAGGAGAACCTCAAGAAGACCCTGGACAATGTGGCCATTGCTGAGGAGGAGAAGATGGAGGCAGTACCCGACACAGAGCGCAAGGAGGAAAAGCCTGAGGTGCAGTCCCCAGTGAAAGCCGAGTGGCCCAGTGAGACACCTGTGCTCTGTCAGCAGTGCGGTGGCAGGCCTGGCGTCACCTTCACCAGCGCCAAGGGCGAGGTCTTCTCTGTGCTGGAGTTTGCACCCTCAAACCACTCTTTCAAGAAGATTGAGTTCCAGCCCCCGGAAGCCAAGAAGTTCTTCAGCACAGTGCGGAAGGAGATGGCTCTGCTGGCCACCTCGCTGCCCGATGGCATCATGGTCAAGACTTTTGAGGACAGAATGGACCTCTTCTCTGCACTGATCAAGGGCCCCACTCGGACTCCCTATGAAGACGGCCTCTACCTATTTGACATCCAGCTTCCCAACATCTACCCTGCGGTGCCCCCCCACTTTTGCTACCTCTCCCAGTGCAGTGGCCGCCTGAACCCTAACCTGTATGACAATGGGAAAGTGTGTGTCAGCCTCCTAGGTACCTGGATTGGAAAGGGGACAGAGAGGTGGACAAGCAAGTCTAGCCTTCTCCAGGTGCTCATCTCCATCCAAGGTCTGATCCTGGTGAATGAACCATACTACAATGAAGCTGGCTTTGACAGTGACCGGGGCCTGCAGGAAGGCTATGAGAACAGTCGCTGCTACAACGAGATGGCATTAATCCGTGTGGTGCAGTCCATGACCCAGTTGGTGCGACGACCTCCTGAGGTCTTCGAACAGGAGATTCGGCAGCACTTCAGTGTGGGTGGCTGGCGGCTGGTGAACCGAATTGAGTCCTGGCTGGAAACCCATGCCATGCAGGAGAGGGCCCAGGTGATGCCCAATGGGGCCCCTAAAGACAGCAGCTCTCTGGAGCCCATGGCTGCAGCTGAACTCTCAGACTCCGGCCGTGAAGAGCCTGAAGATGTTGGGATGGCCCCTGGGGAGGCGTCCCAGGGCTCAGACTCAGAGGGTGGAGCCCAGGGCCCTGCCTCAGCCAGCAGAGACCACACAGAGCAGACTGAGACAGCCCCTGATGCATCCGCACCTCCCAGCGTTAGACCAAAGAGGAGGAGGAAGAGCTACCGCAGCTTCCTGCCTGAGAAGAGCGGTTACCCTGACATCGGCTTCCCCCTCTTCCCACTCTCCAAGGGTTTCATCAAGAGCATCCGGGGGGTCCTGACACAGTTCCGTGCTGCCCTGCTGGAGGCGGGCATGCCCGAAAGCACAGAGGACAAGTAGGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSGKLMADPAAPAPAQAQAAAAPTPAAAPAAAAPPPAPATDSASGPSSDSGPEAGSQRLLFSHDLVSGRYRGSVHFGLVRLIHGEDSDSE
GDDDGRGSSGCSEAGGAGHEEGRASPLRRGYVRVQWYPEGVKQHVKETKLKLEDRSVVPRDVVRHMRSTDSQCGTVIDVNIDCAVKLIGTNCIIYPVNSK
DLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGVKPVLSTKS
KFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSIITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVEPAKIAWECPEKNCAQGEGSMAKKVKRLL
KKQVVRIMSCTPDTQCPRDHAMEDPDKKGEARAGSEIGSASPEEQPDGSASPVEMQDEGSEELQETCEPLPPFLLKEGGDDGLHSAEQDADDEAADDTDD
TSSVTSSASSTTSSQSGSGTGRKKSIPLSIKNLKRKHKRKKNKVTRDFKPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDNNEFCPGDFV
VDKRVQSCPDPAVYGVVQSGDHVGRTCMVKWFKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGALPKEDEPSVGQVARVDVSSKV
EVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSSSGASSDEWEDDSDSWETDNGLVDDEHPKIEELAAILPAEQPTAPEEDKGVVISEEAATAAIQGA
VAMAAPVAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDNVAIAEEEKMEAVPDTERK
EEKPEVQSPVKAEWPSETPVLCQQCGGRPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMDLFSAL
IKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDR
GLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSVGGWRLVNRIESWLETHAMQERAQVMPNGAPKDSSSLEPMAAAELSDSGREEPEDV
GMAPGEASQGSDSEGGAQGPASASRDHTEQTETAPDASAPPSVRPKRRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQFRAALLEAGMPEST
EDK*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pCAGGS puro HA mouse Ube2o S406A1.06 MBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU61854
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Compared to reference sequence there are silent changes at I173, S252, S275, A418, G444, P512, N566, P666, Q675, T738, L757, D852, E887 and P1028. There is also a SNP at L1160P.
• Low Temp: No