Full Name
IKK epsilon (701 - 716)
Long Name Textual
inhibitory kB kinase epsilon
Immuno Sequence
NRIIERLNRVPAPPDV [residues 701 - 716 of human]
Mono/Poly clonal
Polyclonal
Gel Image
![25 ug of cell lysates from various breast cancer cell lines and HEK293 cells transfected with either empty vector (UTR) or FLAG tagged IKK epsilon were separated on an 8 % SDS-Page gel and transferred to PDVF membrane. The membrane was immunoblotted with anti-IKK epsilon (701 – 716) at 1 ug/ml. Binding of the primary antibody was detected using rabbit peroxidase conjugated anti-sheep IgG antibody (1 in 10, 000 dilution, Pierce) followed by enhanced chemiluminescence (ECL, Amersham). IKK epsilon](/sites/default/files/styles/gel_image/public/gel_images/IKK-epsilon_701-716_S255C_IB.jpg?itok=ekC-s0tP)
25 ug of cell lysates from various breast cancer cell lines and HEK293 cells transfected with either empty vector (UTR) or FLAG tagged IKK epsilon were separated on an 8 % SDS-Page gel and transferred to PDVF membrane. The membrane was immunoblotted with anti-IKK epsilon (701 – 716) at 1 ug/ml. Binding of the primary antibody was detected using rabbit peroxidase conjugated anti-sheep IgG antibody (1 in 10, 000 dilution, Pierce) followed by enhanced chemiluminescence (ECL, Amersham).
![25 ug of HEK293 cells transfected with either empty vector (control), FLAG tagged TBK1 or FLAG tagged IKK epsilon were separated on an 8 % SDS-Page gel and transferred to PDVF membrane. The membrane was immunoblotted with anti-IKK epsilon (701 – 716) at 1 ug/ml (upper panel) or anti-FLAG M2 [Sigma] (lower panel). Binding of the primary antibody was detected using rabbit peroxidase conjugated anti- sheep IgG antibody (1 in 10, 000 dilution, Pierce) followed by enhanced chemiluminescence (ECL, Amersham).](/sites/default/files/styles/gel_image/public/gel_images/IKK-epsilon_701-716_S255C_IP.jpg?itok=R9z27ikk)
25 ug of HEK293 cells transfected with either empty vector (control), FLAG tagged TBK1 or FLAG tagged IKK epsilon were separated on an 8 % SDS-Page gel and transferred to PDVF membrane. The membrane was immunoblotted with anti-IKK epsilon (701 – 716) at 1 ug/ml (upper panel) or anti-FLAG M2 [Sigma] (lower panel). Binding of the primary antibody was detected using rabbit peroxidase conjugated anti- sheep IgG antibody (1 in 10, 000 dilution, Pierce) followed by enhanced chemiluminescence (ECL, Amersham).
![1 mg of protein extract from RAW264.7 macrophages was incubated with pre-immune IgG (Ig) or either the first, second or third bleeds of affinity purified anti-IKK epsilon (701–716)at2or5ug. Thesampleswereincubatedfor1hrat4degConanendover end roller. 10 ul of Protein G Sepharose was added and incubated for a further 15 mins at 4 deg C. The beads were collected by centrifugation and washed three times in lysis buffer. The immunoprecipitates were separated on an 8 % SDS-Page gel, along with 20 ug of total protein extract (TL), and transferred to PDVF membrane. The membrane was immunoblotted with anti-IKK epsilon (Sigma) followed by HRP-conjugated anti-mouse antibodies with detection by enhanced chemiluminescence (ECL, Amersham).](/sites/default/files/styles/gel_image/public/gel_images/IKK-epsilon_701-716_S255C_IP_2.jpg?itok=CwwpLk_t)
1 mg of protein extract from RAW264.7 macrophages was incubated with pre-immune IgG (Ig) or either the first, second or third bleeds of affinity purified anti-IKK epsilon (701–716)at2or5ug. Thesampleswereincubatedfor1hrat4degConanendover end roller. 10 ul of Protein G Sepharose was added and incubated for a further 15 mins at 4 deg C. The beads were collected by centrifugation and washed three times in lysis buffer. The immunoprecipitates were separated on an 8 % SDS-Page gel, along with 20 ug of total protein extract (TL), and transferred to PDVF membrane. The membrane was immunoblotted with anti-IKK epsilon (Sigma) followed by HRP-conjugated anti-mouse antibodies with detection by enhanced chemiluminescence (ECL, Amersham).
PDF Datasheet
IKK epsilon373.73 KB
Sheep No
S255C
Unit Source
Aliquot
0.1mg
Price
£125.00