cDNA Clone Ubiquitin

• Plasmid: pSC-B-Ubiquitin c220a
• Parent Plasmid: pSC-B
• Genbank: P62988*
• Species: Human

Sequence of Insert

CATATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGA
AGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACA
AGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAG
CTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCAC
CCTGCACCTGGTCCTCCGTCTCCGAGGTGGGTGATAAGGATCC

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU20028
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Source plasmid for Ubiquitin (Arg73 codon AGA mutated to more common CGA); Differs from reference sequence in having silent mutation at Arg73 (a220c substitution conferred by reverse primer 54389)