cDNA Clone parkin (Drosophila)

• Synonyms: Dmel_CG10523, CG10523, Dmel\CG10523, SD01679, dpk, CG10523-PB, CG10523-PC, D-parkin, dParkin, park-PB, park-PC, PARK2
• Plasmid: pSC-B-parkin-d S94E Bam Xho (insect kozak bam frame2)
• Parent Plasmid: pSC-B
• Genbank: NM_168884.1
• Species: Drosophila Melanogaster

Sequence of Insert

GGATCCCAAAATGAGTTTTATTTTTAAATTTATTGCCACTTTTGTACGCAAAATGCTGGAGCTGTTGCAATTTGGAGGGAAAACCCTTACGCACACGCTGAGTATTTATGTAAAAACGAACACGGGCAAAACGCTGACCGTTAATCTGGAGCCCCAGTGGGATATTAAAAACGTAAAGGAACTGGTGGCACCACAATTGGGACTTCAGCCGGATGATCTAAAGATCATATTTGCCGGTAAGGAACTAAGCGATGCCACGACAATAGAGCAATGTGACTTGGGTCAGCAAGAAGTTTTGCATGCCATTCGTTTGCGACCGCCCGTGCAGCGCCAGAAAATCCAGTCGGCCACTTTGGAGGAGGAGGAACCTTCGCTTAGCGATGAAGCCTCCAAGCCTCTAAATGAAACTCTGTTGGACTTGCAGCTGGAAAGCGAGGAGCGTCTGAATATAACCGATGAAGAAAGAGTCCGTGCCAAAGCGCATTTCTTTGTACATTGCAGCCAATGCGATAAGCTGTGTAATGGCAAACTTCGGGTCCGCTGCGCTTTATGCAAGGGCGGCGCCTTTACTGTCCATCGTGATCCGGAGTGTTGGGATGATGTCTTGAAGTCGCGTAGAATTCCCGGTCATTGTGAAAGTCTGGAGGTGGCCTGCGTGGACAACGCGGCTGGCGATCCGCCCTTCGCTGAATTCTTCTTCAAGTGTGCTGAGCATGTCTCCGGCGGGGAGAAGGATTTTGCGGCTCCATTGAATCTAATCAAGAATAACATCAAGAATGTTCCTTGCCTGGCCTGCACGGATGTGAGTGATACCGTGTTGGTTTTCCCCTGCGCATCGCAGCACGTCACCTGTATCGACTGCTTCCGCCATTATTGCCGTTCCCGTTTGGGCGAGCGTCAGTTTATGCCGCATCCGGACTTCGGCTACACCTTGCCCTGTCCCGCAGGCTGCGAGCACTCGTTCATCGAGGAGATTCATCACTTCAAGCTGTTGACACGCGAGGAGTACGATCGGTACCAGAGATTCGCCACCGAGGAGTATGTCCTACAGGCAGGTGGAGTACTCTGCCCCCAGCCAGGATGCGGCATGGGCCTTTTGGTGGAGCCCGATTGTCGCAAGGTGACATGCCAGAACGGCTGTGGATACGTGTTCTGCCGCAATTGTCTGCAGGGCTACCATATCGGGGAGTGTCTGCCCGAGGGGACGGGCGCCAGTGCCACAAACTCCTGCGAGTACACCGTGGACCCAAATCGAGCTGCCGAGGCGCGATGGGATGAGGCCAGCAATGTCACCATCAAAGTCAGCACCAAGCCCTGTCCAAAATGCAGAACCCCAACGGAGCGAGATGGCGGTTGCATGCACATGGTCTGCACACGCGCTGGCTGCGGATTCGAGTGGTGCTGGGTCTGCCAGACTGAGTGGACACGCGACTGCATGGGAGCCCACTGGTTCGGCTAACTCGAG

Amino Acid Sequence

MSFIFKFIATFVRKMLELLQFGGKTLTHTLSIYVKTNTGKTLTVNLEPQWDIKNVKELVAPQLGLQPDDLKIIFAGKELSDATTIEQCDLGQQEVLHAIRLRPPVQRQKIQSATLEEEEPSLSDEASKPLNETLLDLQLESEERLNITDEERVRAKAHFFVHCSQCDKLCNGKLRVRCALCKGGAFTVHRDPECWDDVLKSRRIPGHCESLEVACVDNAAGDPPFAEFFFKCAEHVSGGEKDFAAPLNLIKNNIKNVPCLACTDVSDTVLVFPCASQHVTCIDCFRHYCRSRLGERQFMPHPDFGYTLPCPAGCEHSFIEEIHHFKLLTREEYDRYQRFATEEYVLQAGGVLCPQPGCGMGLLVEPDCRKVTCQNGCGYVFCRNCLQGYHIGECLPEGTGASATNSCEYTVDPNRAAEARWDEASNVTIKVSTKPCPKCRTPTERDGGCMHMVCTRAGCGFEWCWVCQTEWTRDCMGAHWFG*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU37920
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: S94E mutation. BamHI not in frame one, needs rePCR. Silent A>C 1299 bp and C>A 1326 bp.