cDNA Clone IRAK1

• Plasmid: pcDNA5 FRT/TO Puro IRAK1 K134R K180R
• Parent Plasmid: pcDNA5 FRT/TO Puro
• Expressed: IRAK1 K134R K180R
• Species: Human

Sequence of Insert

GCGGCCGCGATGGCCGGGGGGCCGGGCCCGGGGGAGCCCGCAGCCCCCGG CGCCCAGCACTTCTTGTACGAGGTGCCGCCCTGGGTCATGTGCCGCTTCT ACAAAGTGATGGACGCCCTGGAGCCCGCCGACTGGTGCCAGTTCGCCGCC CTGATCGTGCGCGACCAGACCGAGCTGCGGCTGTGCGAGCGCTCCGGGCA GCGCACGGCCAGCGTCCTGTGGCCCTGGATCAACCGCAACGCCCGTGTGG CCGACCTCGTGCACATCCTCACGCACCTGCAGCTGCTCCGTGCGCGGGAC ATCATCACAGCCTGGCACCCTCCCGCCCCGCTTCCGTCCCCAGGCACCAC TGCCCCGAGGCCCAGCAGCATCCCTGCACCCGCCGAGGCCGAGGCCTGGA GCCCCCGGAGGTTGCCATCCTCAGCCTCCACCTTCCTCTCCCCAGCTTTT CCAGGCTCCCAGACCCATTCAGGGCCTGAGCTCGGCCTGGTCCCAAGCCC TGCTTCCCTGTGGCCTCCACCGCCATCTCCAGCCCCTTCTTCTACCAGGC CAGGCCCAGAGAGCTCAGTGTCCCTCCTGCAGGGAGCCCGCCCCTCTCCG TTTTGCTGGCCCCTCTGTGAGATTTCCCGGGGCACCCACAACTTCTCGGA GGAGCTCAAGATCGGGGAGGGTGGCTTTGGGTGCGTGTACCGGGCGGTGA TGAGGAACACGGTGTATGCTGTGAAGAGGCTGAAGGAGAACGCTGACCTG GAGTGGACTGCAGTGAAGCAGAGCTTCCTGACCGAGGTGGAGCAGCTGTC CAGGTTTCGTCACCCAAACATTGTGGACTTTGCTGGCTACTGTGCTCAGA ACGGCTTCTACTGCCTGGTGTACGGCTTCCTGCCCAACGGCTCCCTGGAG GACCGTCTCCACTGCCAGACCCAGGCCTGCCCACCTCTCTCCTGGCCTCA GCGACTGGACATCCTTCTGGGTACAGCCCGGGCAATTCAGTTTCTACATC AGGACAGCCCCAGCCTCATCCATGGAGACATCAAGAGTTCCAACGTCCTT CTGGATGAGAGGCTGACACCCAAGCTGGGAGACTTTGGCCTGGCCCGGTT CAGCCGCTTTGCCGGGTCCAGCCCCAGCCAGAGCAGCATGGTGGCCCGGA CACAGACAGTGCGGGGCACCCTGGCCTACCTGCCCGAGGAGTACATCAAG ACGGGAAGGCTGGCTGTGGACACGGACACCTTCAGCTTTGGGGTGGTAGT GCTAGAGACCTTGGCTGGTCAGAGGGCTGTGAAGACGCACGGTGCCAGGA CCAAGTATCTGAAAGACCTGGTGGAAGAGGAGGCTGAGGAGGCTGGAGTG GCTTTGAGAAGCACCCAGAGCACACTGCAAGCAGGTCTGGCTGCAGATGC CTGGGCTGCTCCCATCGCCATGCAGATCTACAAGAAGCACCTGGACCCCA GGCCCGGGCCCTGCCCACCTGAGCTGGGCCTGGGCCTGGGCCAGCTGGCC TGCTGCTGCCTGCACCGCCGGGCCAAAAGGAGGCCTCCTATGACCCAGGT GTACGAGAGGCTAGAGAAGCTGCAGGCAGTGGTGGCGGGGGTGCCCGGGC ATTTGGAGGCCGCCAGCTGCATCCCCCCTTCCCCGCAGGAGAACTCCTAC GTGTCCAGCACTGGCAGAGCCCACAGTGGGGCTGCTCCATGGCAGCCCCT GGCAGCGCCATCAGGAGCCAGTGCCCAGGCAGCAGAGCAGCTGCAGAGAG GCCCCAACCAGCCCGTGGAGAGTGACGAGAGCCTAGGCGGCCTCTCTGCT GCCCTGCGCTCCTGGCACTTGACTCCAAGCTGCCCTCTGGACCCAGCACC CCTCAGGGAGGCCGGCTGTCCTCAGGGGGACACGGCAGGAGAATCGAGCT GGGGGAGTGGCCCAGGATCCCGGCCCACAGCCGTGGAAGGACTGGCCCTT GGCAGCTCTGCATCATCGTCGTCAGAGCCACCGCAGATTATCATCAACCC TGCCCGACAGAAGATGGTCCAGAAGCTGGCCCTGTACGAGGATGGGGCCC TGGACAGCCTGCAGCTGCTGTCGTCCAGCTCCCTCCCAGGCTTGGGCCTG GAACAGGACAGGCAGGGGCCCGAAGAAAGTGATGAATTTCAGAGCTGAGC GGCCGC

Amino Acid Sequence

MAGGPGPGEPAAPGAQHFLYEVPPWVMCRFYKVMDALEPADWCQFAALIVRDQTELRLCERSGQRTASVLWPWINRNARVADLVHILTHLQLLRARDIIT
AWHPPAPLPSPGTTAPRPSSIPAPAEAEAWSPRRLPSSASTFLSPAFPGSQTHSGPELGLVPSPASLWPPPPSPAPSSTRPGPESSVSLLQGARPSPFCW
PLCEISRGTHNFSEELKIGEGGFGCVYRAVMRNTVYAVKRLKENADLEWTAVKQSFLTEVEQLSRFRHPNIVDFAGYCAQNGFYCLVYGFLPNGSLEDRL
HCQTQACPPLSWPQRLDILLGTARAIQFLHQDSPSLIHGDIKSSNVLLDERLTPKLGDFGLARFSRFAGSSPSQSSMVARTQTVRGTLAYLPEEYIKTGR
LAVDTDTFSFGVVVLETLAGQRAVKTHGARTKYLKDLVEEEAEEAGVALRSTQSTLQAGLAADAWAAPIAMQIYKKHLDPRPGPCPPELGLGLGQLACCC
LHRRAKRRPPMTQVYERLEKLQAVVAGVPGHLEAASCIPPSPQENSYVSSTGRAHSGAAPWQPLAAPSGASAQAAEQLQRGPNQPVESDESLGGLSAALR
SWHLTPSCPLDPAPLREAGCPQGDTAGESSWGSGPGSRPTAVEGLALGSSASSSSEPPQIIINPARQKMVQKLALYEDGALDSLQLLSSSSLPGLGLEQD
RQGPEESDEFQS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU51121
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Remove TRAF6 gene from the plasmid DU46823 and introduce IRAK1 [K134R][K180R] human gene