cDNA Clone IKK alpha

• Plasmid: pFastbac Dual IKK1 6HIS Abin2
• Parent Plasmid: pFBDM
• Expressed: 6HIS Abin2 + IKKa
• Genbank: O15111
• Species: Human

Sequence of Insert

ggctcgagGAAACCATGGAGCGGCCCCCGGGGCTGCGGCCGGGCGCGGGC
GGGCCCTGGGAGATGCGGGAGCGGCTGGGCACCGGCGGCTTCGGGAACGT
CTGTCTGTACCAGCATCGGGAACTTGATCTCAAAATAGCAATTAAGTCTT
GTCGCCTAGAGCTAAGTACCAAAAACAGAGAACGATGGTGCCATGAAATC
CAGATTATGAAGAAGTTGAACCATGCCAATGTTGTAAAGGCCTGTGATGT
TCCTGAAGAATTGAATATTTTGATTCATGATGTGCCTCTTCTAGCAATGG
AATACTGTTCTGGAGGAGATCTCCGAAAGCTGCTCAACAAACCAGAAAAT
TGTTGTGGACTTAAAGAAAGCCAGATACTTTCTTTACTAAGTGATATAGG
GTCTGGGATTCGATATTTGCATGAAAACAAAATTATACATCGAGATCTAA
AACCTGAAAACATAGTTCTTCAGGATGTTGGTGGAAAGATAATACATAAA
ATAATTGATCTGGGATATGCCAAAGATGTTGATCAAGGAAGTCTGTGTAC
ATCTTTTGTGGGAACACTGCAGTATCTGGCCCCAGAGCTCTTTGAGAATA
AGCCTTACACAGCCACTGTTGATTATTGGAGCTTTGGGACCATGGTATTT
GAATGTATTGCTGGATATAGGCCTTTTTTGCATCATCTGCAGCCATTTAC
CTGGCATGAGAAGATTAAGAAGAAGGATCCAAAGTGTATATTTGCATGTG
AAGAGATGTCAGGAGAAGTTCGGTTTAGTAGCCATTTACCTCAACCAAAT
AGCCTTTGTAGTTTAATAGTAGAACCCATGGAAAACTGGCTACAGTTGAT
GTTGAATTGCGACCCTCAGCAGAGAGGAGGACCTGTTGACCTTACTTTGA
AGCAGCCAAGATGTTTTGTATTAATGGATCACATTTTGAATTTGAAGATA
GTACACATCCTAAATATGACTTCTGCAAAGATAATTTCTTTTCTGTTACC
ACCTGATGAAAGTCTTCATTCACTACAGTCTCGTATTGAGCGTGAAACTG
GAATAAATACTGGTTCTCAAGAACTTCTTTCAGAGACAGGAATTTCTCTG
GATCCTCGGAAACCAGCCTCTCAATGTGTTCTAGATGGAGTTAGAGGCTG
TGATAGCTATATGGTTTATTTGTTTGATAAAAGTAAAACTGTATATGAAG
GGCCATTTGCTTCCAGAAGTTTATCTGATTGTGTAAATTATATTGTACAG
GACAGCAAAATACAGCTTCCAATTATACAGCTGCGTAAAGTGTGGGCTGA
AGCAGTGCACTATGTGTCTGGACTAAAAGAAGACTATAGCAGGCTCTTTC
AGGGACAAAGGGCAGCAATGTTAAGTCTTCTTAGATATAATGCTAACTTA
ACAAAAATGAAGAACACTTTGATCTCAGCATCACAACAACTGAAAGCTAA
ATTGGAGTTTTTTCACAAAAGCATTCAGCTTGACTTGGAGAGATACAGCG
AGCAGATGACGTATGGGATATCTTCAGAAAAAATGCTAAAAGCATGGAAA
GAAATGGAAGAAAAGGCCATCCACTATGCTGAGGTTGGTGTCATTGGATA
CCTGGAGGATCAGATTATGTCTTTGCATGCTGAAATCATGGAGCTACAGA
AGAGCCCCTATGGAAGACGTCAGGGAGACTTGATGGAATCTCTGGAACAG
CGTGCCATTGATCTATATAAGCAGTTAAAACACAGACCTTCAGATCACTC
CTACAGTGACAGCACAGAGATGGTGAAAATCATTGTGCACACTGTGCAGA
GTCAGGACCGTGTGCTCAAGGAGCTGTTTGGTCATTTGAGCAAGTTGTTG
GGCTGTAAGCAGAAGATTATTGATCTACTCCCTAAGGTGGAAGTGGCCCT
CAGTAATATCAAAGAAGCTGACAATACTGTCATGTTCATGCAGGGGAAAA
GGCAGAAAGAAATATGGCATCTCCTTAAAATTGCATGTACACAGAGTTCT
GCCCGGTCCCTTGTAGGATCCAGTCTAGAAGGTGCAGTAACCCCTCAGAC
ATCAGCATGGCTGCCCCCGACTTCAGCAGAACATGATCATTCTCTGTCAT
GTGTGGTAACTCCTCAAGATGGGGAGACTTCAGCACAAATGATAGAAGAA
AATTTGAACTGCCTTGGCCATTTAAGCACTATTATTCATGAGGCAAATGA
GGAACAGGGCAATAGTATGATGAATCTTGATTGGAGTTGGTTAACAGAAT
GAggtacccc

Amino Acid Sequence

MERPPGLRPGAGGPWEMRERLGTGGFGNVCLYQHRELDLKIAIKSCRLEL
STKNRERWCHEIQIMKKLNHANVVKACDVPEELNILIHDVPLLAMEYCSG
GDLRKLLNKPENCCGLKESQILSLLSDIGSGIRYLHENKIIHRDLKPENI
VLQDVGGKIIHKIIDLGYAKDVDQGSLCTSFVGTLQYLAPELFENKPYTA
TVDYWSFGTMVFECIAGYRPFLHHLQPFTWHEKIKKKDPKCIFACEEMSG
EVRFSSHLPQPNSLCSLIVEPMENWLQLMLNCDPQQRGGPVDLTLKQPRC
FVLMDHILNLKIVHILNMTSAKIISFLLPPDESLHSLQSRIERETGINTG
SQELLSETGISLDPRKPASQCVLDGVRGCDSYMVYLFDKSKTVYEGPFAS
RSLSDCVNYIVQDSKIQLPIIQLRKVWAEAVHYVSGLKEDYSRLFQGQRA
AMLSLLRYNANLTKMKNTLISASQQLKAKLEFFHKSIQLDLERYSEQMTY
GISSEKMLKAWKEMEEKAIHYAEVGVIGYLEDQIMSLHAEIMELQKSPYG
RRQGDLMESLEQRAIDLYKQLKHRPSDHSYSDSTEMVKIIVHTVQSQDRV
LKELFGHLSKLLGCKQKIIDLLPKVEVALSNIKEADNTVMFMQGKRQKEI
WHLLKIACTQSSARSLVGSSLEGAVTPQTSAWLPPTSAEHDHSLSCVVTP
QDGETSAQMIEENLNCLGHLSTIIHEANEEQGNSMMNLDWSWLTE*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU51358
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Cloned using3' Xho1 from pSC-b